E. Otros países
2.4. La descentralización como motor del desarrollo económico
2.4.2. La descentralización y el desarrollo local
Based on their conservation, many plant miRNA-target relationships are under strong evolutionary pressure, where the relationship has been finely modulated to play specific and essential roles in plant development. However, our knowledge of how these miRNAs are incorporated into plant regulatory networks is still very limited. Illustrated by this thesis, through the molecular and functional analyses of miR159-MYB module in the Arabidopsis rosette, the miR159-mediated silencing of MYB33/65 expression was found constitutive, ubiquitous and functionally active during rosette growth. In addition, miR159-mediated silencing of MYB33/65 in the rosette appeared extremely robust; under no examined conditions could this silencing be perturbed. Finally, the pathway appeared extensively networked in rosettes based on the large number of mir159ab
revertants recovered in a suppression screen. These intriguing findings have increased our understanding of this module in rosettes, but raises further questions about its functional role. Finding the answer could be very difficult, as all attempts to disrupt the miR159 function in rosettes did not result in an obvious morphological outcome. Hopefully, in near future, elucidating the mutations identified in the EMS-based
mir159ab revertant screen will provide more information of the miR159-MYB role in rosettes. Likewise, by using the methods (e.g. CP1 marker) and mutants (e.g.
191 be greatly facilitated. Last but not least, via exploiting miRNA SPs for inhibiting miRNA functions in Arabidopsis, many broader questions in relation to how the miRNA-target interactions are modulated in plants have also been raised. Identifying these modulating factors by modifying the miRNA decoys into versions resembling different structural/sequence features of miRNA endogenous targets, may be an approach to address these questions and hence should be considered for further investigation.
192
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