Fly stocks
Table 7 Fly stocks
genotype Description/use source
w1118 w- flies Bloomington Stock Center
yw X; Pin/CyOy+ II balancer 2 nd Barry Dickson yw/Yhshid X; Pin/CyOy+ II balancer 2 nd
virgin collection Barry Dickson yw X;
MKRS/TM6By+ III balancer 3rd Barry Dickson yw/Yhshid X;
MKRS/TM6By+ III balancer 3 rd
virgin collection Barry Dickson yw X;
Elp/CyO Kr-G U-GFP II; Ki/TM3 Kr-G U-GFP III
double balancer Gaia Tavosanis w/Yy+ X;
nub b Sco It stw/CyO II;
MKRS/TM6B III double balancer Bloomington Stock Center yw eyFLP2 C-lacZ X;
M(3)i[55] FRT80B/TM6By+ III 3
rd left, cell lethal Barry Dickson
yw eyFLP2 Rh1-τlacZ X;
M(3)i[55] FRT80B/TM3Sery+ III Rh1: R1-R6 marker Barry Dickson w eyFLP2 Rh6-mCD8GFPmyc X;
M(3)i[55] FRT80/TM3 III Rh6: adult R8 marker, stains 70% Barry Dickson w eyFLP2 Rh4-mCD8GFPmyc X;
M(3)i[55] FRT80/TM3 III Rh4: R7 marker stains 70% Barry Dickson yw eyFLP2 C-lacZ X;
gogo[D869]/TM6By+ III gogo mutant allele Takashi Suzuki yw eyFLP2 C-lacZ X;
gogo[D1600]/TM6By+ III gogo mutant allele Takashi Suzuki
yw eyFLP2 C-lacZ X;
Materials and Methods yw eyFLP2 C-lacZ X; FRT80 tub-GAL80 GMR-mKO M(3)i[55]/TM6By+ III GMR-mKO: R1-R8 marker,
Gogo AB staining Satoko Suzuki elav-Gal4 hsFLP UAS-mCD8GFP X;
GMR-KO tub-Gal80 FRT80/TM6By+ III cMARCM Satoko Suzuki
yw X;
ey1xFLP/CyO II;
gogo[D869] FRT80 III
cMARCM Satoko Suzuki yw X;
ey1xFLP/CyO II;
gogo[D869] FRT80 UAS-gogoFL-3B/
TM6By+ III
cMARCM Satoko Suzuki yw X;
ey1xFLP/CyO II;
gogo[D869] FRT80 UAS-gogoΔC-3B/
TM6By+ III
cMARCM Satoko Suzuki
yw X;
1xeyFLP/CyO;
GMR-KO tub-Gal80 FRT80/TM6By+ III
R8 bundling
phenotype in larvae, small mutant clones in pupae
Satoko Suzuki yw GMR-FLP X;
Gla/CyO II MARCM clones in R7 Larry Zipursky w eyFLP2 Rh6-mCD8GFPmyc X ;
GMR-KO Gal80 FRT80/TM3 III
making small clones, label R8 and WT cells
in adults Takashi Suzuki w eyFLP2 Rh6-mCD8GFPmyc X;
M(3)i[55] cl GMR-KO Gal80 FRT80/TM3 III
making large clones, label R8 and WT cells
inadults Takashi Suzuki
yw PM181-Gal4 X R7 specific Gal4 Bloomington Stock Center Yw X;
Pin/Cyo II;
UAS-mCD8GFP III
expression of GFP
localized to axons Bloomington Stock Center UAS-GFP:lacZnls II gogoexpression analysis -Gal4 Bloomington Stock Center UAS-GFP:lacZnls III gogoexpression analysis -Gal4 Bloomington Stock Center GMR-gogo-FLmyc II rescue experiments Stephan Ohler
GMR-gogo-ΔCmyc II rescue experiments Stephan Ohler UAS-gogo-FLmyc-T1 II Gogo OE Barry Dickson UAS-gogo-FLmyc-T2 II
R8 rescue, gogo2-
Gal4 rescue, Gogo
OE, Western Stephan Ohler UAS-gogo-FLmyc-2B II Gogo OE, Western Stephan Ohler UAS-gogo-FLmyc-3A III Gogo OE, Western Stephan Ohler UAS-gogo-FLmyc-3B III cMARCM, R7 rescue, Gogo OE, Western Stephan Ohler UAS-gogo-FLmyc-3C III Gogo OE, Western Stephan Ohler
Constructed Fly stocks
Table 8 Constructed Fly stocks
genotype Description/use
yw/yw eyFLP2 C-lacZ X; ato-τ-myc II;
gogo[D869] FRT80/TM6By+ III
ato: R8 marker, mutant R8 phenotype analysis in larvae
yw eyFLP2 C-lacZ X; GMR-gogo-FL II;
gogo[D869] FRT80/TM6By+ III
transheterozygous rescue yw eyFLP2 C-lacZ X;
GMR-gogo-ΔC II;
gogo[D869] FRT80/TM6By+ III
transheterozygous rescue w X;
ato-τ-myc gogo[D1600] FRT80/TM6By+ III transheterozygous rescue
ey3.5FLP II;
M(3)i[55] FRT80/TM6By+ III
clean ey3.5FLP from Iris Salecker, no brain clones, EM
yw eyFLP2 C-lacZ X; 109-68Gal4/Cyo;
M(3)i[55] FRT80/TM6By+ III R8 rescue yw/yw eyFLP2 C-lacZ X;
Caps-Gal4 gogo[D869] FRT80/TM6By+ III R8 rescue
yw/yw eyFLP2 C-lacZ X; UAS-Gogo-FLT2/Cyo II;
Caps-Gal4 gogo[D869] FRT80/TM6By+ III
R8 rescue yw X;
GMR-Gal4/CyO II;
gogo[D869] FRT80/TM6By+ III
positive control for gogo enhancer
fragment rescue yw X;
UAS-gogo-FL-T2/Cyo II;
gogo[D1600] FRT80/TM6By+ III
gogo enhancer fragment rescue
yw X;
gogo2-Gal4-T1 II;
gogo[D869] FRT80/TM6By+ III
gogo enhancer fragment rescue
yw eyFLP2 C-lacZ X; Gal80 FRT80/TM6By+ III
Replacement of non-functional stock in Bloomington stock collection
w X;
PanR7-Gal4 UAS-SybGFP/CyO II;
Gal80 FRT80/TM6B III R7 mutants yw X;
UAS-Gogo−ΔC-3B gogo[D869] FRT80/TM6By+ cMARCM
yw X;
UAS-Gogo−FL-3B gogo[D869] FRT80/TM6By+ R7 rescue, cMARCM
yw eyFLP2 C-lacZ X; ato-t-myc gogo[D869]
FRT80/TM6By+ pupal stages R8, R8 bundling in larvae yw eyFLP2 C-lacZ X, ato-t-myc FRT80/TM6By+ pupal stages R8, R8 bundling in larvae
Materials and Methods
Generated Transgenic fly lines
Table 9 Generated transgenic flies
Transgenic line Injected Plasmid Description
GMR-gogoΔN::myc-T1 II GMR-gogoΔN::myc-T2 II GMR-gogoΔN::myc-T3 II pGMR-gogoΔN::myc Gogo expression in R1-R8 [SP]1-54 [Cterm]689-1272 GMR-gogoΔN-A::myc-T1 II GMR-gogoΔN-A::myc-T2 II GMR-gogoΔN-A::myc-T3 II pGMR-gogoΔN-A::myc Gogo expression in R1-R8 [SP]1-54 [CUB+Cterm]535-1272 GMR-gogoΔN-B::myc-T1 II GMR-gogoΔN-B::myc-T2 II GMR-gogoΔN-B::myc-T3 II pGMR-gogoΔN-B::myc Gogo expression in R1-R8 [SP+GOGO]1-457 [Cterm]689-1272 GMR-gogoΔN-C::myc-T1 II GMR-gogoΔN-C::myc-T2 II GMR-gogoΔN-C::myc-T3 II pGMR-gogoΔN-C::myc Gogo expression in R1-R8 [SP]1-54 [Tsp1]458-534 [Cterm]689-1272 GMR-gogoΔN-D::myc-T1 II GMR-gogoΔN-D::myc-T2 II GMR-gogoΔN-D::myc-T3 II pGMR-gogoΔN-D::myc Gogo expression in R1-R8 [SP]1-54 [Tsp1+CUB+Cterm]458-1272 GMR-gogoΔN-E::myc-T1 II GMR-gogoΔN-E::myc-T2 II GMR-gogoΔN-E::myc-T3 II pGMR-gogoΔN-E::myc Gogo expression in R1-R8 [SP+GOGO] 1-457 [CUB+Cterm]535-1272 GMR-gogoΔN-F::myc-T1 II GMR-gogoΔN-F::myc-T2 II GMR-gogoΔN-F::myc-T3 II pGMR-gogoΔN-F::myc Gogo expression in R1-R8 [SP+GOGO+Tsp1]1-534 [Cterm]689-1272 GMR-gogoΔN-G::myc-T1 II GMR-gogoΔN-G::myc-T3 II GMR-gogoΔN-G::myc-T4 II pGMR-gogoΔN-G::myc Gogo expression in R1-R8 [SP]1-54 [GOGO+Tsp1]204-534 [Cterm]689-1272 GMR-gogoΔN-H::myc-T3 II GMR-gogoΔN-H::myc-T4 II GMR-gogoΔN-H::myc-T5 II pGMR-gogoΔN-H::myc Gogo expression in R1-R8 [SP] 1-54 [partial-GOGO+Tsp1]278-534 [Cterm]689-1272. gogo1-Gal4-T2 gogo1-Gal4-T3 gogo1-Gal4-T5 pgogo1-Gal4
Potential gogo enhancer
fragment fused to Gal4
gogo2-Gal4-T1 II (closer examined in experiments)
gogo2-Gal4-T4 II gogo2-Gal4-T11 II
pgogo2-Gal4
Potential gogo enhancer
fragment fused to Gal4
gogo locus:1st intron gogo3-Gal4-T2
gogo3-Gal4-T3 gogo3-Gal4-T4
pgogo3-Gal4
Potential gogo enhancer
fragment fused to Gal4
gogo locus:1st intron gogo4-Gal4-T2
gogo4-Gal4-T3 gogo4-Gal4-T4
pgogo4-Gal4
Potential gogo enhancer fragment fused to Gal4
gogo locus:1st intron