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Fly stocks

Table 7 Fly stocks

genotype Description/use source

w1118 w- flies Bloomington Stock Center

yw X; Pin/CyOy+ II balancer 2 nd Barry Dickson yw/Yhshid X; Pin/CyOy+ II balancer 2 nd

virgin collection Barry Dickson yw X;

MKRS/TM6By+ III balancer 3rd Barry Dickson yw/Yhshid X;

MKRS/TM6By+ III balancer 3 rd

virgin collection Barry Dickson yw X;

Elp/CyO Kr-G U-GFP II; Ki/TM3 Kr-G U-GFP III

double balancer Gaia Tavosanis w/Yy+ X;

nub b Sco It stw/CyO II;

MKRS/TM6B III double balancer Bloomington Stock Center yw eyFLP2 C-lacZ X;

M(3)i[55] FRT80B/TM6By+ III 3

rd left, cell lethal Barry Dickson

yw eyFLP2 Rh1-τlacZ X;

M(3)i[55] FRT80B/TM3Sery+ III Rh1: R1-R6 marker Barry Dickson w eyFLP2 Rh6-mCD8GFPmyc X;

M(3)i[55] FRT80/TM3 III Rh6: adult R8 marker, stains 70% Barry Dickson w eyFLP2 Rh4-mCD8GFPmyc X;

M(3)i[55] FRT80/TM3 III Rh4: R7 marker stains 70% Barry Dickson yw eyFLP2 C-lacZ X;

gogo[D869]/TM6By+ III gogo mutant allele Takashi Suzuki yw eyFLP2 C-lacZ X;

gogo[D1600]/TM6By+ III gogo mutant allele Takashi Suzuki

yw eyFLP2 C-lacZ X;

Materials and Methods yw eyFLP2 C-lacZ X; FRT80 tub-GAL80 GMR-mKO M(3)i[55]/TM6By+ III GMR-mKO: R1-R8 marker,

Gogo AB staining Satoko Suzuki elav-Gal4 hsFLP UAS-mCD8GFP X;

GMR-KO tub-Gal80 FRT80/TM6By+ III cMARCM Satoko Suzuki

yw X;

ey1xFLP/CyO II;

gogo[D869] FRT80 III

cMARCM Satoko Suzuki yw X;

ey1xFLP/CyO II;

gogo[D869] FRT80 UAS-gogoFL-3B/

TM6By+ III

cMARCM Satoko Suzuki yw X;

ey1xFLP/CyO II;

gogo[D869] FRT80 UAS-gogoΔC-3B/

TM6By+ III

cMARCM Satoko Suzuki

yw X;

1xeyFLP/CyO;

GMR-KO tub-Gal80 FRT80/TM6By+ III

R8 bundling

phenotype in larvae, small mutant clones in pupae

Satoko Suzuki yw GMR-FLP X;

Gla/CyO II MARCM clones in R7 Larry Zipursky w eyFLP2 Rh6-mCD8GFPmyc X ;

GMR-KO Gal80 FRT80/TM3 III

making small clones, label R8 and WT cells

in adults Takashi Suzuki w eyFLP2 Rh6-mCD8GFPmyc X;

M(3)i[55] cl GMR-KO Gal80 FRT80/TM3 III

making large clones, label R8 and WT cells

inadults Takashi Suzuki

yw PM181-Gal4 X R7 specific Gal4 Bloomington Stock Center Yw X;

Pin/Cyo II;

UAS-mCD8GFP III

expression of GFP

localized to axons Bloomington Stock Center UAS-GFP:lacZnls II gogoexpression analysis -Gal4 Bloomington Stock Center UAS-GFP:lacZnls III gogoexpression analysis -Gal4 Bloomington Stock Center GMR-gogo-FLmyc II rescue experiments Stephan Ohler

GMR-gogo-ΔCmyc II rescue experiments Stephan Ohler UAS-gogo-FLmyc-T1 II Gogo OE Barry Dickson UAS-gogo-FLmyc-T2 II

R8 rescue, gogo2-

Gal4 rescue, Gogo

OE, Western Stephan Ohler UAS-gogo-FLmyc-2B II Gogo OE, Western Stephan Ohler UAS-gogo-FLmyc-3A III Gogo OE, Western Stephan Ohler UAS-gogo-FLmyc-3B III cMARCM, R7 rescue, Gogo OE, Western Stephan Ohler UAS-gogo-FLmyc-3C III Gogo OE, Western Stephan Ohler

Constructed Fly stocks

Table 8 Constructed Fly stocks

genotype Description/use

yw/yw eyFLP2 C-lacZ X; ato-τ-myc II;

gogo[D869] FRT80/TM6By+ III

ato: R8 marker, mutant R8 phenotype analysis in larvae

yw eyFLP2 C-lacZ X; GMR-gogo-FL II;

gogo[D869] FRT80/TM6By+ III

transheterozygous rescue yw eyFLP2 C-lacZ X;

GMR-gogo-ΔC II;

gogo[D869] FRT80/TM6By+ III

transheterozygous rescue w X;

ato-τ-myc gogo[D1600] FRT80/TM6By+ III transheterozygous rescue

ey3.5FLP II;

M(3)i[55] FRT80/TM6By+ III

clean ey3.5FLP from Iris Salecker, no brain clones, EM

yw eyFLP2 C-lacZ X; 109-68Gal4/Cyo;

M(3)i[55] FRT80/TM6By+ III R8 rescue yw/yw eyFLP2 C-lacZ X;

Caps-Gal4 gogo[D869] FRT80/TM6By+ III R8 rescue

yw/yw eyFLP2 C-lacZ X; UAS-Gogo-FLT2/Cyo II;

Caps-Gal4 gogo[D869] FRT80/TM6By+ III

R8 rescue yw X;

GMR-Gal4/CyO II;

gogo[D869] FRT80/TM6By+ III

positive control for gogo enhancer

fragment rescue yw X;

UAS-gogo-FL-T2/Cyo II;

gogo[D1600] FRT80/TM6By+ III

gogo enhancer fragment rescue

yw X;

gogo2-Gal4-T1 II;

gogo[D869] FRT80/TM6By+ III

gogo enhancer fragment rescue

yw eyFLP2 C-lacZ X; Gal80 FRT80/TM6By+ III

Replacement of non-functional stock in Bloomington stock collection

w X;

PanR7-Gal4 UAS-SybGFP/CyO II;

Gal80 FRT80/TM6B III R7 mutants yw X;

UAS-Gogo−ΔC-3B gogo[D869] FRT80/TM6By+ cMARCM

yw X;

UAS-Gogo−FL-3B gogo[D869] FRT80/TM6By+ R7 rescue, cMARCM

yw eyFLP2 C-lacZ X; ato-t-myc gogo[D869]

FRT80/TM6By+ pupal stages R8, R8 bundling in larvae yw eyFLP2 C-lacZ X, ato-t-myc FRT80/TM6By+ pupal stages R8, R8 bundling in larvae

Materials and Methods

Generated Transgenic fly lines

Table 9 Generated transgenic flies

Transgenic line Injected Plasmid Description

GMR-gogoΔN::myc-T1 II GMR-gogoΔN::myc-T2 II GMR-gogoΔN::myc-T3 II pGMR-gogoΔN::myc Gogo expression in R1-R8 [SP]1-54 [Cterm]689-1272 GMR-gogoΔN-A::myc-T1 II GMR-gogoΔN-A::myc-T2 II GMR-gogoΔN-A::myc-T3 II pGMR-gogoΔN-A::myc Gogo expression in R1-R8 [SP]1-54 [CUB+Cterm]535-1272 GMR-gogoΔN-B::myc-T1 II GMR-gogoΔN-B::myc-T2 II GMR-gogoΔN-B::myc-T3 II pGMR-gogoΔN-B::myc Gogo expression in R1-R8 [SP+GOGO]1-457 [Cterm]689-1272 GMR-gogoΔN-C::myc-T1 II GMR-gogoΔN-C::myc-T2 II GMR-gogoΔN-C::myc-T3 II pGMR-gogoΔN-C::myc Gogo expression in R1-R8 [SP]1-54 [Tsp1]458-534 [Cterm]689-1272 GMR-gogoΔN-D::myc-T1 II GMR-gogoΔN-D::myc-T2 II GMR-gogoΔN-D::myc-T3 II pGMR-gogoΔN-D::myc Gogo expression in R1-R8 [SP]1-54 [Tsp1+CUB+Cterm]458-1272 GMR-gogoΔN-E::myc-T1 II GMR-gogoΔN-E::myc-T2 II GMR-gogoΔN-E::myc-T3 II pGMR-gogoΔN-E::myc Gogo expression in R1-R8 [SP+GOGO] 1-457 [CUB+Cterm]535-1272 GMR-gogoΔN-F::myc-T1 II GMR-gogoΔN-F::myc-T2 II GMR-gogoΔN-F::myc-T3 II pGMR-gogoΔN-F::myc Gogo expression in R1-R8 [SP+GOGO+Tsp1]1-534 [Cterm]689-1272 GMR-gogoΔN-G::myc-T1 II GMR-gogoΔN-G::myc-T3 II GMR-gogoΔN-G::myc-T4 II pGMR-gogoΔN-G::myc Gogo expression in R1-R8 [SP]1-54 [GOGO+Tsp1]204-534 [Cterm]689-1272 GMR-gogoΔN-H::myc-T3 II GMR-gogoΔN-H::myc-T4 II GMR-gogoΔN-H::myc-T5 II pGMR-gogoΔN-H::myc Gogo expression in R1-R8 [SP] 1-54 [partial-GOGO+Tsp1]278-534 [Cterm]689-1272. gogo1-Gal4-T2 gogo1-Gal4-T3 gogo1-Gal4-T5 pgogo1-Gal4

Potential gogo enhancer

fragment fused to Gal4

gogo2-Gal4-T1 II (closer examined in experiments)

gogo2-Gal4-T4 II gogo2-Gal4-T11 II

pgogo2-Gal4

Potential gogo enhancer

fragment fused to Gal4

gogo locus:1st intron gogo3-Gal4-T2

gogo3-Gal4-T3 gogo3-Gal4-T4

pgogo3-Gal4

Potential gogo enhancer

fragment fused to Gal4

gogo locus:1st intron gogo4-Gal4-T2

gogo4-Gal4-T3 gogo4-Gal4-T4

pgogo4-Gal4

Potential gogo enhancer fragment fused to Gal4

gogo locus:1st intron