our model for transcellular Ca2+ transport allows the estimation of the number of TRPV5
channels in the membrane. A more reliable estimation can be obtained when [Ca2+] in the TRPV5 pore has been determined. Future experiments that include fusion of fluorescent Ca2+
indicator proteins to the channel complex in combination with total internal reflection microscopy should provide this knowledge. In addition, this approach will provide alternatives to determine Ca2+ fluxes through single TRPV5 channels as recently described for
other Ca2+ channels (47). Finally, besides TRPV5 and TRPV6 function in the tissues that
contribute to the Ca2+ balance imminent research will spread out to other tissues, including
inner ear, where these channels contribute to physiological processes other than the maintenance of the Ca2+ balance. In summary, the findings described in this thesis open new
perspectives about the physiological function of TRPV5 and TRPV6 and pinpoint to novel associated protein partners controlling apical Ca2+ influx in Ca2+-transporting epithelia.
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