NIVEL MORFOSINTACTICO

Rationale: The p38 MAPK pathways regulate CDH1 and TJP1 in response to stress via SNAI1 (Grotegut et al., 2006; Lan et al., 2006). Furthermore, SNAI1 and SNAI2 are involved in regulating the Na/K-ATPase, another blastocyst formation associated protein (Espineda et al., 2004). However, little is known about the regulation of Snai1I and Snai2 during preimplantation development.

Hypothesis: The p38 MAPK pathway regulates blastocyst development by signaling through known targets, SNAI1 and potentially SNAI2, which in turn regulate CDH1, TJP1, OCCLUDIN and ATP1B1.

Objective: Characterize SNAI1 and SNAI2 expression and localization throughout preimplantation development.

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