In the present study screening of biological activities using combined plant extracts was perfomed on the microorganism under study. Surprisingly all methanol and acetone extracts of combined plant mixtures displayed zones of colouration of the plant extracts with no antibacterial activities on S. typhimurium and E. coli.
All extracts of a mixture of A. digitata: R. communis inhibited S. aureus. A combination of A. digitata: R. communis demonstrated antibacterial activities on S. aureus with the strongest inhibition on methanol and acetone extracts. Aqueous and methanol extracts of a mixture of A. digitata: C. papaya demonstrated slight inhibition on S. aureus. No
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antibacterial activities were noted on acetone extract of A. digitata: C. papaya. Acetone extract of A. digitata: G. incanum combination exhibited strongest inhibition on S. aureus. All extracts of a mixture of A. digitata: B. pilosa displayed antibacterial activities with a strongest inhibition on acetone extract. All extracts of the mixtures of R. communis: G. incanum, B. pilosa: G. incanum, R. communis: M. indica, G. incanum: M. indica, B. pilosa: M. indica, C. papaya: M. indica, C. papaya: G. incanum demonstrated strongest inhibition on S. aureus. Extracts of a combination of R. communis: B. pilosa inhibited S. aureus. However aqueous extract of a combination of R. communis: B. pilosa showed slight inhibition on S. aureus. Extracts of a mixture of C. papaya: B. pilosa inhibited S. aureus with methanol extract demonstrating a slight inhibition. S. aureus was inhibited by extracts of a combination of C. papaya: R. communis with methanol and acetone extracts displaying a slight inhibition. Extracts of a mixture of all six medicinal plants inhibited S. aureus with methanol and acetone extracts displaying strongest inhibition.
In the present study, acetone extract of a combination of A. digitata: M. indica exhibited strong inhibition on P. aeruginosa with slight inhibition on aqueous and methanol extracts. Aqueous extract of a mixture of A. digitata: C. papaya showed inhibition on P. aeruginosa. However, no inhibition of methanol and acetone extracts of a combination of A. digitata: C. papaya was displayed on P. aeruginosa. All extracts of a mixture of A. digitata: G. incanum displayed antibacterial activities on P. aeruginosa. Nevertheless, acetone extract of a combination of A. digitata: G. incanum showed strongest inhibition on P. aeruginosa with water extract displaying slight inhibition. Aqueous extract of A. digitata: B. pilosa combination inhibited P. aeruginosa with no inhibition on methanol and acetone extracts. Aqueous extract of a mixture of R. communis: B. pilosa showed slight inhibition on P. aeruginosa. However, no inhibition of P. aeruginosa was displayed by methanol and acetone extracts of a combination of R. communis: B. pilosa. All extracts of a combination of R. communis: G. incanum displayed antibacterial activities on P. aeuginosa with methanol extract displaying slight inhibition. Antibacterial properties were noted on all extracts of a mixture of R. communis: M. indica with aqueous extract showing slight inhibition. All extracts of a combination of B. pilosa: M. indica showed inhibition of growth on P. aeruginosa. All extracts of a combination of B. pilosa: G. incanum demonstrated antibacterial activity on P. aeruginosa. Nevertheless, methanol extract of a
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combination of B. pilosa: G. incanum exhibited slight inhibition on P. aeruginosa. All extracts of a mixture of C. papaya: M. indica inhibited P. aeruginosa with aqueous extract demonstrating slight inhibition. Aqueous extract of a mixture of C. papaya: R. communis showed slight inhibition on P. aeruginosa. No inhibition of P. aeruginosa was exhibited by methanol and acetone extracts of a mixture of C. papaya: R. communis. No antibacterial properties were noted on P. aeruginosa on all extracts of a mixture of C. papaya: B. pilosa. P. aeruginosa was inhibited by all extracts of C. papaya: G. incanum combination but aqueous and methanol extracts demonstrated slight inhibition. All extracts of a combination of six medicinal plants under study demonstrated antibacterial properties on P. aeruginosa.
In the present study it was interesting to note that methanol and acetone extracts of all plant combinations under study exhibited no antibacterial activity on S. typhimurium with exception of some aqueous extracts. S. typhimurium was inhibited by aqueous extracts of mixtures of A. digitata: G. incanum, R. communis: G. incanum, B. pilosa: G. incanum, R. communis: M. indica, G. incanum: M. indica, B. pilosa: M. indica, C. papaya: M. indica, C. papaya: B. pilosa, C. papaya: R. communis, C. papaya: G. communis. Aqueous extracts displaying no antibacterial activities on S. typhimurium were A. digitata: R. communis, A. digitata: M. indica, A. digitata: C. papaya, A. digitata: B. pilosa, R. communis: B. pilosa. Aqueous extract of a mixture of six medicinal plants under study exhibited antibacterial activities on S. typhimurium.
Extracts of plant combination exhibiting no antibacterial activity on E. coli were A. digitata: M. indica, A. digitata: C. papaya, A. digitata: G. incanum, A. digitata: B. pilosa, R. communis: B. pilosa. Aqueous extracts of medicinal plant combinations of A. digitata: R. communis, R. communis: G. incanum, B. pilosa: G. incanum, R. communis: M. indica, G. incanum: M. indica, B. pilosa: M. indica, C. papaya: M. indica, C. papaya: B. pilosa, C. papaya: R. communis, C. papaya: G. incanum demonstrated antibacterial activities against E. coli. However, aqueous extract of a combination of A. digitata: R. communis displayed a slight inhibition on E. coli. Aqueous extract of a combination of all six medicinal plants under study showed antibacterial activity on E. coli with exception of methanol and acetone extracts.
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Extracts of plant combinations exhibiting no antifungal activities on C. albicans were A. digitata: R. communis, A. digitata: M. indica, A. digitata: C. papaya, C. papaya: R. communis, R. communis: M. indica and C. papaya: M. indica. Organic extracts of a combination of A. digitata: G. incanum displayed antifungal activity on C. albicans with methanol extract exhibiting slight inhibition. No inhibition of C. albicans was demonstrated by the aqueous extract of A. digitata: G. incanum. All extracts of a mixture of A. digitata: B. pilosa displayed antifungal activities on C. albicans with aqueous extract displaying the strongest activity. All extracts of a mixture of R. communis: G. incanum showed antifungal activity on C. albicans. However, a strongest activity of a combination of aqueous extract of R. communis: G. incanum was demonstrated on C. albicans. All extracts of a mixture of C. papaya: G. incanum displayed antifungal activity on C. albicans with a strongest activity on methanol extract. Methanol and acetone extracts of the combination of B. pilosa: M. indica inhibited C. albicans. Furthemore, C. albicans were inhibited by all extracts of a mixture of B. pilosa: G. incanum with a strongest inhibition on aqueous and methanol extracts. C. albicans demonstrated resistance against acetone and aqueous extracts of a combination of R. communis: B. pilosa. Methanol extract of a combination of R. communis: B. pilosa displayed antifungal activity on C. albicans. All extracts of a mixture of G. incanum: M. indica showed antifungal activity on C. albicans with acetone extract displaying a slight inhibition. Aqueous extract of C. papaya: B. pilosa did not exhibit antifungal activity on C. albicans, however, methanol and acetone extracts of C. papaya: B. pilosa displayed antifungal activity on C. albicans. All extracts of a mixture of six medicinal plants under study demonstrated antifungal activity on C. albicans.
In the present study aqueous and organic extracts of plant combinations demonstrating no antifungal activities on C. neoformans were A. digitata: R. communis, A. digitata: M. indica, A. digitata: C. papaya, C. papaya: R. communis. Aqueous and organic extracts of plant combinations exhibiting antifungal activities on C. neoformans were A. digitata: B. pilosa, R. communis: G. incanum, B. pilosa: G. incanum, G. incanum: M. indica, C. papaya: G. incanum. Organic extracts of R. communis: B. pilosa showed inhibition of growth on C. neoformans with acetone extract demonstrating slight inhibition. However, C. neoformans demonstrated resistance against aqueous extract of a mixture of R. communis: B. pilosa. Acetone extract of R. communis: M. indica exhibited slight inhibition
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on C. neoformans. No inhibition of growth was displayed on C. neoformans by aqueous and methanol extracts of a mixture of R. communis: M. indica. Acetone and methanol extracts of A. digitata: G. incanum showed antifungal activities on C. neoformans. Nevertheless, acetone extract of a mixture of A. digitata: G. incanum displayed slight inhibition on C. neoformans. Acetone extract of a mixture of C. papaya: M. indica demonstrated activity on C. neoformans. However, no antifungal activity of aqueous and methanol extracts of a mixture of C. papaya: M. indica was noted on C. neoformans. Methanol and acetone extracts of a mixture of C. papaya: B. pilosa inhibited C. neoformans. No antifungal activity was displayed on C. neoformans by aqueous extract of a mixture of C. papaya: B. pilosa. Acetone extract of a combination of all six medicinal plants inhibited the growth of C. neoformans. However, C. neoformans demonstrated resistance against aqueous and methanol extracts of a combination of all six medicinal plants.