MEMORIA CONSOLIDADA
19. Patrimonio neto atribuible a los socios de la matriz
We showed that the fibers yielding increased SCFA concentrations in the colonic lumen all increased the transcription of genes involved in metabolic processes associated with energy metabolism. Our correlation analysis demonstrated multiple relationships between the microbiota composition and these changing host gene expression patterns. In particular bacterial groups within Clostridium cluster XIVa positively correlated with genes involved in energy metabolism. Next to primary degrading bacterial species, this bacterial group is known to encompass many secondary fermenters, of which several have been shown to produce butyrate as their metabolic end product [57]. Unfortunately, and analogous to many other studies, the data presented here are based on single time-point measurements, and thereby fail to represent actual production or absorption rates of SCFA. Such flux data could give a considerable refinement to our understanding of the rate of production of butyrate by these bacteria and the actual levels of butyrate flux experienced by the colonic epithelia, respectively [58].
Conclusion
Our results provide a comprehensive overview on the effects of five fibers in the murine colon, which suggest that despite different source and composition, fermentable fibers are inducing a highly similar mucosal response that may at least be partially governed by Pparγ.
Acknowledgments
We would like to thank Dr Diederick Meyer (Sensus) and Dr Hans van der Saag (BioActor) for their kind gifts of inulin and oligofructose, respectively arabinoxylan.
This work was (co)financed by the Netherlands Consortium for Systems Biology (NCSB), which is part of the Netherlands Genomics Initiative / Netherlands Organization for Scientific Research.
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