PROCEDIMIENTO EXPERIMENTAL

P i * . 5 .1 .3 The r e la t io n s h i p between peak p r o d u c t i v it y

and th e t l a e ela p se d u n t i l the a c t i v i t y

a f t e r a c e r t a i n t i n e , when c e l l a c t i v i t y was l o s t and the e le c t ro n donor

was no lo n g e r lim it in g , the p r o d u c t iv it y o f the IB F -r e a c t o r and c e l l

a c t i v i t y approached the same v a lu e o f p o t e n t ia l a c t i v i t y .

The r e la t io n s h i p between peak p r o d u c t iv it y and h a l f l i f e in d ic a te s

th at peak p r o d u c t iv it y and/or the n et PO produced a f f e c t s the r a t e o f

in a c t iv a t io n o f c e l l s . By in c r e a s in g the PO p r o d u c t iv it y to more than

200 mU/mg c e l l s , in a c t i v ia t io n was a c c e le ra t e d and the h a l f l i f e was

shortened. 200 mU/mg c e l l s o f peak p r o d u c t iv it y way be a c r i t i c a l point

f o r the o th e r unknown in a c t i v a t i o n mechanism. I n the next S e ction ,

e f f e c t s o f e x t e r n a l PO con c e n tra tion on in a c t i v a t i o n o f c e l l s under the

co n d itio n s o f PO produ ction a r e stu d ie d .

5.5

under the C on ditions o f PO Production

The p r i n c i p a l reason f o r d e v e lop in g the IB F -r e a c t o r was to

in v e s t ig a t e the e f f e c t o f e x t r a c e ll u l a r PO c on ce n tration on the

in a c t i v a t i o n o f c e l l s under the con d ition s o f PO produ ction. From the

r e s u lt s o b ta in e d to d ate i t h as become c l e a r th a t the in a c t iv a t io n

appears to be due to the concerted e f f e c t between the e x t e rn a l PO and an

in t e r n a l u n id e n t i fie d e f f e c t fo llo w in g the o x id a tio n o f p ropylen e. To

in v e s t ig a t e the co n ce n tration o f the e x t r a c e ll u l a r PO c on ce n tration on

the in a c t i v a t i o n d u rin g PO p rodu ction from pro py len e, PO was added to

the r e a c t io n medium which a l s o contained pro py len e, oxygen and methanol.

5 -5 -1 Experim ental

To in v e s t ig a t e the e f f e c t o f e x t e rn a l PO c on ce n tration , the

re a c tio n medium co n ta in in g v a r ie d amounts o f PO and a ls o co n tain in g

I f PO which i s added e x t e r n a lly a c c e le ra t e d the in a c t i v a t i o n , then the

peak p r o d u c t i v it ie s a r e expected to decrease fo llo w in g the change over

o f aed iu a which does not co n tain PO.

5 .5 -2 R esu lts

The r e s u lt s a r e shown in F i g . 5 -5 -1 - No s i g n i f i c a n t e f f e c t o f PO on

a c c e le r a t io n o f in a c t iv a t io n was observed by th e tr e a t a e n t w ith 0 -

l.O aN PO f o r 5 a in u t e s , but when c e l l s were t r e a t e d w ith 1.9aM PO under

the con d ition s o f PO p rodu ction , in a c tiv a tio n was a c c e le ra t e d . The c e l l s

which were tr e a t e d w ith PO c o n ce n tration s ra n g in g fr o a 0 - l.OaM l o s t

about IOObU o v er the i n i t i a l 5 a in u t e s . T h is l o s s o f a c t i v i t y was

probably due to the o x id a tio n o f propylene. Although , the c e l l s which

were tr e a t e d w ith 1.9"N PO l o s t approxim ately 200aU o v er 5 a in u t e s . This

d iffe r e n c e o f IOObU o v er 5 a in u t e s appears to b e an e f f e c t o f the