Programa de trabajo

Coordinator: Prof. Y.D. Sharma

Major activities of the centre during the year 2012-13

BTIS provided support for three major activities of our department namely (i) Teaching: All practical classes of Bioinformatics and Computers module of M.Biotechnology (ii) Research: Active research was carried out in the field of infectious diseases including malaria & tuberculosis, cancer and cardiac diseases culminating in (iii) Publications, diagnostics and

patents.

Infrastructure & Scientific software packages:

We have one Sun server with Xenon processor and GcG software for research and teaching in Bioinformatics. Students, research scholars and faculty are using various scientific software like Labwork for Gel Analysis, GraphPad Prism for data analysis and End Note etc. Access to computers and accessories including round the clock internet facility for teaching, scientific research and publication to students, research scholars, office staff and faculty is being provided.

Teaching of Bioinformatics: A 4.5 credit point course module in bioinformatics is being run for M,Biotechnology students.

The students are imparted with hands on training in the use of various bioinformatics tools and packages. Experts are invited from various academic institutes/centres for delivering lectures on various topics in Bioinformatics to M.Biotech. students. Some of the collaborating institutes/centres include IGIB, New Delhi, SIT, JNU New Delhi, IIT Delhi, Deptt. of Biophysics, AIIMS, Computer Facility, AIIMS and Dept. Of NMR, AIIMS.

Software developed: The two-software packages Spectral Repeat Finder and My Pattern Finder developed by us are being

used by students for in silico analysis of research hypotheses and are finding support in experimental results.

Initiation of R&D activities in Bioinformatics:

In silico analysis of wet-lab. Data and hypothesis formulation using bioinformatics tools available on-line is an integral part

of all R&D activities being carried out in our department.

Manpower trained: BTIS is providing support to the M.Biotech. Teaching Programme, PhD scholars, MD/MS trainees,

WHO fellows, DBT Associates and summer trainees. The total strength of manpower trained is, therefore, greater than 447 till to-date.

Impact shown by centre on the growth of biotechnology and allied areas:

No. of M.Biotech students trained: 225

No. of PhD scholars passed out 42

No. of PhD scholars enrolled currently 16

No. of Post MD/MS professionals trained > 17

No. of summer trainees > 152

No. of WHO fellows trained 21

No. of papers published in 2012 18

No. of patents 16

Publications in 2012:

Papers published in peer-reviewed (Pub-Med indexed) journals in 2012:

1. R.K. Tyagi, Y.D. Sharma. Erythrocyte binding activity displayed by a selective group of Plasmodium vivax

tryptophan rich antigens is inhibited by patients’ antibodies. PLoS One, 7, e50754 (2012).

2. M. Zeeshan, H. Bora, Y.D. Sharma. Presence of memory T cells and naturally acquired antibodies in Plasmodium

vivax malaria-exposed individuals against a group of tryptophan-rich antigens with conserved sequences. J. Infect. Dis., 207, 175-85 (2012).

3. S. Garg, V. Saxena, V. Lumb, D. Pakalapati, P.A. Boopathi, A.K. Subudhi, S. Chowdhury, S.K. Kochar, D.K. Kochar, Y.D. Sharma, A. Das. Novel mutations in the antifolate drug resistance marker genes among Plasmodium vivax isolates exhibiting severe manifestations. Exp. Parasitol., 132, 410–6 (2012).

4. M. Zeeshan, M.T. Alam, S. Vinayak, H. Bora, R.K. Tyagi, M.S. Alam, V. Choudhary, P. Mittra, V. Lumb, P.K. Bharti, V. Udhayakumar, N. Singh, V. Jain, P.P. Singh, Y.D. Sharma. Genetic variation in the Plasmodium falciparum circumsporozoite protein in India and its relevance to RTS,S malaria vaccine. PloS One 7, e43430 (2012). 5. P.K. Bharti, M.M. Shukla, Y.D. Sharma and Neeru Singh. Genetic diversity in the block 2 region of the merozoite

surface protein-1 of Plasmodium falciparum in central India. Malaria J., 11, 78 (2012).

6. Y.D. Sharma. Molecular surveillance of drug resistant malaria in India. Curr. Sci., 102, 696-703 (2012).

7. V. Lumb, R. Madan, M.K. Das, V. Rawat, V. Dev, W. Khan, H. Khan and Y.D. Sharma. Differential genetic hitchhik- ing around mutant pfcrt alleles in Indian Plasmodium falciparum population. J. Antimicrob. Chemother., 67, 600-8 (2012).

8. S. Dhingra, K. Kaur, N.K. Taneja and J.S .Tyagi. DevR (DosR) binding peptide inhibits adaptation of Mycobac-

terium tuberculosis under hypoxia. FEMS Microbiol. Lett., 330, 66–71 (2012).

9. S. Haldar, N. Sankhyan, N. Sharma, A. Bansal, V. Jain, V.K. Gupta, M. Juneja, D. Mishra, A. Kapil, U.B. Singh, S. Gulati, V. Kalra, J.S. Tyagi. Detection of Mycobacterium tuberculosis GlcB or HspX Antigens or devR DNA Impacts the Rapid Diagnosis of Tuberculous Meningitis in Children. PLoS ONE, 7, e44630 (2012).

10. S. D. Majumdar, A. Vashist, S. Dhingra, R. Gupta, A. Singh, V. K. Challu, V. D. Ramanathan, P. Kumar and J. S. Tyagi. Appropriate DevR (DosR)-mediated signaling determines transcriptional response, hypoxic viability and virulence of Mycobacterium tuberculosis. PLoS One 7, e35847 (2012).

11. S. Das, P. Duggal, R. Roy, V. P. Myneedu, D. Behera, H. K. Prasad and A. Bhattacharya. Identification of Hot and Cold spots in genome of Mycobacterium tuberculosis using Shewhart Control Charts. Sci Rep., 2, 297 (2012). 12. M. Chaduvula, A. Murtaza, N. Misra, N. P. Narayan, V. Ramesh, H. K. Prasad, R. Rani, R. K. Chinnadurai and I.

Nath. Lsr2 peptides of Mycobacterium leprae show hierarchical responses in lymphoproliferative assays, with selective recognition by patients with anergic lepromatous leprosy. Infect. Immun., 80, 742-52 (2012).

13. V. K. Verma, V. Taneja, A. Jaiswal, S. Sharma, D. Behera, V. Sreenivas, S. S. Chauhan and H. K. Prasad. Prevalence, distribution and functional significance of the -237C to T polymorphism in the IL-12Rbeta2 promoter in Indian tuberculosis patients. PLoS One 7, e34355 (2012).

14. P. Gaur, G.A. Qadir, S. Upadhyay, A.K. Singh, N.K. Shukla and S.N. Das. Skewed immunological balance between Th17 (CD4+IL17A+) and Treg (CD4+CD25+ FOXP3+) cells in human oral squamous cell carcinoma. Cell. Oncol. 35, 335-43 (2012).

15. M.Y. Karimi , V. Kapoor, S.C. Sharma, S.N. Das. Genetic polymorphisms in FAS (CD95) and FAS ligand (CD178)

promoters and risk of tobacco-related oral carcinoma: gene-gene interactions in high-risk Indians., Cancer

Invest,1-6 (2012).

Contact details:

Name of the Coordinator: Prof. Y.D. Sharma

Postal Address: Dept. of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029.

Phone Number:26594609 Mobile: 9868397964 Fax: 26589286 Email:[email protected]