TALLER PINTURA
2. Marco Teórico
2.2 Bases teóricas
2.2.1 Contabilidad de Costos
2.2.1.6 Resolución Tributaria Fiscal N° 04289-8-
\ ..y; .7 .'7 : :'y .7/7 '7 7.-'T77Kÿ/:
gggagn : '8ee tab le 10 and 'il# and p la tes 21-4&^ l l 1. G lbberelllo aoid and lAA both.aooelerated tho rate o f the ' , growth roeponeo, G ib b orelllc aold in itia te d the most rapid . », reepoAse# the rc3aponee occurring in eoveral turlono' aftei* 4
o. '74:v#6[
daye# w hich ,is equivtilent to a c on tro l rooponae a t 20 0 , , . ' 3» Rlboee and mucroae hud l it tl o effect#
3'. Where turlono were exposed, to bhe bathin g oolutiono fo r a ^
longer perio d of time (mee tab le 11)# glb berelilc acid and lAA
ag ain ac celera ted th e growth reepbnbe# Sueroee a t the high est :
ooncen tration ^ao oelerated th e growth roeponec Y/hllnt rlb oeé had
ho c le ar effect# . . , '
pI8pU8pI0N% Thd in ter p re ta tion of the ruoulte lo lim ited by - * la<& of da ta concerning th e 'ra t es of peneti*ation of the above . ' 'compounds in to the tlBaues 'Of th e .turlona under khe experimental
oondltions# and also concerning the le v éla of endogenous , .
hormones# g'urthermoro th e ooncentratlono unod are very high#, ,1,. about 10~^$# However tho grov/th roaponee to such oolutionn . was normal end thus aq u a tic p la n ts may have high concen trations ^ of hormones p rese n t during th e rap id burs la of growth tlia t th ey /- A
exhibit# ' The turion e were by this time boginnlng growth in . ' .
the loch# and thus the stimulatory effeoto o f g lb b e re illo acid» ci . and .lAA, recorded above are only .acceleration oi\ th is growth* , « ..AJ
Qinoo gih b orellln a and poeeibly auxine are prooon^ n-.-’’ ■ ' ' % ■;,'!/>-;-V, 7 ' . “7 ,, - _ - ' . . :x7.. 7 7P; % 4 ' ' ■- ' ■ 't in the A 4 ^ turiona and nupply o f thoee hormonoe aocelerated tho I'qepom^o#-
then they may be Invblvod in the early otagoe o f growth of the
, , , , . ^
turione#' Other factors mày in it ia te the growth# but tho
" ^ ' ' 71
hormoneo may hovertheloee have a very important role# Tho ' 'j
Î ',:',|:^.7..-.;'.- . ' \ xA47.7 ,'7l7fr7 t-7 '. .7 77 ..=4; 4,;.7 7 - "' '7 ; .■ ■ X -7'. . v-’- ^ ' V7i.7\ ' ^ ^ ^
rap id ity o f the req>onee to g lb b e re illo acl^ may aimply r e fle ct %'e rapid rate o f penetration o f the tissu e# fo r with Oai:](fitrip49
staanalim McComb (1965) reported that (1A3 uptake into tho plant - 1
S # 8 ^ 7 ' '':7 '. /I
6 l,
waa h alf u aturato d In 15 minutes» \ lAA Is e as ily photolymed' by b lu e and red ligh t# and thue th ere may liave been some
breakdown' in solution* %he efft'-qt of th e lAA may have been
: 'orr'd^itmhlatibn of; *$6] % ' Ç '':'T /.' .'Yr p.vt A'l'V' ... . - ; "-" . ' Ï • 7 : :V
:i|7
!\ : ' ' - - ' - \ . A': ^ r / : ' -r
AIM: Since promotion of growth/b^ exogenous supply of IAA may W'' :d:lfeot tpn .méaiatéd^by, iM ^induced ;'etiiylene^ this experiment " ' .
stud ies ■ th # response: of the turions to à; rW ge -, of'lAA" solutions# ‘ EM:ERIALS:-- Tufions were Obtained from the Lake o f M entèith on
jranUafy -Sitk# 1974# ; !Po make up the lM (s61ût^ IAÀ:: was ' ■
fi r st d is s o lved in a. drop of ; absoluter'e#!^ ' -
METHOD; 'Five tarions were placed in to 25Pml* beakers con tainin g
•, ■ . -. ■ ■■■; •' **."5 0 ■ - '■ —B ■ :onO of.th e,,..i^llo w i% ;' 'd lstlllêd /waterÿ X iO / M.,.
IM -io"% '':aiiâ ^
To determ ine if supply of hormone* a t 5^0 could overcome ' . groy/th in hib ition . tarions '.were'h eld initial l y a t in th e dark* .. " Ho ' growth; response • occurred w ithin 1 h days ' and;/.as'\;this,. response -, ;'0 #
occurs w ithih 14 days in the loch à t a low; temperature it was con - v ; .:
s ide f ed th a t''th e , temperature imlght ; be ^ l:$mi tin g , : th e rpsponBU. to the Li ' su p p lied hormone# The turiohs were tra nsferred , to 20% in the
dark th ere fo re , and th e turions began gfowth within 3 days. ^ '.RESULTS s I;.-- The expe^plment \yas;;carried'.;out,’ from 20*2#% 3*3#74#
' Be suits mrè p resen ted in tab lé 12# . ' “
‘iHEATMEM , ' ' ^ ANGLEB ' , : HSAHS ± S.E, :
.OPNïROIjS ' V ' r " X,;:0$:30,iQ^W,0 , ~ ,l6 i7 p 9 -
lAii 1o"^Æ , ,, ; 61 *78,10k,96,55 76.8,,£ 5.52,
, IM 10""% - ' ; 2 5 ,
15»
15,27,51 . 26.6,.+, 5 ,8 9 . , 1 # 10-f% % - v,./:::,;;;;:8,W»3p,52,12- :24*$ $ 6.31y \:'ÏM 10:'^% ,, ' ; Ury 38,27»:#$24,0 2 6 * 4 # 7^81 ^ t* t est on means d iffere hces ind ica tes th a t only 1p % i'i/litre
lAA; had a s ignifica n t e f f ect on thé growth response#
TABX^ 12# -Qrowth response of.' tarions of bbtusifollU B '-MdiK' (Angles of ce n tral bud i'fom ho riz on tal) when held ,. in ba thin g so lu tions; of I t t a t 5^0 in the dark fo r \ ;i2;;':days# fôllçW éd by tra nsf er t d ^ in the dark# ÿ
63*
r *BlSCIISSlbli: ïn experiment 5 a t ,5^0■ when the tarions (12 [per 250ml# ) ' ' ' ' ' ' , ' . ^ \ ^ ' ' ' ' : /: '. /' were exposed to lAA "bathing eolutlona fo r '24hr, and then held a t .
5 0 and 8hr# illu min ation # the .growth reaponne ooourred a f ter 11-
'13. day8*. " In th is experiment 6' tarions were held in 250ml* lAA. \ % ' "ba' ' thing oolu' _ tion at 5 0 f" o o r 12 dayo# bu' ' . . t in the da' rk and nd reepbnae;,^ f
took place# l^enetratidn of the tinoue is u nlikely to have token
this time ,'hilt' tra ns p o rt of auxin and as sym étrie ro d le trlh u tlon may ^ -'i
however Wve been ra te -lim ited "by th e low temperature* Tranrf&r '
„ ; ' "o ' ' \ ' , T-
of the turions , s ti l l in' the dark# to 20 C# initia ted the res p onse ' *
' ' ' . , , ' ' ' '
within 3 days# b oth in th e c on tro ls and in the tarions exposed to
JAA "bathing so lu tions but oonkrols kept a t 5^0 in the dark bad h t i l l » "
noi: reeponded# This Ind ioatca th a t oven were ou ffio ie n t' a u xi n ^ t
p rese n t ondogenously# a t 5 0 the. response wou31i be very alow.
8inoe ,the groeo Vlaua3. growth response o ccurred 'w ithin 3 .days a t n . 20 0 the cellular responpo may have been Immediate. Furtherm ore
\ ~4 ^ , r
S ince '10 Müm was reqjuir^d to enlionoe.the growth response' a t
20^0, ethy len e may have boon' induced* "lAA stimula tion of ' r
' /I. ' . ' ' ' ' » ' '''
e thy le n e 'Byiithosis woo rep o rted by T ra tt & Goeeohl (1969) and y, ' "by Sargent e;(^, al (1974)# Sargohb and co-worl^ora 'have found th a t ' T
in SüjÜJfüB '^K .IO^^W, lAA cau&os an 11-fo ld increase^ in ethy len e
production# with maximum a f ter 9hr* This hypothesis could have
be e n ,tes Led "hym pplying motorod otliylene to th e tu rio n s o r by holding bhsm in SThkSL "G* T his was not .carried ou t a t thin sta g e
>in the thosio# fo r n eith er th e th eo re tical im p lica tions of ethy len e ^ ^ C In grotj^th# no r its use experimen tally were known in su f fic ie n t 6e tailjT
Changés ^n the so lub le carbohydrates in th e tarions in the earl^r phases of vis ib le growth are considered In bhc fo llo w in g . experimental onai^yseu* -
': ■ - - k : - !
çbÿrt'hÿ'/' ' 'à '' ' '
of. th e turlopm
(ai .In tro duction . .U arbo!W r% #o ... t-& ÿ' . .'TKiboee - ib-a.com ponent
'l^inoseAàâd %yl08.e.are' o.omi6a',oon8.titwntn*.Af.'fOellKW#ll /.y %;:^3ynme#rA^id(^(3; niooomo ond fruoto o o nra ;Wvo3ved in. ' - ' .' y.'-. - .-^r;;)'''.'-':.;^:-/.;/ /''/'A:;'''-, '
(%;
*n bbe frc o * *;xto lu ujnuts aro twrvnp nhu m^Uonw#
Thufi thoro 0)4' iidecuatu goac jul l'oanonr fo r ^ludyin^p
.. ■ '
'
a
o l i A Ïf
o o r b o m
K ti'acL
. Ic -v D -P l
a
i , l , m
L r. . 'i'hc .U -vc rtr. o i ’
üA I '
io- ' , ,Α|
A ïA .4 ::iV ^ î^ 'L ;.s A iA iâ A £ ;A lj4 /iÿt;i.,^ :-....i .. ,.':.:w ':jA 'v . :,_: ziîiis,
a
I
h y dr a b o n i n u cto a t lr p l . i ito w c r r o r u < c d c r e U to b c v , o r ü h i\b ^ ' ' ' vcsWgaLlng# toxicw rm ino: . ,, - J . <■.% .iUl A A '..:...4 1; - \ ." .. : -. A A : '" ': • ■.''/b#'A##ttAW^:the^e #Q^' partiti(^ ^ irofaructi^ti w,e u f / . A A - : , ' , '■
\ W r t # ! h v a r i Q ü 6 . \ p a i » t c o r tho plants: a t diffL-rnnb
I h i t i » 2.X.ÿ ..üo jv r i.J iîx -a 'liï^
fciîiso ». rtt, I'.v'i’ot, >iiu u .v fciin.:-:
i.-‘< iA 'i.'i-.'U î
i ’s?o.a
' - ' .
n f tb c fnlln w :,ic uuacIf.A c o ijca tcu UEi^ci^c.U -
règ àrd to in t er p r ét a tif . of the tihbole; and
k'Tr-:.V'AA ' À\A' ' ' - . A.
most 'Of the /e x trà éta are, th us from P* ,ob;bupi^9ll^^ the c,; t
l if e %!ôyole;,of wh$c%;i8 ..studied .in, de tail
tW indùotioii' andiFê.éorétion of' enzyme.%.A'Aÿ;
re ti re d fo r sugar formation# a nd 'fo r intorom m reion of su g ars,