Revisión de propuestas para la expansión de consultas basadas en modelos de

b

tfer

1od

allowo

t

aettle , the •fter

4eoaa••

, aa4 trea

waa r• eatt ·••••ral \laea \o

naoft all

p

tela aaterial a

e 11111

hlorlde t a\ o

l"eaala

tN• el:U'lieP

•• ·

!lle alUI'J of oell•loe•

•••

thea r

lata

a

••••••

ehroafloa

ool

( 20

u

4:1

...

r

•

.500

..

leaetla )

paoked

b7

,aeplJll

butter

o t 1

t

for

2 hours

ulq a

ic•

,.rte taltlo puap.

fto

pn an •

le ooataia1q

JOO

q

of ••rue

• el'lal,

fNall17

al••

• l * 1-aeroaptoe\h ol , waa

,..,ed

oato

•

top ol

the ool....

fk1a •••

followed b7

�Iter , .. ,. at \he rato

ot

'' ·�

vr froa

a

2

baaber sra4le t 4••1••

(�

a

l

�

tter

2

4n,.

2•ae"aptoe thaAOll

�

•1 'buffer, 2

4ro,-

2_..1'

aptoe,a.aaol

aDd

•

iu

o

101'1•• ••e

to 2

•

lafl ) wb.iob fed a liaeul.l'

1

reulaa

..

... oarrt••

••t

at

t •

teapora tare for abou�

1

houro

._..,., whS.o� t1..

\•e

aod1

o

lor14o

oo oeatratioa la

•

ii�tr� roeo

••

a

ut

1 . J

Molar . The e luted proteia aolutioa wu aont••• a•

80

aM bJ paaaSaa thl"oqll a Unlou

(SP '00

aeriea

i)

apeotr • pkotoae ter eqvippe with four t low-thro ab oelle of •ar11Dc

patlalerastb ( , . a .

'

arad

10

-

)

. t'lt.e hotaeeter ....

pi'

,...

to

reacl e••r1

'

•laute• and wu oo plecl to a s.,.

..

. , reoar er ( •o4•1

Slta )

••t to reoord , optloal 4ea•lt1ea betw

..

a

0 ucl

1 .

_Tbe

_{e l}

_u

te thea paaeed to a Glla o •• uea tial lrae tua

oolleotol' , •• • t aolleot ' •1 ••• lee . 'l'h1a proae4u"

as alae '

u...

aatl

aa eYent ••rker ldeatitied the poiDta at whio1t. the traoti n oolleotor •o•e4 the \u'b•• •

!he liquid ooatainiag the fractloaate protein waa �•ll••

ta•o i te trao U.ona and chlorotor• .

..

eel

..

preee:r'latt•• •

ao trao tlo wae 41alraed at

�0

tor ) d.,a , aaaiaat tou�

ob&a�•• t diatt lle4 water, to re•o•e • fter. file fra tt aa

at

this

etace had a Tolu•e ot abo t 20 -

�

ala (4epea iDC

tlle alae ot the oriciaal

eak ) ucl

••n eoaoenvaw '- altos\

' •1• b7 plaolq tn. o loeect

clial.Jaia

baa into a aaall ju , aprlokliac polJ• thJleae 1l7ool oYer i t , o loeiaa the jar , aad boldtac a t �0 tor abou•

1 �

houra . late� ••• •�••• o�t ot the dialJaia

aa

4uriac thla pe rio4 an4 tbe e o oea,rated ••••rtal,

preaerYe4 with ohlo�otor. , wae e l4

la 1

oa. M Cartae7 ••••1••

..

..

..

At a oaYenteat tt•• ( al••7• w ithlD

1

week ) fraetio .. were eeaaae4 o•er

\�e

wa'lelenltb range

2'0

•

)20

aM lA a e o-... D

reeor« iaa apectrophoto•• t•r to lnd toate puri\7 of \be trao

ioaa

an4 to 1n41oate which f�ao tl oaa eo '•1ao4 protein. rra• ttoaa were thereafter he14 at

,

.

ia

the preaoaoe ot e�lorotora for e leotrophore ,1o iaYee tigatloa.

pr tolaa •••• not adoquato lJ 4oaorbod at • _{1¥• o'l}

ri

•

eo

eo

trat lo of

O. }

Molar

w hich 1 _{• ttotac to•y}

t

_o .. eia aad oo aequeatlJ the

•

of

a

e 41

chloride

sradieat

r

e

to

1.2

M wu 1ntro uoe• .

IYen

at tl\ia level t!l re ap eared to be

pr te1n

a till

edaor

to

the

e l luloee.

baerYaiion auat 1 4 1oate that

the

ma ter1 ale , as a ola 1 11ae4 , are

au

aore hi lr aosa iYelf oharged ban the aatt•• .... ,

at pH

1. 0 (

•• _se

ot

1 n

1 0 . 4

tor

further

ta,uaaloa ot

tilia pout > .

Vrea

tor

uee ia _thie prooe d ure ia au-jecte4 t o

a

eioalaatloa Ot:AE.

atop b7 p .. eias thro h a o4 o t Aoe ll loee . 4 deioaieati a tep t. aaid to

lap�•••

reeolutioa ot

rote1a ooapoae

te

ncluoe

the

Ulto 11hoo4 of a.rb Jlation

t

�sine 1

OJ&aah

ioaa

( �h paoa , Qord a ,

oawo ll aa4 rarre ll ,

196�).

Conooatratioa

of

�ho trao ti oaate& pl"oteiaa waa

atorial hi

a

the clial.Jaia •abr ane . PolJe thJlelle c l.lool, a W&Jq

llak1

aubat&Ate wit

aoleoular

wolsbt

ot 20, 000,

waa e d . t

la

prlaol ple b • been uaocl

tor concentration ot

eaa,.ee • ao eYideaoe

of

4aaaso

to the ena7 ... ••• •

•

t.4 (Ko a ,

19,91

The

te

ba:Lque

wao

e iap l1f1

b7

retd S. g • 4la1Jae4 pro tein rrao ti

roaaia

.. wltboQt oeal i llt7 of ooat ..

laat1oa.

Re- • of t •

pol,.t.b.,y�fte

llJOOl

-ttel'

I"J'l 8 1

hea'lq

b &a

OYaa

a\

IJQ0r

•••

111 praoti•••·

Preeerwatlo

sgai na t

�. erial aotiOA ... aobie••

bJ

old1

tr etioaa cold and by

a�ditto

of

o orot ra.

olu

•

•

•

teehaiq •• dHcrt ed ooa:atai •o1 atepa

ud

ia

u ualna eo

that oal1 two or thl'ee eaaplee wepe

haa

le• pal"

-

In document Aplicaciones de la expansión de consultas basadas en ontologías de dominio a la búsqueda de objetos de aprendizaje en repositorios (página 72-80)