681
Supplementary Figure 1. Phylogenetic clustering of all cydA-like sequences. cydA sequences were
682
extracted from a taxonomically diverse set of genomes and metagenomes from IMG and aligned using 683
MUSCLE. The multiple sequence alignment was used to infer a phylogenetic tree using RAxML. The 684
RAxML tree topology was similar to that inferred by PhyML. The three families, qOR, OR-C and OR-N 685
are clearly separated, and 15 subfamilies were designated based on the clustering observed and 686
identifiable sequence characteristics. 687
Supplementary Figure 2. Sequence characteristics of qOR4a-cydA. a. An unrooted phylogenetic tree
688
of cydA sequences from archaea including both qOR4a-type cydA and cydA of the qOR1, qOR2 and 689
qOR3 (in red) types was generated using RaxML. qOR4a type cydA have some internal clusters, 690
identified with a shaded box in green, blue and purple. Characteristics unique to the cluster, when 691
identifiable were indicated. For e.g., the presence of the insertion in the proton channel. b. A multiple 692
sequence alignment of the sequences present in the above clusters, the background of each sequence 693
cluster shaded in red, green, blue and purple according to the colors in the tree in a. Conserved residues 694
corresponding to the ligands, proton channel and a few residues expected to participate in proton-coupled 695
electron transfer are marked. Significantly, several qOR4a-type cydA have a lysine substituted for M393 696
(E. coli numbering) in the active site suggesting an alteration of the midpoint potential of heme b558 in
697
those enzymes. 698
Supplementary Figure 3. GFP-tagged cydAA’ from Caldivirga maquilingensis. The presence of
699
cydAA’ during protein purification protocol was verified by looking at elution fractions under UV-light. 700
Three glass vials containing (from leftmost) elution buffer, an elution fraction containing GFP-tagged 701
Murali et al. ISMEJ-21-00087A 31
cydAA’ and a fraction without cydAA’ are compared. The green fluorescence in the cydAA’ containing 702
fraction is easily distinguishable. 703
Supplementary Figure 4. Mass spectrometric identification of subunit I of cydAA’ from Caldivirga
704
maquilingensis. Partially purified cydAA’ was digested with Chymotrypsin and the digested peptides
705
were separated by HPLC and infused into a Thermo LTQ Velos ETD Pro Mass Spectrometer. The mass 706
fragments recovered after MS/MS fragmentation were subject to analysis by Mascot Distiller and Mascot 707
version 2.4. The analysis revealed peptides from subunit I of cydAA’ in the protein preparation with a 708
MASCOT score of 85. 709
Supplementary Figure 5. SDS-PAGE gel electrophoresis of partially purified cydAA’. A. Cell lysate
710
was loaded onto a Ni-NTA column (Lane 1). The flow-through was loaded onto a Q-sepharose column 711
and subject to elution by changing the salt concentration from 0-500 mM NaCl. Three elution peaks 712
(Lanes 2,3,4) which absorbed at 412 nm were pooled, concentrated and diluted to 50 mM NaCl and then 713
loaded onto a DEAE-Sepharose column and subject to elution under a salt gradient from 0-500 mM NaCl. 714
The elution fraction (Lane 6) which absorbed at 412 nm was pooled and concentrated and used to identify 715
electrophoresis patterns. Assays and spectra were obtained with a sample that was subject to a simpler 716
purification protocol – Ni-NTA followed by DEAE-sepharose because the yield was poor from the 3-step 717
purification protocol. Lane 5 was the Precision Plus Dual Color Standard (Bio-Rad). Subunits I and II are 718
similar in size to the subunits from E.coli cytbd (B). B. Electrophoresis patterns of purified E.coli cytbo3 719
and cytbd. 720
Supplementary Figure 6. Sequence characteristics of qOR4b-cydA from Caldivirga maquilingensis
721
(also referred to as cydA’). a. A topological representation of cydA’ using HMMTOP. The amino acids 722
conserved above 90% identity are shaded in black. b. A multiple sequence alignment of sequences from 723
qOR4a-cydA and qOR4b-cydA family. The former sequences are highlighted with the purple background 724
while the latter are highlighted with a gray background. The absence of the proton channel residues E99 725
and E109 is apparent. The ligand to heme d, His19 and the proton channel residue H126 are completely 726
Murali et al. ISMEJ-21-00087A 32
conserved. The ligands to heme b558 (H186 and M393) and other amino acids typically associated with the
727
quinol binding site in helices V-VIII are not well conserved. Two threonines Thr71 and Thr74 in 728
C.maquilingensis which take the place of Leu71 and Glu74 are completely conserved.
729
Supplementary Figure 7. Structural model of subunits I from Geobacillus thermodenitrificans
730
and Caldivirga maquilingensis respectively. The homology model of cydA from Caldivirga
731
maquilingensis was generated using the Swiss PDB viewer and visualized using VMD. The boxed
732
regions reveal more polar residues in Geobacillus, represented by red and blue, while aromatic
733
residues are colored in green.
Figure 1. Diversity of the cytochrome bd oxygen reductase superfamily. The cytochrome bd oxygen reductase superfamily is divided into 3 families based on phylogenetics and structure – qOR, OR-C and OR-N. qOR is defined by the presence of the quinol binding site in subunit I (cydA). OR-C is missing the quinol binding site but has a heme c binding site in subunit I. OR-N is also missing the quinol binding site and is commonly found in operons containing alternative electron donors. Various subfamilies within each family are also shown (Supplementary Figure 1). The operon context and putative protein complex arrangement of each cydA-containing enzyme is also shown with a reference protein accession number and source microorganism. The potential gene duplication events are highlighted in yellow. A legend is also provided to mark the related conserved domains in the same colors and redox co-factors such hemes and iron-sulfur clusters. A more detailed explanation of the figure including a description of the various subunits and characteristics of the families and subfamilies is provided in Supplementary Material.
Figure 2. Phylogeny of quinol-oxidizing cytochrome bd-type oxygen reductases. At least four clades of quinol-oxidizing cytochrome bd-type oxygen reductases could be identified – qOR1, qOR2, qOR-3 and qOR-4a. The long branch within the qOR1 clade (red star) is comprised of sequences missing the proton channel that is conserved in all other quinol-oxidizing cytochrome
bd-type oxygen reductases. Subunit I of cydAA’ is from the qOR4a family. The cytochrome bd-
type oxygen reductases that do not contain the quinol binding site (OR-C, OR-N, and qOR-4b families) were used as the outgroup.
Figure 3. Distribution of cytochrome bd-type oxygen reductases in Archaea. Cytochrome bd-type oxygen reductases are sporatically distributed throughout the Archaea. The qOR4a family (cydAA’) is predominantly found within the Thermoproteales and Desulfurococcales orders of Crenarchaeota.
C OM BO -56- 21 sp001800815
M etal losp haera hakonensi
Hal orubrum hal ophil um
Nit ros ot alea sp0 019203 95
Hal of erax lars enii
Bog-38 sp003170935 MGIIb-N1 sp002505455 UBA8886 s p00 31 93815 Methanoregula sp002508495 Posei do nia sp00 249 4645 Methanofollis liminatan Nitr osopumil us sp002690535
Nit ros opum ilus s p000746685 CG1-02- 32-21 sp00 1872185
Nit ro sote nuis s p00 2499 525
ARS21 sp002686215
NP1466 sp002689985
MGI Ia-L1 sp002729 095 V ulcanisa et a sp 001316245
Sal inar chaeum sp 000403645
Hydr othermarchaeum profundi
Hal or ubrum sp00 1261915
CBA113 4 sp 0030 231 85
MGIIb- O1 sp002685415
Pos eid onia sp 002 722695 Methanococcoides burtonii
Met hanof astidiosum sp001587595
UBA203 sp003194445
Thalassarchaeum sp002495735
1-14-0-10-32-24 sp002763025
GCA-2685855 sp002685855
Cal divir ga maqui ling en si Met hanob act eri um_B lacu
Asg ard GCA 001940645.1
H aladap tat us pauci halo philu
ASMP01 sp000405225
Parvarchaeum acidophilu
Nit ro so ta le a sp9 00 143 675 Iainarchaeum andersonii
Ni tros osp hae ra garg ens i
ARK- 15 sp002878135 MGI Ia -L 3 sp00 273 139 5 MGI Ib -P sp002457195 Ha lovenus sp0030210 15 ARS1309 sp002688015 MGI Ia- K1 s p002707335
Ha lo des ulfur archa eum f o rmi cic um
NZ1 3-MG1 sp003 0562 85
MGIIb-O3 sp002723045 Methanothrix_A harundinacea_D
Hal omi cr obium sp00 3021685
MGIIa-L3 sp002 723 635 Vu lcanisae ta dist ri buta UBA21 3 s p002713 325
MGII a-L 1 sp002496955 Pyr odic tiu m delane yi Methanococcus mar ipaludi s_D
Methanospirillum hungatei _A
MGIIb- O3 sp002725315 JdFR-42 sp002010305 QS-4 -69- 31 sp003021655
UBA493 sp002499505
Nit ros opumilus sp001541925 UBA493 sp002762975
Thermococcus cleftens i
Metha nofastidiosum s p00158757 5
MGIIa- L1 sp 0024 9901 5 Methanothrix_A harundin acea
Pos eidonia sp 0027 14305 The rmoc occus_A sp000430485
U BA8886 s p002731905
Hal obif or ma halot er restri
GCA-2687275 sp002687275
MGII b-O1 sp002502365 MGIIb-P sp00270196 5
Ferr oplas ma ac idar manu
Pyrococcu s furiosu
Th ermofi lum penden
M et hanopyr us sp00 2201895 Hal oar cula ru bri pro mont or ii
MTP4 sp931 0u
MGII a-L2 sp 002502 215 0-14-0-20-30-16 sp002779075
Methanob revi bacter_A thau eri
Methanohal ophilus euhalobiu
UBA489 sp002505585
H aloar cul a ja ponica
UBA160 s p0025 0660 5
SD8 s p001412335 Metha nocaldococc us j anna schii
HAR CE L1 sp00 3058365
CG1-02-31-27 sp002763115
Methano bre vibacter_A orali
Po seido nia sp002 4956 45
MGIIb-N2 sp002702985 Methanona tronarchae um thermo phi lu m
MGIIb-N2 sp002718 215 Halogranum r ubrum Mia14 sp002214165 MGIIa- K1 s p0 025067 05 Ni troso ar cha eu m sp 002737 44 5 H alovenu s sp0030 21285
Ha lof erax deni tr if ic an
Meth anosarcina sp001714 685
MGII a-L2 sp0 02706 065 Meth an operedens nitroreducens_A
Nanopusillus stetteri_A
Nit ro so spha era sp0 0250 184 5
RBG- 16-50-20 sp00176896 5
Methanosarcina sp002499445
MGI Ib-O2 sp002497245 H alor ubr um tr apanicum
Fervidi coccus f ont i Met hanothermococ cus_A okin awen si
Methanoregula sp002502245
MGI Ia-K1 sp002727515
Pyr obaculum neutrophi lum
FEN- 33 sp003135935
B1-Br10-U2g21 sp 0015 64035
Hal olam ina sp002025255
EX4484-205 s p002255045
Nat rialba aegyptia
Asgard GCA 002825535.1 UBA525 sp002498125
MGII a-L1 sp00 2499 705 Halol ami na pel agica_B
Halo ba ct eri um sp 001 48 55 35
Asgard GCA 001940705.1
Met hanotorri s formici cu
Methanolinea sp002509495 MGIIb-N2 sp0 02712285 M GII a-L3 sp 002 6945 85 JGI-OTU-1 sp0 00494 145 UBA233 sp00 2507245 Py r ob ac u lum ca l id if on ti MGIIa- L1 sp816 0u Meth anococcoides methyl uten
MGI Ia-L1 sp00250 2605
Methanoculleus thermohydrogenotrophicum
0-14-0-20-34-12 sp002779065
MGI Ib -N2 sp00250304 5 UBA7939 sp7939u
Met hanos urat us petracar boni
Natri alba chahannaoensi
CG1-02-39-14 sp001872315
UBA 117 sp0 02 494 78 5
Halohas ta sp001563795
Thalassarchaeum sp002698225 Meth anocaldococ cus_A villosu
Nitrosoc osmi cus exaq uar e
JdFR-11 sp002011035
Methanosa rcina acetiv oran
MGIIb-O3 sp001629205 Methanobreviba ct er sp900314 635
MGIIb-O3 sp001629255
UBA114 sp002506335 Hal apr icum sal inum
21-14-0-10-32-9 sp002762915
Archaeoglobus sp002507545 Halogr anum sp002844195
Me than ob acteri um_ D br yantii
PZKD01 sp 003034855
T herm osphaer a aggre gan
MGI Ib-O3 sp002503285 Ha lo ba cteri um sp 000 23 0955
D esulf ur ococcus m ucosu
H al oplanus natan Sal inigr anum r ubrum
MGIIa -L2 s p8574 u
UBA10161 sp10161u
Asgard GCA 001 940755.1
Thermococcus celer
Meth anobrevib acter_ C a rbo riphi lus_A
EX4484-138 sp002255135
Ther mocladium sp00 2508315 Pyrobacul um fer r ir educen Ni tr osopum ilus sal aria
UBA11998 sp11998u
ARS103 sp002688875
Halor ubr um tr opicale
Nitro sot en uis sp0007 23 185
Methanolinea s p11423u GCA- 000496135 sp000496135 Pyrococc us chitonophagu Nitr os opumilus s p003175215 UBA9949 sp1051 6u Methanoregula sp003157895 MGIIb-O2 sp002730775 Thermococcus_A s ibiricu Methanosalsum zhilin ae Methanothrix sp002 505805 UB A9212 sp9293u Halo arcul a m ar ism or tui
VadinC A11 sp00 2498365 Thermococcus sp002214525
Methanos ar cina b arke ri_B Met hano bac ter iu m sp0 024 968 05
GCA-2686855 sp002686855
Methanoculleus sp002497965
Cal dis phaera sp002506 595 ARS23 sp002686195
Thermococcus sp900198835
Archaeoglobus_B profundu
Methanofollis sp002498315
St aphyl other m us m ar inu SCGC- AAA471-B05 sp00 0380705
Me th an obact eriu m f ormi ci c um_A
Me tha nobrevibac ter_B sp9 00314 605
Na tr onomona s ph ar aon i FEN-99 2 sp0031 41855 1-14-0-10-31-34 sp002763225 Cal divir ga sp002506515 Methanococcus volt ae B24-2 s p002490245
Methan obrev iba cte r_A gottsc halkii
Me thanomethylo vorans sp001 89 6725
20-1 4-0-80- 47-9 sp 002779555
Nitr os oarc hae um_ A sp 00 27818 05
Halococcu s s acc har olyticu
GCA-2688095 sp002688095
Ha lo coccus thail and ens i
Met hanotorris igneu
Halogeometr ic um pall idum
MGI Ia -L1 sp9 354u Tc- Br 11 sp001 564285
A ci dianus sp00056 5255
Hal or ubrum aiding ense
UBA233 sp002010925
MGIIb-P sp00 2498455 Hal orubr um persicum
Methanocorpusculum sp001940805
Ni tr os opumi lus s p000746785 Ni tr osopelagi cus sp001627235
Met hanobr ev ibacter _A sp90 0314695
UBA12030 s p10106u
MGI Ia-L1 sp0 02687075
Metal losphaer a sedula
MGIIb-O5 sp002498925 UBA5 7 s p0 02494 565 SM 1- 50 sp00 2506745 MGII b-O3 sp002719615 MGIIb-O1 sp002708385 Met hanosarci na sp0009 69965
Methermic occus shengliensi
Cald isphaer a lagunen si
Thermogymno monas aci dicola
Hal opl anus v escu
MGIIb-O3 sp002720055 SW-7-4 3-1 sp 0030 09795 Methanocalculus sp9912u UBA12515 sp001507955 Sulfol obus_B sp001316085 MGIIa-L 1 s p8725u UBA226 sp002719475 Metha no bac teriu m_D sp00 25 096 65
GCA-2688925 sp002688925
MGIIb-O5 sp002497985 Halogranum amylol yticum
40CM-2-53-6 sp001918745 Methanoculleus thermophilu UBA11716 sp9638u BE-D s p003019595 ZAV -19 sp002877855 MGI Ia -L1 s p002495535 Methano lobus sp002 507205 Ni trosopumilus sp 002788515 Pyrobaculum sp001189275
Aci dil obus sa cch arovor an
Met hanos arc ina sp11020u
MGIIb-O5 sp002499325 Ha loferax m editerranei
ARS1301 sp002686355
Methano breviba cter_A sp9 003209 55
MGIIb-O3 sp002730445
Acid ul iprof undum sp000327505
QS-8- 65- 32 sp 0030 22925
HRBIN0 2 s p002 89839 5
M eth anomet hylophi lus sp001560915
Halococc us morrh uae
MGIIb-O5 sp002501805
Therm oplas ma sp003205235
Halofer ax sulf uri font i
Methanosarc in a horonoben si Me th anos pha era cuniculi
UBA203 sp002503 59 5 Hal ob ac teriu m ji lan ta iens e
S ulfolobus_B sp0024958 45 MGIIb-O5 s p002719635 Thermococcus guaymasensi Methanospirillum stamsii MGIIb-O5 sp002505775 MGIIb- O3 sp002731195 Halopenit us persi cu MGIIa-L1 sp002727485 WOR-SM1-SCG-A sp001723845 GCA-2687795 sp002687795 UBA431 sp002505525
Halo bifo rm a ni tr at ireducen
PL- Br1 0-U2 g16 sp0015639 95
Cunic uliplas ma di vul gatum Ac i diplas ma aeolic um M9B 1D sp002900535 P yr ol obus f um ari i QS-5- 70- 17 s p003 021 975 QS-1- 69-70 sp0 030235 35 Thermococcus profundu
Methanosarcina vac uol ata
UBA141 sp 002495315
Methanoc occus mari paludis_C
Methanobrevibacter_C arbori philu
Natr onor ubrum b angense
UBA184 sp002495185
H alol ami na rubra
Met hanomassil iicocc us sp0025085 45 H aloar cul a am ylol yti ca
Methanolobus psychroph ilu
MGI Ia- K1 sp002507425
Ni trosopumil us c atali na
Nat ri nema eji nor ense
Al tiarchaeum sp002083985
H alof er ax mucosum
Nit rosopumi lus s p000484975
MGIIa- K1 s p0 026 89 565
Thalas sarchaeum betae
Archaeoglobus_A sp002494625 Pose id oni a sp0 0272 8035
V ulcani sae ta sp001316265
MGII a-L1 sp 002495 945 Methanoregula sp002495065
Methanoperedens nitroreducen H alovi vax r uber
EX4484-2 sp002254565
Bog-38 sp003162175
Methanococcus vannielii
Met hanothermobacter marbur gens i
Methanoregul a sp003136075
UBA10107 sp10107u
SW -1 0-68- 16 sp003023485
Methanoculleus sp002499455
Halor ubr um aet hi opi cum
UBA467 sp002503825 Hal ovenus sp001564135
Therm ogl adius cald erae UBA284 sp002499185 E X4484- 217- 1 sp0 02254745 UBA95 sp002499405 JdFR-07 sp0020 10975 SM 1- 50 s p0 01595945 Methanocorpusculum labreanum Meth anocaldococcus sp000025525 MGII a-K1 sp 002 49 818 5 Methanofa stidi osum sp001587715
B26-1 sp001593935
Halol ami na pel agica
ARS1178 sp002688325
QS- 5- 70-15 sp 003 022 005
Me tha nobrevibact er _A woesei
Halofer ax gibbons i i
Me thanoc occus maripa ludis_A
UBA419 s p00 249 44 95 UBA493 sp002687735
Pyrococcus sp000263735
Me thano bac teriu m sp0 004 99765
H alor habdu s ti am atea
Halorubr um cor iense
MGI Ia -L 4 sp0 027 18195
Me thanos arci na sici liae IMC4 sp001742785
Met hanof astidiosum sp001587675
GCA-2688035 sp002688035
Aci dianus copahuensi
H alost agni cola sp000691505
Pos eidonia sp 0026 9452 5 Hal ovenus sp 003022725
UBA10521 sp8915u
Halarch ae um acidi p hi lum
Met hanoperedens sp002487355 UBA419 s p00249 5625 Caldiarchaeu m su bterraneum Ni trosopelagi cus sp002698885 GCA-2688775 sp002688775 UBA509 sp002498845 U BA102 s p002509225
Asg ard GCA 001 563325.1
MGI Ia -K1 sp 002 715 72 5 Met hano sph aera sp0 0250909 5
Halolamina sp000224475 Halor ubr um li polyt icum
MGIIb-O2 sp002499785 CBA1134 s p00 195 059 5
QS- 1-69-70 sp00 302132 5
MGIIb-N1 sp002717835 Ha lom icr obium zhouii
MG II a-K1 sp002706615
N atr onor ubrum tibe tense
Pyro bacul um aerophil um GCA-2686295 sp002686295
Halorubrum ezzem oulense
Methanoregula sp003154335
UBA148 sp002495885
Methanosa rcina sp8098u
Methanocalculus sp002496395
Met hanob acterium_B sp 003 151535
GCA-2688965 sp002688965
Nit ros ope lagic us b re vi Ni tros ot al ea s p90 017 704 5
Methano sar cin a sp002496565
B26-1 sp001593925
E CH- B- 1 s p003019535
Halopi ger xanaduen si
Huberarchaeum crystalense
Acidi anus manzaensi
MGIIb-O5 sp002730095
RumEn- M2 sp002506255 Thermococcus barossii
Haloco ccu s hamelinen si
SG9 sp 0017 61425 Methanosarcina mazei Syntrophoarchaeum caldariu MGI Ia- I s p002 720095 MGIIb-N2 sp002697105 P ic r ophilus os him ae BOG-1369 sp00 3164 815
Halor ubr um kocuri i
RBG -16 -68- 12 sp001800825
Methanocella_A arvoryzae
U BA1 47 sp00249 6385
U BA8886 sp002509165
Nat ro norubrum daqi ngensi
Methanoculleus sp001896715 QH-6- 64- 20 s p003 0212 35 Fen-7 sp003162615 JdFR-21 sp002011165 Hadarchaeum yellowstonense Poseid onia sp0 027 2649 5 Acidi anus sul fi divor an
Met hanomethyl ophilu s sp002503545
CBA11 34 sp003 0217 85 E X4572- 165 sp002254885 EX4484 52 GCA 002254415.1 B24 sp001593865 Pose idoni a sp00 2704515 UBA1 83 sp00 24952 05 ARS1180 sp002688335 NZBF01 sp002687775 QS- 1-69- 70 s p0030 209 65
Nat ronor ubr um sulfidi faci en
UBA10216 sp10216u Meth anolob us sp002508635 U BA28 5 sp0024 95025 JDFR-14 sp00 20110 85 RB G- 16- 68- 12 sp8695u GCA -001856825 sp001856825
Met hanocaldococ cus vulcaniu
UBA12501 sp10192u
1-14-0-10-45-29 sp002778455
4 0CM-2-53-6 s p001918765
MGIIb-O3 sp002718695 Meth anolobus tindari u
Ther m opr ot eus sp002497345
H aloven us ar anensi
UBA153 sp002503705
UBA8471 sp001871535
Archaeoglobus_A sp002011215 Met han obrev ib act er_ A smi thii_A
Thermococcus radiotoleran
AWOC01 s p0004 9418 5 UBA93 sp002792915
Nitros oarchaeum koreensi
UBA55 sp002495465
Met hanot hermobact er sp000828575
N itr os opumilus s p002317795
UBA108 34 sp10834 u
Ferroglobus placidu
MGII b-O3 sp002722735
P yrodi cti um occul tum
S G8- 5 sp001595885 Methanobrevibacter_B sp00 22086 25 Ha lorubrum sp00049 6175 DTU0 08 sp00250 4525 EX4484-121 sp0 02255 105 MGIIb-O3 sp002172185 MGII b-O3 sp002172375 EX4 484-135 sp00225502 5 Nit rosopumil us s p001510275
Haloco ccu s sediminico la
Ni tros opumilus sp002737455
UBA168 sp002507085
Thalassar chaeum sp002507125 Met hanobrev ib act er_A sp 90031 9535
QS- 1- 69 -70 s p00 302 290 5
Methanoculleus sp002501655
Methano regula sp002495055
Ther moplasma volcani um
MGIIa -L4 sp0 024 954 05
Thermoco ccus_A sp001317345
Methanohalophilus ha lophi lu Haloquadratum wal sbyi _B
Thermococcus_B paral vinell ae
Thalassarchaeum sp002505325
M et hanopyr us kand ler i BM511 sp002867475
COMB O- 56- 21 sp00180 0675 Methan obr eviba cte r_ D curv atu
B 1-B r10- E2g 2 sp002156 705
Hal osim ple x car lsbadense
Po sei donia a lphae
MGII b-O1 sp002497025 UBA11998 sp002762785 Methanor egula sp002835 825 GW2011-AR9 sp10194u S M1- 50 s p002900555 U BA102 s p001875425 Pose id onia sp0 025 02295 Hal oc occ us sp 0030 22685 G CA- 002838935 s p 002838935 Archaeoglobus_C veneficu MGIIa- K1 s p12207u UBA11998 sp002686315 U BA16 2 sp002496 425 CSP1- 1 sp 0014 43365 H alorubrum sp0 03287355
Meth anocaldococ cus ferven
Methanomet hylophilus alvu
MGI Ia-L1 sp 0024 9954 5 Tc- Br 11-E 2g1 sp001564255
UBA8941 sp001 774245
Sulf olobu s_A sol fat ar icu Methanobr evibacter _A sp90031786 5 Methanos arcina sp 002505 485 CG08- 08-20-14 sp002762705 Q S- 5- 68-33 sp003023785 Ha lopenit us persicus_A MGIIb-O1 sp002457595 Methanofoll is ethanolicu
Me thanol obus psy chrotoleran
UBA11384 sp11384u
ARS10 sp002690445
MGI Ia- K1 sp 00 271 020 5
MGIIb-O5 sp002506825 Methan osarcina bark eri_A
UBA164 sp0024990 05
MGI Ia-L1 sp002 688825
Methanoculleus bourgensi
Hal opiger _B dje lfi ma ssi li ensi
MGI Ib-N2 sp00250687 5 Hal omi crob ium kat esii
MGI Ib-P s p00272 2595 DG-33 sp001515185 SD8 sp002509485 MGI Ia-K1 sp 00 269 42 45 Asgard GCA 001940725.1 UBA147 sp0 02499085 Met hanob acter ium sp 000 30986 5
UBA9642 sp12459u
Acidi lobu s sp00049 5735 Methanot hermococcus_A aeolicu
Methanoc ulleus marisnigri _B Halogeomet ri c um rufum
UBA10 452 s p002501 855
Met han osph aera sp00 3268 005
N atr ial ba sp0018 61355
MGII a-L 3 sp0024 96845
MGIIa-L1 sp95 13u B25 sp001593855
Me thanothermococcus thermolith otr ophicu
B1-Br10 -U2g 21 sp0 0156 3915 SW- 9-67- 25 sp003022745 MGIIb- O2 sp002498985 Nitro sop el ag ic us s p0 004 84 935 Vulcani saeta sp002496475 Thermococcu s gorgonariu EX4484-31 sp002254805 VadinCA 11 sp002509405 FE N- 65 sp003 153895 Asgard GCA 002825 51 5.1 Halobellus clavatu UBA8480 sp001871495
Ther mof ilum_A uzonense
UB A287 sp002495235 B23 sp00159 3875
Ther mococ cus_C sp 002009975
Vul c anis aet a di s tr ibut a_D Met ha nosp hae ra sp0 0326 607 5
ARS1403 sp002687825
UBA55 sp002687935
MGIIb-P sp0027 24815
PCYB01 sp002762845
SM 1- 50 sp002 496355 Nitr os osp haera ev erglade nsi
MGIIb -O1 sp002 45 7555
MGIIa-K2 sp002719395
Nanopusil lus stetteri
Acd1 sp000389735
Hal oru brum sp00029 6615
Hal ob acter ium sa lin ar um Halof erax mas s il iens i
UBA349 s p002839705
UBA 9653 sp9653u
Methanocorpusculum sp002506085
Hal oar ch aeobius ir anensi
Methanocaldococ cus bathoardes cen
N atr onobact er ium _A t excoconense
GW2011-AR11 sp12027u
Thalassarchaeum sp002503055
MGI Ia-K1 sp002701145 Nit ros otalea dev ana te rr a
Methanothrix_A sp001602645
Archaeoglobus_A sp002011235
PALSA- 136 8 s p003 1355 75
N atr inem a sal aciae
CG- Epi1 s p002497685 UB A8886 s p002731655
A cidi anus br ier le yi
Halov enus sp003 021335
Me t hano bact eri um_C con gol en se
1-14-0-10-34-76 sp002763075
N atr inema pal lid um
Asgard GCA 001940665.1
Met ha nobac te riu m_D ve te r um
WOR-SM1-SCG sp001723835 FEN-987 sp00315295 5 B6 3 s p001593845 Methanos arcina sp000 979455 CBA11 34 sp 003 021 175 Thalassarchaeum sp002501885 MGIIb-O3 sp002506755 Micrarchaeum acidiphilum JGI -00001 06- J15 sp0 00405 685
Nat ri alba mag adii
Q S-1- 68- 20 sp003021085 H aloar cul a hi spanica
Methanome thylovorans holl andi ca
Sul fol obus_A sp001719125
H alost agnic ola ka mekur ae
Meth anomet hylophilus sp 002495325
Hal ofer ax prahov ense
UBA12276 sp8261u Me thanospha era s p90 032 2125 MGIIa-L1 s p002697705 Halor ubrum sp00 1564205 MGI Ia -K1 sp002 709 705 Asgard GCA 003144275.1 Me th anospha era s p003266 105 QS- 1-69-70 sp00 30210 05 SCGC-AAA011-G17 sp000402515 Methanoculleus sp002508705 MGIIb-O3 sp002496485 Geoglobus ahangari
Nitrosopumilus mar itimu
Me tha nos phae ra s tad tma nae
H aloar cula ar gent inensi
Vulcani saeta di st r ibut a_E
JdFR-45 sp002011395
Methanoculleus sp002498065
Methanocella paludicola
Bog-38 sp003158275
0-14-0-20-59-11 sp002763345
Met all osphaer a yello wston ensi
Methanocella conradii
EX4572 -64 sp002254895 Asgard GCA 0 01563335.1
UBA 71 sp0025 04495
GW2011-AR5 sp000806115
Therm op rot eus uz oniensi
Nat ron ococcus j eotgal i
Methanoculleus chikugoensi H aloferax sp001482285
Met hanolobus prof undi
UBA32 8 sp00250690 5
MGII b-O3 sp002457145