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CAPÍTULO IV. INGENIERIA DEL PROYECTO

4.6. SEGURIDAD Y MANTENIMIENTO

The most important issue to be resolved at this point is

the interference by somatic Salmonella phages infecting the host bacterium which occur at much higher levels than expected. It is, therefore, necessary that further research

be conducted to determine the relative numbers of both somatic

Salmonella phages and FRNA coliphages in natural waters. This will require further examination of waters using this same host bacterium as well as the female strain (S. typhimurium

type WG45). The results of these studies should be able to

determine whether somatic Salmonella phages are consistently

found in high levels or if the population indeed contains significant levels of FRNA coliphages.

Additional work should include the investigation of other strains of Salmonella or related enterobacteriaceae which may

be less susceptible to infection by somatic phages. An F- plasmid could then be inserted into this strain and field tested in both natural waters and sewage to examine its sensitivity to infection by both FRNA and somatic phages.

F-specific RNA plaque isolates collected during this study and future samples should also be serotyped according to the method described by Furuse (1978) . This may help

determine whether FRNA coliphages isolated in these waters

are from the serogroups most frequently associated with human

Further improvements in the membrane filter method should continue with the development of a larger filter holder which would would accomodate larger diameter membrane filters. This would enable larger volumes of water to be filtered at faster

rates as well as reduce the effect of the accumulation of

particulates on the membrane surface.

Finally, this method should be used to compare the level of FRNA phages in source waters with the target organisms,

human enteric viruses. Parallel studies of source and treated

waters for the presence of human enteric viruses and F-

specific RNA coliphages should provide the most accurate

evaluation of the usefulness of this method as an indicator

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