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Mean jump height and FT:CT response to match load are displayed in Figure 3.2 and Figure 3.3 with the change in mean from baseline displayed in Table 3.1. When comparing the jump height between the different levels of PlayerLoad™, at 0.5-h and 18-h post there was a likely reduction (10%, ±7%; moderate effect and 7%, ±4%; small effect) in jump height for medium-load. High-load displayed a very likely (large and moderate effects) reduction in jump height (16%, ±8%; large effect and 9%, ±5%; moderate effect) at 0.5-h and 18-h post respectively. At 42-h, the change in jump height

was unclear across all levels of load. Comparing the FT:CT response between levels of

PlayerLoad™ identified high-load had a very likely 12%, ±7% reduction (moderate effect) in FT:CT at 0.5-h post. At 18-h, there was a most likely and likely reduction (moderate effects) in FT:CT in medium- and high-load respectively.

Figure 3.2 Mean Jump height from 27h pre to 90h post of low, medium and high match loads. Mean values are displayed in log scale with the overall between subject SD shown by the bar labelled SDB. The single bars underneath each time-point represent the within subject SD specific to that time-point. Changes exceeding the ES (0.2) with the qualitative descriptor of **likely (75 to <95%), ***very likely (95 to 99.5%) and ****most likely (>99.5%) is displayed. m: meters.

Figure 3.3 Mean FT:CT from 27h pre to 90h post of low, medium and high match loads. Mean values are displayed in log scale with the overall between subject SD shown by the bar labelled SDB. The single bars underneath each time-point represent the within subject SD specific to that time-point. Changes exceeding the ES (0.2) with the qualitative descriptor of **likely (75 to <95%), ***very likely (95 to 99.5%) and ****most likely (>99.5%) is displayed. m: meters.

Table 3.1 The difference in the magnitude of change from baseline Jump Height, Flight Time:Contraction Time (FT:CT) ratio, Cortisol (C), Testosterone (T) and Testosterone:Cortisol (T:C) ratio between levels of match load.

Match Load 0.5h post 18h post 42h post 66h post

Jump Height L 2.4, ±9.1 0.6 ±5.2 -2.6 ±5.3 1.4 ±5.8 M -9.9, ±7.3; ↓** -6.6, ±4.4; ↓** 1.0, ±4.8 -4.5, ±4.8; ↓** H -15.8 ±8.1; ↓*** -8.7 ±5.3; ↓*** 3.0 ±6.9 -1.4 ±6.5 FT:CT L -5.6, ±6.6; ↓* -5.3, ±9.9 -9.6, ±6.7; ↓** -7.8, ±9.9; ↓* M -5.0, ±6.5; ↓* -17.0, ±7.5; ↓**** -3.6, ±6.4; ↓* -9.2, ±8.5; ↓** H -12.4, ±6.6; ↓*** -12.6, ±10.3; ↓** -4.6, ±8.6 2.9, ±13.0 Cortisol [μg.dL-1_{] L 54.6, ±54.4; ↑*** -0.0, ±21.6 -5.7, ±19.9 34.7, ±27.0; ↑***} M 165.4, ±72.7; ↑**** -14.2, ±17.1; ↓* -30.7, ±13.3; ↓*** -1.0, ±18.0 H 101.2, ±62.6; ↑**** 2.9, ±21.8 -18.0, ±16.9; ↓** -8.2, ±17.1 Testosterone [pg.mL-1_{] L 17.0, ±17.8; ↑** 8.3, ±15.2 12.1, ±14.9; ↑** 21.6, ±20.3; ↑**} M 19.5, ±15.0; ↑*** -10.6, ±11.4; ↓** -11.5, ±10.7; ↓** -9.4, ±13.3; ↓* H 17.0, ±16.1; ↑** 5.8, ±14.5 0.8, ±13.1 -5.7, ±14.3 T:C ratio L -27.2, ±20.6; ↓** 6.1, ±18.0 16.5, ±18.0; ↑** -12.4, ±13.4; ↓** M -54.4, ±10.0; ↓**** 5.1, ±16.4 29.0, ±18.4; ↑*** -7.0, ±13.0 H -42.5, ±14.4; ↓**** 3.0, ±17.1 23.2, ±18.6; ↑*** 4.1, ±14.7

Values are presented as % change in mean, ± 90% CI; direction of response: positive ↑ and negative↓. Symbols denote: *possibly, **likely, ***very likely and ****most likely chance of the true effect exceeding a small (0.2) effect size. m: meters; [μg.dL-1]: micrograms per deciliter; [pg.mL-1]: pictogram per milliliter. L: low match load, M: medium match load, H: high match load.

Mean C, T and T:C response to the match load tertiles are displayed in Figure 3.4, Figure 3.5 and Figure 3.6 respectively, with the change in mean from baseline also displayed in Table 3.1. Compared to baseline (90-h post), at 0.5-h post there was a very

likely (moderate effect) increase in C in low-load, most likely increase in medium-load

(very large effect) and most likely (large effect) increase in high-load (range: 55% to 165%). At 18-h, the change in C was unclear compared to baseline (90-h post). Testosterone displayed likely increases in low- and high-load respectively and very

likely increase in medium-load (range: 17% to 20%; moderate effects) at 0.5-h post.

There were varied responses thereafter. Low-load resulted in a likely reduction (moderate effect) in T:C with moderate and high-load resulting in most likely reductions at 0.5-h post (very large and large effects respectively). There were unclear changes in T:C in all load tertiles at 18-h, with small (low-load) and moderate (medium- and high- load) effects of an increased T:C at 42-h.

3.3.1 Magnitude of change from baseline between levels of PlayerLoad™ (AU)

The magnitude of change from baseline between match load tertiles in all variables is presented in Table 3.2. At 0.5-h, there was a likely greater reduction (12%, ±11%; moderate effect) in jump height in medium-load and very likely greater reduction (18%, ±11%; large effect) in high-load compared to low-load. There was a likely (small effect) greater reduction (8%, ±9%) in FT:CT in high-load compared to medium-load at 0.5-h post . Whilst at 18-h, there was a likely greater reduction (12.4%, ±11.8%; moderate effect) in FT:CT in medium-load compared to low-load. There was a very likely

increase (72%, ±77%; moderate effect) in C in medium-load compared to low-load at 0.5-h post. Differences in the T response between load tertiles were variable whilst the differences in T:C were predominantly unclear.

Figure 3.4 Mean cortisol from 27h pre to 90h post of low, medium and high match loads. Mean values are displayed in log scale with the overall between subject SD shown by the bar labelled SDB. The singular bars underneath each time-point represent the within subject SD specific to that time-point. Changes exceeding the ES (0.2) with the qualitative descriptor of **likely (75 to 95%), ***very likely (95 to 99.5%) and ****most likely (>99.5%) are displayed. [µg.dL-1_{]: micrograms} per decilitre.

Figure 3.5 Mean Testosterone response from 27h pre to 90h post of low, medium and high match loads. Mean values are displayed in log scale with the overall between subject SD shown by the bar labelled SDB. The singular bars underneath each time-point represent the within subject SD specific to that time-point. Changes exceeding the ES (0.2) with the qualitative descriptor of **likely (75 to 95%), ***very likely (95 to 99.5%) and ****most likely (>99.5%) are displayed. [pg.mL-1_{]: pictogram per millilitre.}

Figure 3.6 Mean T:C response from 27h pre to 90h post of low, medium and high match loads. Mean values are displayed in log scale with the overall between subject SD shown by the bar labelled SDB. The single bars underneath each time-point represent the within subject SD specific to that time-point. Changes exceeding the ES (0.2) with the qualitative descriptor of likely ** (75 to 95%), very likely *** (95 to 99.5%) and most likely **** (>99.5%) are displayed.

Table 3.2 The difference in magnitude of change between levels of match load on Jump height, Flight Time:Contraction Time (FT:CT), Cortisol (C), Testosterone (T) and Testosterone:Cortisol (T:C).

Match load 0.5 h Post 18 h post 42 h post 66 h Post

Jump Height M-L -12.0%, ±10.7%; ** -7.2%, ±6.7%; ** 3.6%, ±7.6% -5.8%, ±7.1%; ** FT:CT ratio M-L 0.6%, ±9.3% -12.4%,±11.8%; ** 6.6%,±10.0%; ↑* -1.6%, ±13.4% Cortisol [g.dL-1_{] M-L 71.7%, ±76.6%; ↑*** -14.2%, ±24.6% -26.4%, ±20.4%;} _{** -26.5%, ±18.6%;} _** Testosterone [pg.mL-1_{] M-L 2.2%, ±18.5% -17.4%, ±14.2%; ** -21.0%, ±12.7%; *** -25.5%, ±15.0%; ***} T:C M-L -37.4%, ±22.4%; *** -0.9%, ±22.2% 10.7%, ±22.5% 6.2%, ±20.5% Jump Height H-M -6.5%, ±11.8% -2.2%, ±7.2% 2.0%, ±8.4% 3.2%, ±8.5% FT:CT ratio H-M -7.7%, ±8.7%; ** 5.3%, ±15.0% -1.1%, ±10.4% 13.4%, ±17.2%; ↑** Cortisol [g.dL-1_{] H-M -24.2%, ±31.1% 19.9%, ±33.4% 18.3%, ±31.8% -7.3%, ±21.8%} Testosterone [pg.mL-1_{] H-M -2.1%, ±16.1% 18.3%, ±19.8%; ↑** 13.9%, ±17.7%; ↑** 4.1%, ±19.5%} T:C H-M 26.2%, ±41.3% -2.0%, ±21.3% -4.5%, ±18.7% 12.0%, ±20.1% Jump Height H-L -17.8%, ±10.8%; *** -9.3%, ±7.0%; ** 5.7%, ±9.1% -2.8%, ±8.5% FT:CT ratio H-L -7.2%, ±8.7%; * -7.7%, ±14.0% 5.5%, ±11.4% 11.6%, ±18.0% Cortisol[g.dL-1_{] H-L 30.1, ±60.8% 2.9%, ±29.7 -13.0%, ±24.4% -31.9%, ±17.35%;} _*** Testosterone [pg.mL-1_{] H-L 0.0%, ±18.6% -2.3%, ±17.1% -10.1, ±14.7%} -22.5%, ±15.9%; *** T:C H-L -21.0%, ±29.5% -2.9%, ±21.8% 5.7%, ±21.3% 18.9%, ±22.9%; ↑**

Values are presented as % change in mean, ±CI; direction of response: positive ↑ and negative. Symbols denote: * possibly, ** likely, *** very likely and **** most likely chance of the true effect exceeding a small (0.2) effect size. m: meters; [g.dL-1_{]: micrograms per deciliter; [pg.mL}-1_{]: pictogram per milliliter. M-L: medium compared to low}