Personal Information
Name Sebastian Lücker
Date of birth 6 February 1975
Place of birth Munich, Germany
Citizenship German
Education
Aug 2004 – present Doctoral studies of Life Sciences Ecology at the University of Vienna,
Vienna, Austria.
PhD theses title “Exploring the ecology and genomics of nitrite -oxidizing
bacteria,” supervised by Univ.-Ass. Dipl.-Biol. Dr. Holger Daims and
Univ.-Prof. Dipl. Biol. Dr. Michael Wagner, Department of Microbial Ecology.
Jul 2004 Diploma award (Dipl. Biol.) with grade “sehr gut bestanden.”
Exam subjects: Technical Biology, Microbiology, Neurobiology, and Biophysics.
Diploma thesis at the Department of Microbial Ecology, University of Vienna. Thesis title “Entwicklung und Evaluierung 16S rRNS-gerichteter
Oligonukleotidsonden zum spezifischen Nachweis
deltaproteobakterieller Sulfatreduzierer mittels Fluoreszenz in situ Hybridisierung und Erweiterung des dsrAB-Sequenzdatensatzes ,”
supervised by Dipl.-Biol. Dr. Alexander Loy and Univ.-Prof. Mag. Dr. Michael Wagner, Department of Microbial Ecology, University of Vienna, and Prof. Dr. rer. nat. Karl-Heinz Schleifer, Department of Microbiology, Technical University of Munich.
Oct 1998 – Jul 2004 Course of studies: Biology, major Technical Biology, at the Technical
University of Munich, Munich, Germany.
Areas of specialization: Technical Biology, Microbiology, Neurobiology, and Biophysics.
Jun 1996 Secondary-school diploma (Abitur), Lion-Feuchtwanger-Gymnasium,
Graduate Research Assistance
May 2010 – present WWTF project “Multiphasic comparative analysis of key nitrite-oxidizing
bacteria in wastewater treatment plants.”
Apr 2008 – Apr 2010 FWF project “MICDIF – Linking microbial diversity and ecosystem
functions across scales and interfaces.”
Aug2004 – Mar 2008 WWTF project “Genomics and Ecology of Novel Uncultured Nitrite-
Oxidizing Bacteria in Natural and Engineered Surroundings.” Undergraduate Research Assistance
May 2003 – Jul 2004 Bavarian Research Foundation project „Entwicklung eines universellen
DNA-Chips zum Nachweis von Bakterien in komplexem Probenmaterial.“
Teaching
Jul 2004, Feb 2006, 2007, 2008, 2010
Tutor of the „International FISH Course” at the Department of Microbial Ecology, University of Vienna.
Jul 2004, 2005, 2009, Feb 2005, 2006
Tutor of the course (Projektpraktikum) „Fluoreszenz-in-situ-Hybridisierung (FISH) - Identifizierung nicht-kultivierter Mikroorganismen“ at the Department of Microbial Ecology, University of Vienna
Publications
Lebedeva, E. V., S. Off, S. Zumbrägel, M. Kruse, A. Shagzhina, S. Lücker, F. Maixner, A. Lipski, H. Daims and E. Spieck (in preparation). Isolation and characterization of a moderately thermophilic nitrite-oxidizing bacterium from a geothermal spring.
Lücker, S., M. Wagner, F. Maixner, E. Pelletier, H. Koch, B. Vacherie, T. Rattei, J. S. S. Damsté, E.
Spieck, D. Le Paslier and H. Daims (2010). A Nitrospira metagenome illuminates the physiology and evolution of globally important nitrite-oxidizing bacteria. Proc Natl Acad Sci USA 107(30): 13479-13484.
Bayer, C., N. R. Heindl, C. Rinke, S. Lücker, J. A. Ott and S. Bulgheresi (2009). Molecular characterization of the symbionts associated with marine nematodes of the genus Robbea. Environ Microbiol Reports 1(2): 136-144.
Webster, N. S., M. W. Taylor, F. Behnam, S. Lücker, T. Rattei, S. Whalan, M. Horn and M. Wagner (2009). Deep sequencing reveals exceptional diversity and modes of transmission for bacterial sponge symbionts. Environ Microbiol 12(8): 2070–2082.
Maixner, F., M. Wagner, S. Lücker, E. Pelletier, S. Schmitz-Esser, K. Hace, E. Spieck, R. Konrat, D. Le Paslier and H. Daims (2008). Environmental genomics reveals a functional chlorite dismutase in the nitrite-oxidizing bacterium “Candidatus Nitrospira defluvii”. Environ Microb 10(11): 3043- 3056.
Lücker, S., D. Steger, K. U. Kjeldsen, B. J. MacGregor, M. Wagner and A. Loy (2007). Improved
16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization. J Microbiol Meth 69(3): 523-528.
Daims, H., S. Lücker and M. Wagner (2006). daime, a novel image analysis program for microbia l ecology and biofilm research. Environ Microbiol 8(2): 200-213.
Daims, H., F. Maixner, S. Lücker, K. Stoecker, K. Hace and M. Wagner (2006). Ecophysiology and niche differentiation of Nitrospira-like bacteria, the key nitrite oxidizers in wastewater treatment plants. Water Sci Tech 54(1): 21-27.
Loy, A., C. Schulz, S. Lücker, A. Schopfer-Wendels, K. Stoecker, C. Baranyi, A. Lehner and M. Wagner (2005). 16S rRNA Gene-Based Oligonucleotide Microarray for Environmental Monitoring of the Betaproteobacterial Order “Rhodocyclales”. Appl Environ Microbiol 71(3): 1373-1386.
Zverlov, V., M. Klein, S. Lücker, M. W. Friedrich, J. Kellermann, D. A. Stahl, A. Loy and M. Wagner (2005). Lateral gene transfer of dissimilatory (bi)sulfite reductase revisited. J Bacteriol 187(6): 2203-2208.
Book Chapter
Daims, H., S. Lücker, D. Le Paslier and M. Wagner (2010). Diversity, Environmental Genomics, and Ecophysiology of Nitrite-Oxidizing Bacteria. In: Nitrification, B. B. Ward, D. J. Arp and M. G. Klotz (ed.). Washington, DC, ASM Press: in press.
Oral Presentations
Sebastian Lücker, Michael Wagner, and Holger Daims (2010). A Nitrospira metagenome illuminates
the physiology and evolution of globally important nitrite-oxidizing bacteria. IECB Wissenschaftstag, Vienna, Austria, April 2010.
Sebastian Lücker (2009). Introducing FISH. Hands-On Cryo Electron Microscopy and Tissue in-situ
Hybridization course, Vienna, November 2009
Sebastian Lücker, Michael Wagner, and Holger Daims (2009). The complete genome of “Candidatus
Nitrospira defluvii”. 7th International meeting on Novel Techniques in Microbial Ecology (INTIME), Lackenhof, Austria, August 2009.
Sebastian Lücker, Michael Wagner, and Holger Daims (2007). The complete genome of the
with other Nitrospira-like bacteria. 6th International meeting on Novel Techniques in Microbial Ecology (INTIME), Aarhus, Denmark, October 2007.
Sebastian Lücker, Karin Hace, Frank Maixner, Bettina Anneser, Gertrude Wegl, Kilian Stoecker,
Christian Baranyi, Michael Wagner, and Holger Daims (2005). From phylogeny to ecophysiology: Niche differentiation of nitrite-oxidizing Nitrospira-like bacteria. 5th International meeting on Novel Techniques in Microbial Ecology (INTIME), Lunz am See, Austria, July 2005.
Sebastian Lücker, Alexander Loy, and Michael Wagner (2004). 16S rRNA-based oligonucleotide
microarray for the detection of all recognized and yet uncultured members of the order Rhodocyclales. IECB Wissenschaftstag, Vienna, Austria, April 2004.
Poster Presentations
Sebastian Lücker, Christiane Dorninger, Eva Spieck, Michael Wagner, and Holger Daims (2010).
Novel nitrite oxidizers related to “Candidatus Nitrotoga arctica” frequently appear in wastewater
treatment systems. 13th International Symposium on Microbial Ecology (ISME13), Seattle, WA,
USA, August 2010
Holger Daims, Sebastian Lücker, Eva Spieck, Thomas Rattei, Denis Le Paslier, and Michael Wagner (2010). Genomics and functional analyses reveal unexpected features and suggest a complex
evolution of nitrite-oxidizing bacteria. 13th International Symposium on Microbial Ecology
(ISME13), Seattle, WA, USA, August 2010
Sebastian Lücker, Eric Pelletier, Frank Maixner, Karin Hace, Eva Spieck, Denis Le Paslier, Michael
Wagner, and Holger Daims (2008). The complete genome of the uncultured nitrite oxidizer "Candidatus Nitrospira defluvii“ as basis for comparative genomics with other Nitrospira-like bacteria. VAAM Jahrestagung, Frankfurt, Germany, March 2008.
Niels R Heindl, Christoph Bayer, Sebastian Lücker, Jörg A Ott, Silvia Bulgheresi (2008). Detection
of free-living forms of marine nematode symbionts. 12th International Symposium on Microbial
Ecology (ISME12), Cairns, Australia, August 2008.
Sebastian Lücker, Eric Pelletier, Frank Maixner, Karin Hace, Eva Spieck, Denis Le Paslier, Michael
Wagner, and Holger Daims (2007). The complete genome of the uncultured nitrite oxidizer "Candidatus Nitrospira defluvii“ as basis for comparative genomics with other Nitrospira-like bacteria. ProkaGENOMICS 2007, Göttingen, Germany, October 2007.
Sebastian Lücker, Karin Hace, Frank Maixner, Christian Baranyi, Kilian Stoecker, Michael Wagner
and Holger Daims (2006). Molecular screening of chemically diverse habitats reveals a hitherto
unexplored biodiversity of nitrite-oxidizing Nitrospira-like bacteria. 11th International Symposium
on Microbial Ecology (ISME 11), Vienna, Austria, August 2006.
Sebastian Lücker, Frank Maixner, Bettina Anneser, Gertrude Wegl, Kilian Stoecker, Michael
bacteria to shifts of nitrite concentration: an example of niche differentiation in the microbial world. VAAM Jahrestagung, Göttingen, Germany, September 2005.
Karin Hace, Sebastian Lücker, Frank Maixner, Christian Baranyi, Kilian Stoecker, Michael Wagner and Holger Daims (2005). Molecular screening of chemically diverse habitats reveals a hitherto unexplored biodiversity of nitriteoxidizing Nitrospira-like bacteria. VAAM Jahrestagung, Göttingen, Germany, September 2005.
Sebastian Lücker, Alexander Loy, and Michael Wagner (2004). 16S rRNA-based oligonucleotide
microarray for the detection of all recognized and yet uncultured members of the betaproteobacterial order Rhodocyclales. Minisymposium: Microbial ecology: from ecosystem functioning to biotechnology application, Vienna, Austria, March 2004