1.3. PREGUNTAS DIRECTRICES
2.2.13. EVALUACIONES FISIOTERAPEUTICAS
2.2.13.8. TEST POSTURAL
This paper was published as part of a review on the “Analysis of integrin functions in peri-implantation embryos, hematopoietic system, and skin”, and focuses on methods permitting to analyze the functions of integrins in the hematopoietic and the immune system in gene targeted mice. The availability of Cre lines that induce a deletion of loxP-site flanked genes facilitates the analysis of integrin functions in basically all branches and cell types of the hematopoietic system. In addition, hematopoietic system-restricted integrin knockouts can be generated with the help of bone marrow chimeric mice. Subsequently flow cytometric methods that are essential for the examination of the hematopoietic system are discussed. Another interesting feature of hematopoietic cells is the fact that most of them can be readily isolated, cultivated and analyzed in detail in vitro. Therefore, we describe the generation of dendritic cells and methods to analyze the proliferation of T lymphocytes. In the end, we explain the induction of EAE in mice, since this is an elegant system to test the properties of integrin deficient hematopoietic cells in an autoimmune disease model.
8 Acknowledgements
This thesis would not have been possible without the help of many people and at this point I would like to express my sincere gratitude to:
Prof. Dr. Reinhard Fässler, for giving me the opportunity to take part in advanced research in his department, for his continuous support and encouragement and for the fruitful discussions throughout this work.
Dr. Michael Sixt and Prof. Dr. Cord Brakebusch, for supervising this work with never-ending enthusiasm and for many valuable discussions.
Prof. Dr. Markus Sperandio, who agreed to be the second referee of my thesis and Prof. Dr. Martin Biel, Prof. Dr. Karl-Peter Hopfner, Prof. Dr. Angelika Vollmar and PD Dr. Stefan Weiss, the members of my thesis committee for taking the time to review my work.
Prof. Dr. Britta Engelhardt, Dr. Caroline Coisne and Heidi Tardent, for the fruitful and exciting collaboration and also for the warm welcome during my visits to their lab.
The members of the Department of Neuroimmunology at the MPI of Neurobiology, particularly Prof. Dr. Hartmut Wekerle, Dr. Andreas Holz, Dr. Florian Kurschuß, Dr. Wolfgang Klinkert, Dr. Bernadette Pöllinger and Dr. Gurumoorthy Krishnamoorthy. They always had time to discuss with me and give advice and reagents during the sometimes challenging task of setting up the EAE system in our department.
All current and former members of the department, in particular Anika, Gerd, Hao-Ven, Katrin, Ralph, Sara and Siegfried. Thanks for your friendship, for creating a good working atmosphere and especially for the discussions and the fun during lunch and coffee breaks. Special thanks go to my three bench mates, Tatjana, Ola and Tim – it was always a pleasure to work next to you!
All the people that provided the “infrastructure” for this thesis, especially Dr. Walter Göhring, Sylvia Zehner and the animal care takers, particularly Diana Schmidt.
In the end I would like to thank my parents, Stephanie and Wolfram, for their support and confidence. And I owe great thanks to Frank, for his understanding, love and encouragement, for many discussions about science, but especially for reminding me sometimes that there is more to life than science!
9 Curriculum vitae
Martina Bauer
Address
Bellinzonastr. 5 81475 Munich
Date and Place of Birth
02.06.1980, Eutin, Germany
Education and Scientific Experience
07/2004 – present Max Planck Institute of Biochemistry Munich, Germany
Ph.D. student in the Department of Molecular Medicine headed by Prof. Dr. Reinhard Fässler
11/2003 – 05/2004 Friedrich-Alexander University Erlangen-Nuremberg
Erlangen, Germany
Diploma thesis in the group of Dr. Viktor Wixler at the Nikolaus-Fiebiger- Centre for Molecular Medicine. Title: “TES – ein neuer Interaktions- partner der Integrin α7A-Isoform”
10/2002 – 01/2003 Netherlands Cancer Institute Amsterdam, The Netherlands
Research training in the group of Prof. Dr. Arnoud Sonnenberg at the Department of Cell Biology
11/1999 – 10/2003 Friedrich-Alexander University Erlangen-Nuremberg
Erlangen, Germany
Study of Molecular Medicine. Major subject: Cell Biology. Minor subjects: Immunology, Pharmacology and Human Genetics
10/1990 – 09/1999 Leibniz-Gymnasium Altdorf Altdorf, Germany
Secondary school, general qualification for university entrance
10/1986 – 09/1990 Grundschule Schwarzenbruck Schwarzenbruck, Germany
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