Tipos de aerosoles - Fundamentos b´ asicos sobre aerosoles

2. Fundamentos b´ asicos sobre aerosoles

2.2. Tipos de aerosoles

-R as Ex on 1 K -R as Ex on 2 N-R as Ex on 2

Summary of Genes Included in Each Individual MPCR Kit

Human

MP-70126 + + +

Quality control validation of MPCR kit with individual primer pair and multiplex primer set,, MP-70126

Lane N: PCR usingHuman Ras MPCR without positive (negative) Lane 1: PCR usingHuman Ras MPCR with 1X positive

Lane 2: PCR usingHuman K-Ras exon 1 PCR with 1X positive Lane 3: PCR usingHuman K-Ras exon 2 PCR with 1X positive Lane 4: PCR usingHuman N-Ras exon 2 PCR with 1X positive Lane M: DNA M.W. Marker

For detailed ordering information, please refer to the page containing ordering information at the end of each section.

N M 1 2 3 4

M-PCR

upregulated is not yet understood, but in some cases, elevated expression may be caused by an increase in the level of c-Myc.

For detailed ordering information, please refer to the page containing ordering information at the end of each section.

Summary of Genes Included in Each Individual MPCR Kit

18S c-myc TER TERT TP-1 TRF-1 TRF-2

Human

MP-70140 + + + + + + +

Quality control validation of MPCR kit with individual primer pair and multiplex primer set, MP-70140

Lane N: PCR using hTELS Primers without positive (Negative) Lane 1: PCR using hTELS Primers with 1X positive

Lane 2: PCR using Human H18S Primers with 1X positive Lane 3: PCR using Human TRF-1 Primers with 1X positive Lane 4: PCR using Human c-Myc Primers with 1X positive Lane 5: PCR using Human TRF-2 Primers with 1X positive Lane 6: PCR using Human TP-1 Primers with 1X positive Lane 7: PCR using Human TERT Primers with 1X positive Lane 8: PCR using Human TER Primers with 1X positive Lane M: DNA M.W. Marker

N M 1 2 3 4 5 6 7 8

Human

Cat. No. Product Description Rxns.

MP-70140 MPCR Kit for Human Telomerase Genes, with H18S as Internal Control 50 MP-70139 MPCR Kit for Human Telomerase Genes, with H18S as Internal Control 100

Order Information

89

cDNA Quality Control Validation of Telomerase Associated MPCR Kit, MP-70140

Lane N: Negative control Lane M: DNA M.W. Marker

Lane 1: Positive control of Telomerase Associated MPCR Kit Lane 2-7: 6 different human patient’s brain tissue tested by Telomerase Associated MPCR Kit

Order Information

Human

Cat. No. Product Description Rxns.

MP-70180 MPCR Kit or Human Thrombosis Point Mutations Detection 50 MP-70179 MPCR Kit or Human Thrombosis Point Mutations Detection 100 increased risk of venous thrombosis.

Maxim has developed a multiplex PCR amplification kit to detect site-directed mutagenesis for one-step determination of Factor V Linden and G20210A presence in the prothrombin gene.

For detailed ordering information, please refer to the page containing ordering information at the end of each section.

Genomic DNA quality control validation of human thrombosis MPCR Kit M: DNA M.W. Marker

lane 1: PCR using wild type MPCR primers with patient #1 DNA lane 2: PCR using mutant type MPCR primers with patient #1 DNA lane 3: PCR using wild type MPCR primers with patient #2 DNA lane 4: PCR using mutant type MPCR primers with patient #2 DNA lane 5: PCR using wild type MPCR primers with patient #3 DNA lane 6: PCR using mutant type MPCR primers with patient #3 DNA lane 7: PCR using wild type MPCR primers with patient #4 DNA lane 8: PCR using mutant type MPCR primers with patient #4 DNA lane 9: PCR using wild type MPCR primers with wild type positive control lane 10: PCR using mutant type MPCR primers with mutant type positive control

90 Summary of Genes Included in Each Individual MPCR Kit

Human

MP-70180 + + +

Facto V Factor IX Prothrombin

Prothrombin Factor IX Factor V M 1 2 3 4 5 6 7 8 9 10

M-PCR

18S Beta-Actin L-32 PhosphoLipaseA2 Transferin Receptor GAPDH

Human

MP-70076

+

-

+

MP-70077

+

-

+

+ + +

Mouse

MP-70218

+

-

- - +

For detailed ordering information, please refer to the page containing ordering information at the end of each section.

Human

Cat. No. Product Description Rxns.

MP-70076 Human House-Keeping Genes MPCR, Set 1 50

MP-70074 Human House-Keeping Genes MPCR, Set 1 100

MP-70077 Human House-Keeping Genes MPCR, Set 2 50

MP-70075 Human House-Keeping Genes MPCR, Set 2 100

Mouse

Cat. No. Product Description Rxns.

MP-70218 Mouse House-Keeping Genes MPCR, Set 1 50

MP-70217 Mouse House-Keeping Genes MPCR, Set 1 100

91 Order Information

Quality control validation of MPCR kit with individual primer pair and multiplex primer set, MP-70218.

Lane N: PCR using hHKG Primers without positive (Negative) Lane 1: PCR using mHKG Primers with 1X positive

Lane 2: PCR using Mouse GAPDH Primers with 1X positive Lane 3: PCR using Mouse 18S Primers with 1X positive Lane 4: PCR using Mouse Beta-Actin Primers with 1X positive Lane 5: PCR using mHKG Primers with mouse cDNA Lane 6: PCR using Mouse GAPDH Primers with mouse cDNA Lane 7: PCR using Mouse 18S Primers with mouse cDNA Lane 8: PCR using Mouse Beta-Actin Primers with mouse cDNA Lane M: DNA M.W. Marker

N M 1 2 3 4 5 6 7 8

cDNA are prepared from various sample tissues and tested with MPCR kit,MP-70076

Lane 1: PCR using hHKG Primers with 0.025 mg Human T-cell cDNA. Lane 2: PCR using hHKG Primers with 0.05 mg Human T-cell cDNA. Lane 3: PCR using hHKG Primers with 0.1 mg Human T-cell cDNA. Lane 4: PCR using hHKG Primers with 0.2 mg Human T-cell cDNA Lane 5: PCR using hHKG Primers with 0.1 mg Human T-cell cDNA + PHA Treatment

Lane 6: PCR using hHKG Primers with 0.2 mg Human T-cell cDNA + PHA Treatment

Lane N:PCR using hHKG Primers without positive (Negative) Lane 7: PCR using hHKG Primers with 1X positive

Lane 8: PCR using Human H18S Primers Lane 9: PCR using Human GAPDH Primers Lane 10: PCR using Human Beta-Actin Primers Lane M: DNA M.W. Marker

Summary of Genes Included in Each Individual MPCR Kit

16S Cag A Flagellin Urea C

This Kit is designed to rapidly detect Helicobacter pylori using multiplex PCR. A segment of following genes in H. pylori genome: CagA, UreaC, Flagellin and 16S rRNA will be co-amplified in a single tube. The PCR primers have similar Tm and no obvious 3'-end overlap to enhance multiple genes amplifications. The kit will yield the 348 bp (Cag A), 315 bp (UreaC), 152 bp (Flagellin), and the 110 bp (16S) PCR products when using the positive control DNA provided in the Kit. PCR products can be distinguished on a regular agarose gel.

For detailed ordering information, please refer to the page containing ordering information at the end of each section. MP-70081 + + + +

Bacteria

Cat. No. Product Description Rxns.

MP-70081 MPCR Kit for H. pylori Detection, 50 Rxn. 50 MP-70080 MPCR Kit for H. pylori Detection, 100 Rxn. 100

Quality control validation of MPCR kit with individual primer pair and multiplex primer set, MP-70081.

Lane N: PCR using HPY MPCR Primers without positive (Negative) Lane 1: PCR using HPY MPCR Primers with positive control Lane 2: PCR using Cag A Primers with positive control Lane 3: PCR using Urea C Primers with positive control Lane 4: PCR using Flagellin Primers with positive control Lane 5: PCR using 16S Primers with positive control Lane M: DNA M.W. Marker

92

N M 1 2 3 4 5

M-PCR

Summary of Genes Included in Each Individual MPCR Kit

env

HIV type 1 GAG HIV type 2 GAG p

MP-70073 + + + +

Virus

Cat. No. Product Description Rxns.

MP-70073 MPCR Kit For HIV Type I/II 50

MP-70072 MPCR Kit For HIV Type I/II 100

Order Information

Multiplex PCR of HIV-I & II under MPCR Buffers 1-5 (lanes 1-5). CE analysis of HIV MPCR amplicons (lane 2) generated using Maxim's MPCR Optimizer System.

1 2 3 4 5 M

For detailed ordering information, please refer to the page containing ordering information at the end of each section.

Summary of Genes Included in Each Individual MPCR Kit

has revealed that the E6 protein encoded by the HPV “high risk” types prevalent in cancers forms a tripartite complex with the p53 tumor suppressor protein and a cellular protein termed E6-AP, resulting in the degradation of p53. The E7 protein encoded by “high- risk” HPV types shows high-affinity association with the retinoblastoma tumor suppressor, pRb.

94

MP-70079 + + + + - + _ _ MP-70216 + + + + + + + + HPV -6 HPV -1 1 HPV -16 HPV -18 HPV -31 HPV -33 HPV -52 HPV -58

Quality Control Validation of Human Parpillomavirus Subtypes MPCR Kit, MP-70079

Lane C: Caski cell extract tested by HPV MPCR Kit Lane H: Hela cell extract tested by HPV MPCR Kit Lane N: Negative control

Lane M: M.W. Marker

Lane 1: PCR using HPV MPCR with 1X positive Lane 2: PCR using HPV-16 PCR with 1X positive Lane 3: PCR using HPV-33 PCR with 1X positive Lane 4: PCR using HPV-18 PCR with 1X positive Lane 5: PCR using HPV-6 PCR with 1X positive Lane 6: PCR using HPV-11 PCR with 1X positive

C H N M 1 2 3 4 5 6

N M 1 2 3 4 5 6 7 8 9

Quality Control Validation of Human Parpillomavirus subtypes MPCR Kit,MP-70216

Lane N: PCR using HPV MPCR Primers without positive (Negative). Lane M: DNA M.W. Marker

Lane 1: PCR using HPV MPCR Primers with 1X positive Lane 2: PCR using HPV-16 Primers with 1X positive Lane 3: PCR using HPV-18 Primers with 1X positive Lane 4: PCR using HPV-33 Primers with 1X positive Lane 5: PCR using HPV-31 Primers with 1X positive Lane 6: PCR using HPV-58 Primers with 1X positive Lane 7: PCR using HPV-11 Primers with 1X positive Lane 8: PCR using HPV-6 Primers with 1X positive Lane 9: PCR using HPV-52 Primers with 1X positive

Virus

Cat. No. Product Description Rxns.

MP-70079 MPCR Kit for Human Papillomavirus 50

MP-70078 MPCR Kit for Human Papillomavirus 100

MP-70216 MPCR Kit for Human Papillomavirus Set 2 50 MP-70215 MPCR Kit for Human Papillomavirus Set 2 100

M-PCR

Bacteria

Cat. No. Product Description Rxns.

MP-70214 MPCR Kit for Atypical Pneumonias Diseases, PCP/MPN/LPN/ NUM 50 MP-70213 MPCR Kit for Atypical Pneumonias Diseases, PCP/MPN/LPN/ NUM 100

Order Information

For detailed ordering information, please refer to the page containing ordering information at the end of each section.

C. pneumoniae L. pneumophila M. pneumoniae P. carini

MP-70214 + + + +

Quality Control Validation of Human Parpillomavirus MPCR Kit, MP-70214

Lane N: PCR using PCP MPCR Primers without positive control (Negative) Lane 1: PCR using PCP MPCR Primers with 1X positive control

Lane 2: PCR using NUM MPCR Primers with 1X positive control Lane 3: PCR using MPN MPCR Primers with 1X positive control Lane 4: PCR using PCP MPCR Primers with 1X positive control Lane 5: PCR using LPN Primers with 1X positive control Lane M: DNA M.W. Marker

NUM (871 bp) MPN (375 bp) PCP (300 bp) LPN (232 bp) M N 1 2 3 4 5

95 Summary of Genes Included in Each Individual MPCR Kit

MP-70120 + + +

C. trachomatis cryptic plasmid N. Gonorrhoeae cppB U. urealyticum Urease

(UU) PCR products.

Bacteria

Cat. No. Product Description Rxns.

MP-70120 MPCR Kit for Sexual Transmitted Diseases, CTR/UU/NG 50 MP-70119 MPCR Kit for Sexual Transmitted Diseases CTR/UU/NG 100

Order Information

For detailed ordering information, please refer to the page containing ordering information at the end of each section.

Quality control validation of MPCR kit with individual primer pair and multiplex primer set, MP-70120

Lane N:PCR using STD MPCR Primers without positive control (Negative)

Lane M: DNA M.W. Marker

Lane 1: PCR using STD MPCR with 1X positive control Lane 2: PCR using C. trachomatis with 1X positive control Lane 3: PCR using U. urealyticum with 1X positive control Lane 4: PCR using N. Gonorrhoeae with 1X positive control

N M 1 2 3 4

M-PCR

Summary of Genes Included in Each Individual MPCR Kit

Adenovirus Coronavirus Influenza Virus Influenza A Influenza B Parainfluenza 1 Parainfluenza 2 Parainfluenza 3 Rhinovirus RSV

Human

MP-70178 + + + - - - + +

MP-70203 + - - + + + + + - +

Order Information

Human

Cat. No. Product Description Rxns.

MP-70178 MPCR Kit for Respiratory Infection Associated Virus Set 2 50 MP-70177 MPCR Kit for Respiratory Infection Associated Virus Set 2 100 MP-70203 MPCR Kit for Respiratory Infection Associated Virus Set 3 50 MP-70202 MPCR Kit for Respiratory Infection Associated Virus Set 3 100 For detailed ordering information, please refer to the page containing ordering information at the end of each section.

Quality control validation of MPCR kit with individual primer pair and multiplex primer set, MP-70178

Lane N: PCR using VIR2 MPCR Primers without positive (Negative) Lane M: DNA M.W. Marker

Lane 1: PCR using VIR2 MPCR Primers with 1X positive Lane 2: PCR using Human Rinho virus Primers Lane 3: PCR using Human Adenovirus Primers Lane 4: PCR using Human Influenza virus Primers Lane 5: PCR using Human Corona virus Primers Lane 6: PCR using Human RSV Primers

N M 1 2 3 4 5 6

Quality control validation of MPCR kit with individual primer pair and multiplex primer set, MP-70203

Lane N: PCR using VIR-M053 Primers without positive (Negative) Lane M: DNA M.W. Marker

Lane 1: PCR using VIR3 Primers with 1X positive

Lane 2: PCR using Parainfluenza virus type 1 Primers with 1X positive Lane 3: PCR using Adenovirus Primers with 1X positive

Lane 4: PCR using Parainfluenza virus type 2 Primers with 1X positive Lane 5: PCR using Influenza virus type B Primers with 1X positive Lane 6: PCR using Parainfluenza virus type 3 Primers with 1X positive Lane 7: PCR using RSV Primers with 1X positive

Lane 8: PCR using Influenza virus type A Primers with 1X positive N M 1 2 3 4 5 6 7 8

For detailed ordering information, please refer to the page containing ordering information at the end of each section.

18S MMP-1 MMP-2 MMP-3 MMP-7 MMP-9 MMP-13 MT

-MMP1

TIMP-1 TIMP-2 TIMP3 TIMP4 Tryptase GAPDH

Human MP-70109 - - - + + - + + - - - - + + MP-70110 + - - + + - + + - - - - + - MP-70111 - + - - - + + - - - - + MP-70186 + + - - - + + - - - - - MP-70184 - + + + + + - - - + MP-70222 - - + - - + - - + + + + - +

Quality control validation of MPCR kit with individual primer pair and multiplex primer set, MP-70222

Lane N: PCR using Negative control

Lane 1: PCR using hMMP4G Primer with 1X Positive Lane 2: PCR using Human GAPDH Primers with 1X Positive Lane 3: PCR using Human MMP9 Primers with 1X Positive Lane 4: PCR using Human TIMP3 Primers with 1X Positive Lane 5: PCR using Human TIMP4 Primers with 1X Positive Lane 6: PCR using Human TIMP1 Primers with 1X Positive Lane 7: PCR using Human MMP2 Primers with 1X Positive Lane 7: PCR using Human TIMP2 Primers with 1X Positive Lane M: DNA M.W. Marker

N M 1 2 3 4 5 6 7

Quality control validation of MPCR kit with individual primer pair and multiplex primer set, MP-70109

Lane 1: PCR using hMMP1G Primer with 1X Positive Lane 2: PCR using Human GAPDH Primers with 1X Positive Lane 3: PCR using Human MMP13 Primers with 1X Positive Lane 4: PCR using Human MMP3 Primers with 1X Positive Lane 5: PCR using Human MMP7 Primers with 1X Positive Lane 6: PCR using Human MMP1 Primers with 1X Positive Lane 7: PCR using Human Trytase Primers with 1X Positive Lane M: DNA M.W. Marker

N M 1 2 3 4 5 6 7

Order Information

Human

Cat. No. Product Description Rxns.

MP-70109 MPCR Kit For Human Matrix, MMP Genes, with GAPDH as Internal Control 5 0 MP-70106 MPCR Kit For Human Matrix, MMP Genes, with GAPDH as Internal Control 1 0 0 MP-70110 MPCR Kit For Human Matrix, MMP Genes, with H18S as Internal Control 5 0 MP-70107 MPCR Kit For Human Matrix, MMP Genes, with H18S as Internal Control 1 0 0 MP-70111 MPCR Kit For Human MMP Genes Set-2, with GAPDH as Internal Control 5 0 MP-70108 MPCR Kit For Human MMP Genes Set-2, with GAPDH as Internal Control 1 0 0 MP-70186 MPCR Kit For Human MMP Genes Set-2, with 18S as Internal Control 5 0 MP-70185 MPCR Kit For Human MMP Genes Set-2, with 18S as Internal Control 1 0 0 MP-70184 MPCR Kit For Human MMP Genes Set-3, with GAPDH as Internal Control 5 0 MP-70183 MPCR Kit For Human MMP Genes Set-3, with GAPDH as Internal Control 1 0 0 MP-70222 MPCR Kit For Human MMP Genes Set-4, with GAPDH as Internal Control 5 0 MP-70221 MPCR Kit For Human MMP Genes Set-4, with GAPDH as Internal Control 1 0 0

M-PCR

growth, viability, DNA, RNA and protein synthesis, morphology and virus propagation etc. Thus, mycoplasma contamination in cell cultures may lead to unreliable experiments and develop unsafe biological products. Testing for mycoplasma is a necessary quality control to assure accurate research and reliable biotechnological products.

Several different approaches have been used to detect mycoplasmas in cell cultures, including microbiological culture, DNA fluorescent staining, ELISA, immunofluorescence detection and electron-microscopy. Although each of these tests has its own specific advantages, none offer the combined sensitivity, specificity and speed associated with the use of PCR to detect mycoplasma. We have developed a PCR assay to detect a specific fragment of mycoplasma 16S rRNA gene using nested PCR. This kit provides a simple, fast method to screen for Mycoplasma positives with high reliability.

√

-detect >95% mycoplasma

√

High Specificity

√

Species Identification

-identify species using enzyme digestion

√

Speedy and Easy-to-use

-obtain clear result in less than 1 day

Order Information

Cat. No. Product Description Rxns.

MP-70114 Mycoplasma PCR detection kit 100

Kit Components

2X Optimal MPCR Buffer 10 pairs mixed PCR Primers Positive control

ddH₂O (DNase free) 100 bp DNA ladder Instruction Manual

Lane N: MPL Primer without Positive Control (Negative)

Lane 1: MPL-P001 with 106_{copies/positive control}

Lane 2: MPL-P001 with 105_{copies/positive control}

Lane 3: MPL-P001 with 104_{copies/positive control}

Lane 4: MPL-P001 with 102_{copies/positive control}

Lane 5: MPL-P001 with 50 ng of M. genitalium genomic DNA (G37 Strain) Lane 6: MPL-P001 with 50 ng of M. pneumoniae genomic DNA (B10 Strain) Lane 7: MPL-P001 with 50 ng of M. fermentens genomic DNA (K7 Strain) Lane 8: MPL-P001 with 50 ng of M. hominis genomic DNA (common strain) Lane 9: MPL-P001 with 50 ng of M. penetrans genomic DNA (GTU-54 Strain) Lane 10: MPL-P001 with 50 ng of M. Sualir genomic DNA

Lane M: DNA M.W. Marker

{If the sample contains a high copy number, there may be two amplicon sizes (520 & 320 bp)}

N M 1 2 3 4 5 6 7 8 9 10

520 bp 320 bp

Human

Cat. No. Product Description Rxns.

MP-70116 MPCR Kit For Human NO Metabolism Genes, with GAPDH as Internal Control 50 MP-70115 MPCR Kit For Human NO Metabolism Genes, with GAPDH as Internal Control 100

Mouse

Cat. No. Product Description Rxns.

MP-70118 MPCR Kit For Mouse NO Metabolism Genes, with GAPDH as Internal Control 50 MP-70117 MPCR Kit For Mouse NO Metabolism Genes, with GAPDH as Internal Control 100

Rat

Cat. No. Product Description Rxns.

MP-70063 MPCR Kit For Rat NO Metabolism Genes, with GAPDH as Internal Control 50 MP-70062 MPCR Kit For Rat NO Metabolism Genes, with GAPDH as Internal Control 100

IL1-beta eNOS iNOS nNOS TNF-alpha GAPDH

Human MP-70116 + + + + + + Mouse MP-70118 + + + + - + Rat MP-70063 - + + + - +

Summary of Genes Included in Each Individual MPCR Kit

For detailed ordering information, please refer to the page containing ordering information at the end of each section.

Quality control validation of MPCR kit with individual primer pair and multiplex primer set, MP-70116

Lane N: PCR using hNOSG Primers without positive (Negative) Lane 1: PCR using hNOSG Primers with 1X positive

Lane 2: PCR using Human GAPDH Primers Lane 3: PCR using Human TNF-alpha Primers Lane 4: PCR using Human IL1-beta Primers Lane 5: PCR using Human eNOS Primers Lane 6: PCR using Human nNOS Primers Lane 7: PCR using Human iNOS Primers Lane M: DNA M.W. Marker

In document Estudio de los aerosoles en columna sobre Cáceres a partir de medidas radiométricas (página 32-37)