Anexo N° 14 Distribución de planta
B. Requisitos del puesto a Habilidad.
1.3 Trabajo de otros.
This Appendix includes the participant instructions in the study discussed in Chapter 6 that titled, “Assessment of Human Exposure to Polycyclic Aromatic Hydrocarbons Employing Personal Air Sampler and Urinary PAH Biomonitoring”.
Instructions for Study Participation
Total study duration: 2 weeks (Monday through Sunday)
Sampling days: 4 days/week, i.e. Tuesday, Wednesday, Saturday and Sunday. In total, there will be 8 sampling days in this study (Table A.1). Non sampling days: Monday, Thursday and Friday. In total, there will be 6 non-sampling days in this study.
Table A.1 Study design for the personal air sampling and biomonitoring study
No. Week Day Driving PAH-containing food
1 Week 1 Tuesday-Wednesday High Low
2 Week 1 Saturday-Sunday Low Low
3 Week 2 Tuesday-Wednesday High High
4 Week 2 Saturday-Sunday Low High
During the 8-day sample collection period
• Collect a ~60 mL urine sample into urine cups every time you urinate. Estimate and record the total volume of urine excreted each time.
• Carry a portable air pump connected to an air sampling tube at all time. Change the sampling tubes four times a day, and record the sampling duration and time of change.
• Complete the Daily Activity Log (Log). Note on the Table at what time you visited the restroom and the total volume of urine excreted, time of air sampler change, duration of air sampling, as well as driving and dietary information.
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Every Wednesday and Thursday morning, we will collect your coolers withsamples and any waste items, and give you 2 other coolers (one for air samplers and one for urine samples) with fresh cold packs and one-day supplies. For the weekends, we will give you two-day supplies on Friday afternoon, collect samples and waste items on Monday morning.
During the 6 non-sampling days
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No urine and air samples will be collected• We will not provide food. But please avoid BBQ, grilled, smoked food, and fast- food hamburger. If you eat those foods, please note the type and amount of food, and time of the intake on the questionnaire sheet.
Urine Sampling Instruction
Instruction for urine sample collection, transport and storage 1. Collect all urine excreted in a disposable graduated beaker 2. Label a urine specimen cup with labels that we provided
3. Read and record the total volume and time of urination on the Log, with the correct sample number from the label (e.g. S1-U01).
4. Pour ~ 60 mL of urine into the specimen cup and cap the cup. 5. Put the sample cup in the cooler
6. Discard the rest of urine, and seal the beaker in a 12 x 12 Zip-loc bag
7. If you prefer to use gloves for this procedure, please put the used gloves in the Zip-loc bag
8. We will collect the samples and wastes on Wednesday, Thursday, and Monday morning
Air Sampling Instruction
During the 8-day sample collection period
• Please collect personal air samples continuously for the 9 days.
• During weekdays, please change air samplers 4 time a day, at the following intervals
o In the morning before leaving home for work (e.g. 7 am) o In the morning after arriving work (e.g. 8 am)
o In the afternoon before leaving work (e.g. 5 pm) o In the afternoon after arriving home (e.g. 6 pm) • During the weekends
o Avoid driving
o If you will be driving (minimum 30 min), changing the air samplers before and after driving
o If you will be driving for less than 30 minutes, do not change the air sampler
o Otherwise, change air samplers at the same time points as the weekday
1. Read the time of air sampling from the pump timer for the existing tube (e.g. S1- A01) and record it on the Log.
2. Label a new air sample tube with labels that we provided (e.g. S1-A02), take off caps from both ends.
3. Turn off the pump (this will reset the timer), disconnect the existing tube (e.g. S1- A01) from the pump, cap the tube from both ends, and place it in a cooler. 4. Connect the new tube (e.g. S1-A02) to the pump, turn on the pump, and the time
will start from zero minute.
5. Record time of air sampler change with the corresponding air sample number. 6. We will collect the samples and wastes on Wednesday, Thursday, and Monday
morning.
Instructions on Dietary Intake
Recommended food for sampling days (Tue, Wed, Sat, Sun). • Beverages: Yes Water, juice, milk, and soda (limited)
No Coffee
• Snacks: Yes Assorted nuts, most diary products, non-chocolate candies, assorted non-chocolate cookies, jelly, egg
No Yogurt (flavored/frozen), chocolates, pies, cottage cheese
• Fruits and Vegetables:
Yes Cantaloupe, Cole slaw, strawberry, grapefruit, beans and
peas
No most greens
• Breakfast: Yes Blueberry muffin, corn muffin, whole oat cereal, corn flakes, bran cereal, bread (not toasted)
• Lunch (See below)
• Dinner: Yes Pre-packaged (preferred) or home-cooked roasted or strewed meat, steamed rice and vegetable
No Grilled, barbecued, smoked, and deep fried food
Table A.2 Lunch recommendation during study period.
Low PAH-containing diet (week 1) High PAH-containing diet (week 2) Meals
Type Portion Type Portion Pan fried burger from
McDonald’s or equivalents 112 g
Grilled burger from Burger
King or equivalents 176 g
Lunch
Potato chip 42 g Fries 102 g
During non-sampling days (Mon, Thu, Fri)
Avoid BBQ, grilled, smoked food, and fast food hamburgers. If you eat those foods, please note the type and amount of the food, and time of intake on the questionnaire.