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USP7 INHIBITON AS A MODEL FOR DNA REPLICATION TERMINATION

Groundwater and surface water interactions in the Upper Boulder River are more

complex than initially understood. It is apparent is that groundwater, rain, and snow are all major contributors to total streamflow generation in the Boulder River throughout the year. Our

investigation is limited because of the likely absence of an important endmember influencing the isotopic composition of the water. Soil water is likely a major endmember that is missing in this investigation. Based on the amounts of rain and snow that mix in the catchment, the large rain and snow contributions should be expected. The high groundwater fraction to flow suggests the river basin has high storage capacity, meaning that there is much groundwater present in

fractures in the subsurface.

This research has allowed the development of a baseline understanding of groundwater and surface water over multiple water years and placed a quantity on the relative proportions of source waters. In addition to establishing a baseline understanding, we were able to make repeatable observations and add to the knowledge base for the Upper Boulder River catchment.

Logical next steps for studying this area are to install soil lysimeters and sample and characterize the chemical fingerprint of soil water. In addition, searching for temporary seeps and springs discharging near the river could also provide adequate chemistry of lateral subsurface flow. This could then be used in a secondary mixing analysis to characterize their contributions to total flow. Groundwater age dating may also provide insight as to the residence time of groundwater mixing with the river system. Older groundwater would suggest deeper groundwater, while very young water ages may reflect shallow subsurface flows dominating. If deep groundwater was suspected of mixing with the catchment, then other isotopic

measurements more reflective of the geochemistry of deep groundwater (based on local geology) could be used.

Works Cited

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Carlson L. (2013). Managed Recharge and Base-Flow Enhancement in an Unconsolidated Aquifer in the Boulder River Valley, Montana. unpublished M.S. Thesis, Montana Tech of the University of Montana, 184 pp.

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Appendix A: Field Parameters and Isotopes

Table IV: Bernice field parameters and isotopes (δD and δ18O) Bernice Time SC Temp °C Alkalinity

1/22/2012 River Frozen 2/5/2012 River Frozen 2/20/2012 River Frozen 3/3/2012 River Frozen

4/1/2012 18:00 112 4.7 -142 -17.9

11/22/2014 River Frozen 1/20/2014 River Frozen 3/8/2014 River Frozen

5/3/2014 13:40 88 6.3 43 -141 -17.8

6/21/2014 15:00 77 16.1 19 -138 -17.6

9/2/2014 14:50 133.6 16.4 64 -137 -17.2

11/21/2014 River Frozen

Table V: High Ore field parameters and isotopes (δD and δ18O)

Table VI: USGS field parameters and isotopes (δD and δ18O)

Appendix B: Anions

Table VII: Bernice anions

Bernice Analyte F Cl PO4-P SO4

Table VIII: High Ore anions

High Ore Analyte F Cl PO4-P SO4

Table IX: USGS Gauging Station anions

USGS Analyte F Cl PO4-P SO4

(mg/L) (mg/L) (mg/L) (mg/L)

Detect Limit 0.0500 0.3 0.5 0.6

Date Time

5/21/2011 14:30 <0.05 0.82 <0.5 8.6

6/4/2011 10:15 <0.05 0.73 <0.5 7.7

6/18/2011 13:25 <0.05 0.62 <0.5 7.2

7/2/2011 12:20 <0.05 0.49 <0.5 5.7

7/29/2011 12:32 0.054 0.83 <0.5 9.8

8/14/2011 13:40 0.058 0.97 <0.5 11.0

9/9/2011 15:13 0.066 1.1 <0.5 12.3

9/24/2011 11:30 0.093 2.8 0 10.8

10/10/2011 10:16 0.108 2.9 0 10.5

10/24/2011 13:50 0.090 2.8 0 11.0

11/5/2011 11:00 0.086 2.7 0 11.7

12/4/2011 14:15 0.093 2.8 0 12.3

12/17/2011 13:00 0.088 2.8 0 12.4

1/22/2012 15:00 0.088 2.8 0 12.4

Appendix C: Cations

Table X: Bernice cations

Bernice Ca K Mg Na Si

1/22/2012 River Frozen 2/5/2012 River Frozen 2/20/2012 River Frozen 3/3/2012 River Frozen

4/1/2012 18:00 12.77 1.63 2.51 6.11 7.13

Table XI: High Ore Cations

Table XII: USGS cations

Appendix D: Alkalinity

Figure 27: Alkalinity in the Upper Boulder River 0

10 20 30 40 50 60 70 80

mg/L as CaCO3

Alkalinity in Surface Water Samples Alk (USGS)

Alk (High Ore) Alk (Bernice)

Appendix E: Na-Cl ratios

Plotting the record available for sodium (Na) against chloride (Cl) we observe that there is not a one to one ratio between sodium and chloride at Bernice. The ratio becomes closer to one to one downstream of Bernice. It is possible that chloride is entering the river between Bernice and High Ore from either road salts or the salt shack downstream of Bernice. However, even in baseflow there is no net addition of sodium to the river, indicating that the chloride added to the river is from a source other than road salts.

Figure 28: Ratio Na-Cl for three surface water sites over the water year 0

0.1 0.2 0.3 0.4

0 0.1 0.2 0.3 0.4

Cl (meq)

Na (meq)

Cl-Na Ratios (meq)

Cl-Na (USGS) Cl-Na (High Ore) Cl-Na (Bernice) Linear (Unity)

Appendix F: Groundwater Chemistry Values

Table XIII: Well 53488 groundwater chemistry 53488 Well Anions/Cations, Alkalinity, Stable Isotopes

Major Ion Results

Total Cations 4.082 Total Anions 4.249

Alkalinity as CaCO3 (mg/L): 123.85

Field pH: 6.79

Field Conductivity (μS/cm) 413

δ18O (‰) -18.3

δD (‰) -140

Table XIV: Well 163982 groundwater chemistry 163982 Well Anions/Cations, Alkalinity, Stable Isotopes

Major Ion Results

Total Cations 3.404 Total Anions 3.534

Alkalinity as CaCO3 (mg/L): 119.8

Field pH: 6.79

Field Conductivity (μS/cm) 336

δ18O (‰) -16.8

δD (‰) -145

Table XV: Well 265184 groundwater chemistry 265184 Well Anions/Cations, Alkalinity, Stable Isotopes

Major Ion Results

Total Cations 2.66 Total Anions 2.663

Alkalinity as CaCO3 (mg/L): 87.76

Field pH: 6.94

Field Conductivity (μS/cm) 267

δ18O (‰) -18.2

δD (‰) -139

Table XVI: Well 266999 266999 Well Anions/Cations, Alkalinity, Stable Isotopes

Major Ion Results

Total Cations 3.794 Total Anions 3.923

Alkalinity as CaCO3 (mg/L): 162.39

Field pH: 7.55

Field Conductivity (μS/cm) 370

δ18O (‰) -19.3

δD (‰) -148

Appendix G: SNOTEL Dataset Breakdown

Figure 29: Snotel Dataset for Frohner Meadow over the water years where sampling took place.

Accumulated snowmelt is sometimes in excess of accumulated precipitation for the dataset.

Figure 30: Snotel Dataset for Tizer Basin over the water years where sampling took place. Accumulated snowmelt is sometimes in excess of accumulated precipitation for the dataset.

-600

Frohner Meadow Precipitation Accumulation and Type Month by Month

Monthly Accumulation Snow Monthly Accumulation Rain

Precipitation Accumulation (mm) Snow Water Equivalent (mm)

Median Precipitation Accumulation (1981-2010) (mm) Median Snow Water Equivalent (1981-2010) (mm)

-600

Tizer Basin Precipitation Accumulation and Type Month by Month

Snow Rain

Precipitation Accumulation (mm) Snow Water Equivalent (mm)

Median Precipitation Accumulation (1981-2010) (mm) Median Snow Water Equivalent (1981-2010) (mm)

Appendix H: Precipitation Chemistry

Table XVII: NADP Site MT07 precipitation information 2011-2013

Site ID MT07

Summary Period 2011 2012 2013

Ca (mg/L) 0.085 0.272 0.154

Mg (mg/L) 0.011 0.029 0.017

K (mg/L) 0.016 0.029 0.020

Na (mg/L) 0.025 0.059 0.023

NH4 (mg/L) 0.182 0.172 0.285

NO3 (mg/L) 0.401 0.501 0.553

Cl (mg/L) 0.037 0.096 0.050

SO4 (mg/L) 0.249 0.255 0.320

Lab pH 5.430 5.450 5.670

Lab Cond. uS/cm 3.960 5.190 4.630

Cation/Anion Ratio 1.610 2.010 1.680

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