°C degree Celsius
µ micro
AGM aorta-gonads-mesonephros
AML acute myeloid leukaemia
amp ampicillin
APC allophycocyanine
approx approximately
BC blood cell
bFGF bovine fibroblast growth factor BMP4 bone morphogenic protein 4
bp base pair
BSA bovine serum albumin
CAG chicken beta actin
cat no catalogue number
Clnd5 claudin5
CMV cytomegalovirus
d day
D diffusion coefficient
DiI 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate DiI-Ac-LDL 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate
acetylated low density lipoprotein DNA deoxyribonucleic acid
DMSO dimethyl-sulfoxide
dpc days post coitum
E embryonic day
EC endothelial cell
E-Cadherin epithelial Cadherin
EDTA ethylene-diamin-tetraacetic acid
e.g. example given
Ep embryo proper
ES embryonic stem
ESC embryonic stem cell
F Farad
FACS fluorescence activated cell sorting
FCS foetal calf serum
Fig figure
FITC fluoresceinisothiocyanate Flk1 foetal liver kinase 1
Flt fms-related tyrosine kinase
FLP FLP recombinase FLPe enhanced FLP recombinase FSC forward scatter g gram G418 geniticin GB giga byte
G-CSF granulocyte colony stimulating factor
h hour
H hight
H2B histone 2B
HBSS Hank's buffered saline solution HSC hematopoietic stem cell
hsp heat shock protein
kDa kilo Dalton
IFN interferon
IL interleukin
IPTG isopropyl-β-D-thiogalactopyranoside
IRES internal ribosomal entry site
L litre
LB LB broth base
LIF leukaemia inhibitory factor
LTR-HSC long-term repopulating hematopoietic stem cells
M molar
m milli
M-CSF macrophage colony stimulating factor MEM minimal essential medium
min minutes
neo neomycin (geniticin) nucmem nuclear membrane
OHT 4-hydroxy-tamoxifen
PAGE polyacrylamide gelelectrophoresis PBS phosphate buffered saline
PCR polymerase chain reaction
PE phycoerythrine
PI propidium iodide
P-Sp para-aortic splanchnopleura
puro puromycin
RCF radial central force
rpm rounds per minute
RT room temperature
Sca-1 stem cell antigen 1
SCF stem cell factor
SCL stem cell leukaemia
SDS sodium dodecyl sulfate
sec seconds
SMA smooth muscle actin
SSC side scatter
TAE Tris acetate EDTA
tal1 T cell acute leukaemia TBD tamoxifen binding domain
TTT Timm's Tracking Tool
V Volt
VE-Cad vascular endothelial cadherin VE-Cadherin vascular endothelial cadherin VEGF vascular endothelial growth factor
VEGFR vascular endothelial growth factor receptor
wt wildtype
Hiermit erkläre ich, dass ich die vorliegende Dissertation selbstständig und ohne unerlaubte Hilfe angefertigt habe. Ferner habe ich weder versucht, eine Dissertation einzureichen oder eine Doktorprüfung durchzuführen, noch wurde die Dissertation oder Teile derselben einer anderen Prüfungskommission vorgelegt.
München, der 07. August 2008
First, I would like to thank Timm Schroeder for being an excellent teacher and a patient supervisor. Thank you for motivating me, teaching me not only technical skills but also a lot more, and thank you for opening the way to Japan for me. I learned a lot from you – thank you very much!
I am very grateful to Shin-Ichi and Satomi Nishikawa their support and advice but even more for their kind invitation to work in their lab. I had two great stays in Japan which was significantly determined by the welcoming, warm and helpful atmosphere in your lab. Satomi, thank you very much for your excellent technical help and your patience in teaching me.
Thanks to Magdalena Götz for officially supervising my thesis and giving her input in seminars and in my thesis committee.
I am indebted to my lab-members for the excellent and helpful working atmosphere and for critical discussions. I learned a lot from you and your inputs significantly contributed to this study.
Michael, thank you for your support with time lapse imaging. Thank you, Angi and Christian, for helping me at unusual working hours and for your immediate support when it was needed urgently. Bernhard, thanks for fulfilling all my TTT-wishes and programming them as fast as possible. Special thanks to Andrea and Erin for critical reading of parts of this manuscript. Annemieke, I am very thankful that you had a critical look on my application forms. Andrea, I especially would like to thank you not only for your help with FACS but most importantly for your critical opinion, for all the meal and tee breaks and the intense discussions about life.
I would also like to thank Andrea Wizenmann und Leanne Godinho for the excursions into chicken and zebrafish biology.
Many people in my private environment contributed to this thesis by motivating me and being patient and understanding. Special thanks for this to my mother and brother, Susi, Anne, Bine and Verena.
Name Hanna Maria Eilken
Date of birth 07 August 1978
Place of birth Munich
Nationality german
Family status unmarried
Education:
09/1985 – 07/1998 High school Diploma (Abitur) 09/1998 – 05/2004 Master of Science Student
at the Ludwig-Maximilians-Universität München(Diploma, Biology)
Main subjects: Microbiology Cell biology Immunology Biochemistry 05/2004 – 10/2004 Short term research visit
in the Laboratory of Stem Cell Biology, RIKEN Center for Developmental Biology, Kobe, Japan
since 10/2004: PhD Thesis
at the Institute of Stem Cell Research at the Helmholtz Zentrum München. Supervisor is Prof. Dr. Magdalena Götz, Faculty of Biology of the Ludwig-Maximilians-Universität München