1 SOBRE DICOM Y PACS
1.4 WADO: UNA OPCION PARA EL ACCESO VIA WEB
Gln-Glu Leu-Leu Asp-Cys Asp-Arg- - - - -Arg-Ser-Tyr
Gly-Gly-Tyr Pro-Trp-Ser-Ala-Leu Gln
Gln-Gln Val-Asp Asp-cys Ala-Lys-
Gly-Cys Lys
80 90
�
1 00Phe-
Cys Asp-Val-
-
!leCys Arg-Ser-
1 1 0§
Ala-Leu-Gln-Asp-Gln-Lys-Tyr-Val-Thr-Ile Asp Thr-Tyr-Glu-Asn-Val-Pro-Tyr-Asn
Ar&-Glu-Lys-Gly-Pro-Tyr-Ala-Ala-Lys-Thr Asp Gly-Val-Arg-Gln-Val-Gln-Pro-Tyr Asn Gln-Gly-Ala-Leu-Leu
Lys-Ala-Arg-Val-Pro-Arg Asn Asn-Glu-S.er-Ser-Met-
A p B F A p B F A p B F A p B F 130 1 40 1 50 - �hr-Ala-Val-Thr-Tyr ln-Pro
Tyr-Ser-Ile-Ala-Aan Gln-Pro Val-Ser-Val-Vel-Leu-Gln-Ala-Ala -Gly-Lys-Asp-Phe -Gln-Leu Tyr Arg-Gly Gly Tyr-Ala-Val-Ser-Lys Gln-Pro Ile-His-Ala-Val-Thr-Ala-Ile -Gly-Tyr
1 60 1 7 0
-Ala -Val Asp-His -Ale Ile-Val -Ile V� l-Gly-Tyr Gly-Thr-Glu-Gly-Gly-Vel-
Val Gly-Pro-cys-Gly Asp-His-Ala Val -Ala -Ala v� l-Gly-Tyr Asn- - - -Pro-
1 80
�
1 90
Lys-Aan-Ser-Trp Asp Thr Thr ly-Tyr Met Arg Ile -Leu
Gly Tyr Gly Thr Gly Trp-Gly-Glu Asn Gly-Tyr Ile Arg Ile-Lys
Arg Trp-Gly-Glu Ala Gly-Tyr Ile Arg
Gly-Gly-Ala- -
Gly-Asn-Ser-Tyr Val S er-S er-Ser-S er Ile
2 1 0 220
ys-Gly Leu -Tyr-Thr-s�-Ser-Phe Tyr-Pro Val -Lys-Asn-COOH ys-Gly Ile-Ala-Ile-Asp-Pro-Leu Tyr-Pro Thr-Glu-Gly
Fig. 4 . 4. Homologies among the available sequence s of some plant
tbiol prot eolytic enzymes.
2 00
Asn-V� l-
Gly -Thr-
Asp-Val-
Spaces in the sequenc es ind icate possible delet ions or insert ions of amino ac ids in the respec t ive protein sequences .
S equence numbering as for t he s equenc e of act inidin.
4 \H \N
The det ermination of t he primary amino acid s equenc e of ac t i nidin fro• the trypt ic pept ide s has provided the first c omplete amino ac id primary sequenc e of a plant t hiol prot ease t or c oaparison with the sequenc e o f papain. The d egre e ot ho� ol ogy among t he sequenc e s ot papain and actinidin , and the fragment s of s equenc e available for other plant thiol proteases, d emonstrates t hat the s tructural c haract eristics of ac t inidin are v ery similar to those d et ermined so far for other plant t hiol prot eases , thus providing further evidence for these enzymes having evolved from a commo n ancestral prot ein.
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