View of Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO

Journal of Applied Research and Technology

www.jart.ccadet.unam.mx

Journal of Applied Research and Technology 16 (2018) 498-510

J. C. Méndez ^a, U. Arellano ^a, S. Solís ^a, M. Asomoza ^a,*, V. H. Lara ^a, A. J. Padilla ^a, J.A. Wang ^b

Received dd mm aaaa; accepted dd mm aaaa Available online dd mm aaaa

𝐶𝑖 𝐶𝑓𝑊

𝐶𝑖 𝐶𝑓𝑊

θ θ

θ

SBA-15

110 100

200

0 2 4 6 8 10

Intensity (A.U)

2-Theta (Degrees)

0 2 4 6 8 10

Intensity (A.U)

2-Theta (Degrees)

1Ca-SBA-15

0.5Ca-SBA-15

3Ca-SBA-15

θ

SBA-15 1Ca-SBA-15

0.5Ca-SBA-15 3Ca-SBA-15 111

220 100

SBA-15 0.5Ca-SBA-15 1Ca-SBA-15 3Ca-SBA-15

0.0 0.2 0.4 0.6 0.8 1.0

0 200 400 600 800 1000

Vol. adsorbed (cm3 g-1 STP)

Relative pressure P/P₀ SBA-15

1Ca-SBA-15 3Ca-SBA-15 0.5Ca-SBA-15

250nm

a) b)

c) d)

250nm

250nm 250nm

a) b) b)

c) d)

100nm 50 nm

100nm 100nm

Si Ka O Ka Ca Ka a)

Si Ka O Ka Ca Ka

b)

Si Ka O Ka Ca Ka

c)

10µm 10µm 10µm

0 1 2 3 4

C o u n ts ( a .u )

K eV

Ca Ca

O Si

0 1 2 3 4

C o u n ts (a .u )

K eV

Ca Ca

O Si

0 1 2 3 4

C o u n ts (a .u )

K eV

Ca

Ca O Si

a)

b)

c) O

O

O

10µm

10µm

10µm

Element Weight %

O 60.23

Si 38.69

Ca 1.08

Element Weight %

O 57.51

Si 39.86

Ca 2.93

Element Weight %

O 57.81

Si 41.76

Ca 0.44

0 20 40 60 80 100 120

0 20 40 60 80 100

Adsorption of Rugosa Candida Lipasa (%)

Time (min)

SBA-15 0.5Ca-SBA-15 1Ca-SBA-15 3Ca-SBA-15

0 20 40 60 80 100 120

0 10 20 30 40 50

60 3Ca-SBA-15 1Ca-SBA-15 0.5Ca-SBA-15 SBA-15

ln(Co/C)

Time (min)

0 20 40 60 80 100 120

0 20 40 60 80 100

Adsorption of Rugosa Candida Lipasa (%)

Time (min)

SBA-15

0.5Ca-SBA-15

1Ca-SBA-15

3Ca-SBA-15

0 20 40 60 80 100 120 0

10 20 30 40 50 60

SBA-15 0.5Ca-SBA-15 1Ca-SBA-15 3Ca-SBA-15

ln(Co/C)

Time (min)

()

CKa CKa CKa CKa

NKa NKa NKa NKa

SiKa SiKa SiKa SiKa

RCL RCL



3Ca-SBA-15 Adsorption pH 3.5

- - -- -

-- -- -- -

-

- -- -- -

- -

- - -

-

- -

- - -

-

- - -

- +

+ +

+

+ + +

CaO

‐