[PDF] Top 20 EL DESARROLLO DE LA CREATIVIDAD MEDIANTE LA UTILIZACIÓN DE MATERIAL RECICLABLE EN NIÑOS Y NIÑAS DE 3 AÑOS DE EDAD GUÍA DE APLICACIÓN CON MATERIAL RECICLABLE DESTINADO A PADRES DE FAMILIA DEL CENTRO DE DESARROLLO INFANTIL "LAS GAVILLAS" UBICADO EN EL DISTRITO METROPOLITANO DE QUITO EN EL AÑO 2013 2014
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Genetic analysis of the nsP3 region of Sindbis virus: evidence for roles in minus-strand and subgenomic RNA synthesis.
... These data showed that increased replication of the CR3.36 and CR3.39 genomes occurred during complementation and indicate that the ts defect is most likely a defect in nsP3 or nsP3-cont[r] ... See full document
129
Fate of Minus-Strand Templates and Replication Complexes Produced by a P23-Cleavage-Defective Mutant of Sindbis Virus
... SIN2V minus-strand synthesis was normal, as it was dependent on newly translated proteins, and the minus strands accumulated in an RI that must have been part of the RTC and functioned as ... See full document
169
Sindbis virus RNA-negative mutants that fail to convert from minus-strand to plus-strand synthesis: role of the nsP2 protein.
... and/or nsP3, since these and other A comple- mentation group mutants have so far failed to complement either nsP2 or nsP3 mutants (44, ...and nsP3 regions of each virus were cloned and ... See full document
Initiation of minus-strand RNA synthesis by the brome mosaicvirus RNA-dependent RNA polymerase: use of oligoribonucleotide primers.
... CTP). The amounts of ATP and UTP per reaction were kept at 2.0 mM, but concentrations of GTP and CTP were varied as appropriate for the particular experiment. After a 90-min incubation, reaction mixtures were usually ... See full document
Role of the 3′ tRNA-Like Structure in Tobacco Mosaic Virus Minus-Strand RNA Synthesis by the Viral RNA-Dependent RNA Polymerase In Vitro
... TMV RNA could help to distinguish these possibilities, as well as defining the structural requirements for RdRp interactions with satellite TMV RNA, which also has a 3⬘-TLS and upstream pseudoknotted domain ... See full document
9
Subgenomic Negative-Strand RNA Function during Mouse Hepatitis Virus Infection
... The RNA was extracted with an equal volume of low-pH (pH ...The RNA was collected by centrifugation, dried, and resuspended in sterile, deionized, diethylpyrocarbonate-treated water for mRNA and RF ... See full document
4
Secondary Structure of the 3′ Terminus of Hepatitis C Virus Minus-Strand RNA
... minimal RNA frag- ment required for efficient replication of the (⫺) strand spans nt ⫺239 to ⫺1 (43), which corresponds to the domain I iden- tified in the present ...of RNA synthesis ...IRES ... See full document
5
Alphavirus Minus-Strand RNA Synthesis: Identification of a Role for Arg183 of the nsP4 Polymerase
... the virus was ...efficient minus-strand replicase activity by the nsP4-containing ...of minus-strand synthesis that it gave compared to Lys183 nsP4 ...made analysis of ... See full document
8
Replicase-Binding Sites on Plus- and Minus-Strand Brome Mosaic Virus RNAs and Their Roles in RNA Replication in Plant Cells
... mosaic virus (BMV) RNA synthesis have been characterized primarily for ...and minus-sense fragments, were constructed and tested for binding enriched BMV replicase in a template competition ... See full document
3
Minimal Template Requirements for Initiation of Minus-Strand Synthesis In Vitro by the RNA-Dependent RNA Polymerase of Turnip Yellow Mosaic Virus
... structured RNA in more detail, fragments which are not related to the 39 terminus of TYMV RNA were tested as ...TYMV RNA upstream of the tRNA-like structure ...double-stranded RNA product in a ... See full document
Incomplete removal of the RNA primer for minus-strand DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase.
... For the experiments in which the fate of the RNA primer was determined, the T348 fragment was excised from the single-stranded vector with EcoRI as previously described 5; the resulting [r] ... See full document
11
A Transcriptionally Active Subgenomic Promoter Supports Homologous Crossovers in a Plus-Strand RNA Virus
... sg RNA concentration may depend upon such postevent factors as RNA stability, encapsidation, RNA transport, posi- tional effects favoring synthesis of shorter sg RNAs, or other, yet unknown, ... See full document
16
In Vitro Synthesis of Minus-Strand RNA by an Isolated Cereal Yellow Dwarf Virus RNA-Dependent RNA Polymerase Requires VPg and a Stem-Loop Structure at the 3′ End of the Virus RNA
... plus-strand RNA genomes lack a 7-meth- ylguanosine cap structure but instead have a covalently linked virus-encoded protein (VPg) at the 5 ⬘ ...plus-strand RNA viruses, which are ... See full document
5
Maintaining the structural integrity of the bamboo mosaic virus3′ untranslated region is necessary for retaining the catalytic constant for minus strand RNA synthesis
... BaMV RNA for minus-strand RNA synthesis was identified in an in vitro replication assay by using the replicase com- plex that was derived from BaMV-infected Nicotiana benthamiana ... See full document
101
Deletion analysis of the capsid protein of Sindbis virus: identification of the RNA binding region.
... Our studies identified a 32-amino-acid region that is essential for the specificity in RNA binding, and they defined a 68-amino-acid minimal sequence which displays almost the complete s[r] ... See full document
118
In Vitro Analysis of Human Immunodeficiency Virus Type 1 Minus-Strand Strong-Stop DNA Synthesis and Genomic RNA Processing
... for RNA synthesis. RNA was purified by electrophoresis on a 5% denaturing polyacrylamide gel, excised, eluted by soaking overnight in 10 mM Tris Cl (pH ...8.0). RNA was quantitated using a UV ... See full document
50
Cellular Proteins from Human Monocytes Bind to Dengue 4 Virus Minus-Strand 3′ Untranslated Region RNA
... of RNA polymerase III ...5⬘ region of our 3⬘UTR RNA contains a U-rich track, we performed a deletion of 6 nt, from nt 101 to 96 (CCUUUU), later referred to as del-96 ...single-strand ... See full document
1
Specific detection of hepatitis C virus minus strand RNA in hematopoietic cells
... clinical evidence suggests that some of these genotypes may be more virulent, have higher circulating titers or can be more readily transmit- ted as reflected by differential sensitivity of various genotypes to ... See full document
180
Mutagenesis of the 3' nontranslated region of Sindbis virus RNA.
... These include 14 nucleotide substitutions in the 3' terminal 20 nucleotides; 17 deletion mutants in which from 1 to 8 nucleotides in the 3' terminal 25 nucleotides are deleted; 6 large d[r] ... See full document
1
Specific Sindbis virus-coded function for minus-strand RNA synthesis.
... In cultures infected with tsll of complementation group B, the synthesis of viral minusstrand RNA ceased, whereas the synthesis of 42S and 26S plus-strand RNAs continued for at least 5 h[r] ... See full document
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