[PDF] Top 20 Metodología de la investigación en Ciencias de La Salud
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Increased triacylglycerol production in oleaginous microalga Neochloris oleoabundans by overexpression of plastidial lysophosphatidic acid acyltransferase
... model microalga Chlamydomonas reinhardtii, two sets of homologous enzymes catalyze two distinct and parallel TAG biosyn- thesis pathways, one in the plastid (prokaryotic path- way) and the other in the endoplasmic ... See full document
5
Accelerated triacylglycerol production and altered fatty acid composition in oleaginous microalga Neochloris oleoabundans by overexpression of diacylglycerol acyltransferase 2
... Neochloris oleoabundans, like many microalgae, accu- mulates neutral lipids under N-starvation condition [22, 24, 27]. To evaluate the lipid-production potential of the transformants, neutral lipids ... See full document
10
Dual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in Phaeodactylum tricornutum
... microalgal triacylglycerol (TAG) pathway, we overexpressed two key lipogenic genes, glycerol‑3‑phosphate acyltransferase (GPAT1) and lysophosphatidic acid acyl‑ transferase (LPAT1), in ... See full document
104
Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
... the oleaginous microalga Neochloris oleoabundans, with a focus on the complex interaction of pathways associated with the production of the triacylglycerol (TAG) biofuel ... See full document
17
Lysophosphatidic acid signalling in development
... glycerophosphate acyltransferase (GPAT) can convert PA to LPA (Page s et ...the production of LPA is regulated by the ̀ availability of precursor metabolites as well as the expression of catalytic ... See full document
13
The pivotal role of malic enzyme in enhancing oil accumulation in green microalga Chlorella pyrenoidosa
... fatty acid accumulation and desaturation ...fatty acid composi- tion was altered in the engineered lines compared to wild type ...cells. Overexpression of ME from the oleaginous fungus ... See full document
206
Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Brassica napus L.) using CRISPR-Cas9 system
... 3-phosphate acyltransferase (GPAT), lysophosphatidic acid acyltransferase (LPAT) and diacylglycerol acyl- transferase (DGAT) are involved in TAG biosynthesis in that order ...that ... See full document
6
Elevated plasma levels of lysophosphatidic acid and aberrant expression of lysophosphatidic acid receptors in adenomyosis
... Given the important roles of the receptor-mediated LPA signaling in both reproductive tract function and gynecological cancers, it will be informative to investigate the potential role of LPA in the development of adeno- ... See full document
20
Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica
... scalable production of biofuel [18]; however, few microalgal strains found in nature are endowed with the wide array of traits demanded by a large-scale and eco- nomically competitive production scheme ... See full document
54
Molecular characterization of a glycerol-3-phosphate acyltransferase reveals key features essential for triacylglycerol production in Phaeodactylum tricornutum
... (PUFA) increased by 41 %. Notably, C16:3 fatty acid was 52 % higher than that of wild-type cells, and C20:5 was almost 40 % higher; how- ever, C16:0 was about 45 % ...fatty acid composition were ... See full document
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Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylum tricornutum
... fatty acid profiling of TAGs, TAGs were separated from total lipid classes using thin-layer chromatography (TLC) and the fatty acid profile was ...fatty acid data of both total lipids and TAGs, since ... See full document
13
Lysophosphatidic acid is a major serum noncytokine survival factor for murine macrophages which acts via the phosphatidylinositol 3 kinase signaling pathway
... Lysophosphatidic acid (LPA) is the smallest and structur- ally simplest of all the glycerophospholipids. It occurs nor- mally in serum and binds with high affinity to albumin, while retaining its biological ... See full document
6
Draft genome sequence and detailed characterization of biofuel production by oleaginous microalga Scenedesmus quadricauda LWG002611
... glycerol-3-phosphate acyltransferase (GPAT), which catalyze the formation of Lysophosphatidic acid (LPA) from ...diacylglycerol acyltransferase (DGAT), indicating another rate limiting step ... See full document
35
Increased production of ganoderic acids by overexpression of homologous farnesyl diphosphate synthase and kinetic modeling of ganoderic acid production in Ganoderma lucidum
... Generation of homologous FPS‑overexpressing G. lucidum The G. lucidum FPS gene was amplified by genomic PCR with the primers FPS-NheI-F and FPS-SmaI-R. To over- express the FPS gene, the plasmid pJW-EXP-FPS (Addi- tional ... See full document
5
Modifications of the metabolic pathways of lipid and triacylglycerol production in microalgae
... oleic acid by the catalysis of FAD2 (ω6) ...fatty acid desaturases should result in an increased percen- tage of oleic acid present, relative to total fatty acid con- ...various ... See full document
123
Effect of diacylglycerol acyltransferase 2 overexpression in 3T3-L1 is associated to an increase in mono-unsaturated fatty acid accumulation
... Although the function of DGAT2 has been described solely as the catalysis of TAG formation, we found that the expression of a great number of adipogenic genes was affected by DGAT2 overexpression. DAG, an ... See full document
11
Manipulation of the carbon storage regulator system for metabolite remodeling and biofuel production in Escherichia coli
... the production of target compounds from various codon- optimized heterologous ...amorphadiene production both employ acetyl-CoA as a branch-point precursor (Figure 4b and Figure 4c), we hypothesized that ... See full document
32
Effective cultivation of microalgae for biofuel production: a pilot-scale evaluation of a novel oleaginous microalga Graesiella sp. WBG-1
... Several studies have demonstrated the importance of photosynthesis efficiency on oil formation [18, 21, 23]. In other words, normal photosynthesis is needed to produce enough carbon flux and drive the biosynthesis of ... See full document
27
L-histidine inhibits production of lysophosphatidic acid by the tumor-associated cytokine, autotaxin
... Fig. 1 shows that L-histidine inhibited ATX-stimulated migration of both A2058 human melanoma cells and SKOV-3 human ovarian carcinoma cells in a concentra- tion-dependent manner. Addition of 10 mM L-histidine to ... See full document
18
Development of Improved Formulation for Neochloris oleoabundans Via an Automated Microalgal Nutrient Screening System
... growth rate of microalgae compared to flue gas [15]. Some microalgae species are able to grow with an organic compound such as sugars, molasses and acetic acid (heterotrophic or mixotrophic condition) [14]. Other ... See full document
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