[PDF] Top 20 VICTOR GARCIA TOMA - Teoría Del Estado y Derecho Constitucional
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hnRNP A1 interacts with the genomic and subgenomic RNA promoters of Sindbis virus and is required for the synthesis of G and SG RNA
... that hnRNP A1 binds to the G and SG promoters, the consequences of this interaction for viral RNA synthesis are not ...that hnRNP A1 re-localizes from ... See full document
246
hnRNP A1 Interacts with the 5′ Untranslated Regions of Enterovirus 71 and Sindbis Virus RNA and Is Required for Viral Replication
... prototype virus of the family Togaviridae, genus ...positive-strand RNA genome, which is approx- imately 11,700 nt in length, has a 5 ⬘ cap and is polyadenylated at its 3 ⬘ ...nome RNA is translated ... See full document
91
In Vitro Synthesis of Sindbis Virus Genomic and Subgenomic RNAs: Influence of nsP4 Mutations and Nucleoside Triphosphate Concentrations
... vitro synthesis of Sindbis virus ...viral RNA synthesis in a system making both SG and G ...system, SG RNA was always made in excess of G RNA, ... See full document
127
Infectious Bursal Disease Virus Capsid Protein VP3 Interacts both with VP1, the RNA-Dependent RNA Polymerase, and with Viral Double-Stranded RNA
... VP3- RNA interaction seemed most ...the RNA was indeed of IBDV origin and that it contained both positive and negative strands of each of the two genome ...minus RNA strand, the results indicated ... See full document
145
A Minigenome Study of Hazara Nairovirus Genomic Promoters
... negative-strand RNA viruses (sNSV) are divided into families with either two (Arenaviridae, ...Lassa virus), three (Bunyaviridae, e.g., CCHFV and La Crosse virus [LACV]), or six to eight ... See full document
125
Noncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes
... viral genomic RNA (vgRNA) by the host 5= ¡3= exoribonuclease XRN1/Pacman ...(DB) RNA structures within the flaviviral 3= untranslated region (UTR), re- sulting in the accumulation of sfRNA ... See full document
29
An RNA Pseudoknot Is Required for Production of Yellow Fever Virus Subgenomic RNA by the Host Nuclease XRN1
... the RNA molecules, which explains why they were not detected in the probing experiments and why enhancement of the contrast in the picture of the Northern blot was ...the RNA sequences and structures that ... See full document
150
Genetic analysis of the nsP3 region of Sindbis virus: evidence for roles in minus-strand and subgenomic RNA synthesis.
... These data showed that increased replication of the CR3.36 and CR3.39 genomes occurred during complementation and indicate that the ts defect is most likely a defect in nsP3 or nsP3-cont[r] ... See full document
129
Role of Sindbis Virus Capsid Protein Region II in Nucleocapsid Core Assembly and Encapsidation of Genomic RNA
... a virus that had medium-sized plaques, while the remaining deletions all resulted in viruses with small plaques, suggesting defects in virus ...medium-plaque virus C ⌬ (96–100) had reduced growth ... See full document
239
Synthesis of Genomic and Subgenomic RNA in Mosquito Cells Infected with Two Sindbis Virus nsP4 Mutants: Influence of Intracellular Nucleoside Triphosphate Concentrations
... extract RNA, Trizol reagent was pipetted onto cell monolayers (1 ml/60-mm plate), and the cells were lysed by repeated ...⫻ g for 15 min, the aqueous phase was removed, and the RNA was precipitated ... See full document
109
Identification and Functional Analysis of the Turnip Yellow Mosaic Tymovirus Subgenomic Promoter
... duplicated sg promoter ...the genomic RNA of mutants tLs, tMs, and tSs, with fragments of 494, 257, and 88 nt, respectively, containing the tymobox region inserted in the sense orientation into the ... See full document
1
Looking for inhibitors of the dengue virus NS5 RNA-dependent RNA-polymerase using a molecular docking approach
... dengue virus (DENV) nonstructural protein 5 (NS5) contains both an N-terminal methyltransferase domain and a C-terminal RNA-dependent RNA polymerase ...viral RNA synthesis by a de novo ... See full document
8
DNA-Directed Expression of Functional Flock House Virus RNA1 Derivatives in Saccharomyces cerevisiae, Heterologous Gene Expression, and Selective Effects on Subgenomic mRNA Synthesis
... a subgenomic RNA3 ...not required for any step of the FHV life cycle, and the presence of an initiation codon in the corresponding region of the pro- tein A ORF is not conserved in other nodaviruses ...not ... See full document
26
Moloney Murine Sarcoma Virus Genomic RNAs Dimerize via a Two-Step Process: a Concentration-Dependent Kissing-Loop Interaction Is Driven by Initial Contact between Consecutive Guanines
... FIG. 1. MuSV RNA sequences involved in dimerization. (A) Representation of the MuSV genome. Numbering is with respect to the viral mRNA, which begins at the transcriptional initiation site and ends at the ... See full document
6
SCE1, the SUMO-Conjugating Enzyme in Plants That Interacts with NIb, the RNA-Dependent RNA Polymerase of Turnip Mosaic Virus, Is Required for Viral Infection
... lactopyranoside; Invitrogen), and incubated at 28°C overnight and then at 25°C for 2 h. Proteins were separated by 7.5% SDS-PAGE and blotted onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad) by stan- dard ... See full document
61
Discontinuous Subgenomic RNA Synthesis in Arteriviruses Is Guided by an RNA Hairpin Structure Located in the Genomic Leader Region
... of subgenomic (sg) mRNAs, which are used to express structural proteins and sometimes accessory ...the genomic 5 ⴕ ...minus-strand RNA synthesis in which base pairing between sense and ... See full document
5
RNA Structures Required for Production of Subgenomic Flavivirus RNA
... vitro-transcribed RNA into BHK-21 cells. At 72 h after electroporation, RNA was isolated and sfRNA production was investigated by Northern blotting using a 3 ⬘ -UTR-specific ...an RNA (sfRNA2) ... See full document
261
Reduced synthesis of Sindbis virus negative strand RNA in cultures treated with host transcription inhibitors.
... Sensitivity of viral RNA synthesis to a-A' TCA-precipitable cpm x103 Treatment None a-A 49 SYNTHESIS OF SINDBIS VIRUS NEGATIVE STRAND RNA fraction of control in: CHO cells CHOama-1 72.0 [r] ... See full document
93
Norovirus Genome Circularization and Efficient Replication Are Facilitated by Binding of PCBP2 and hnRNP A1
... require RNA-RNA contacts in the CS but the participation of cellular proteins is required to stabilize these ...5=-3= RNA- RNA interactions, we selected proteins for further study on ... See full document
196
Asynchronous Accumulation of Lettuce Infectious Yellows Virus RNAs 1 and 2 and Identification of an RNA 1 trans Enhancer of RNA 2 Accumulation
... in genomic RNA replication. The Beet yellows virus (BYV; genus Closterovirus) HSP70 homolog is likely involved in inter- and intracellular trafficking (3, 15, 18), and the BYV and LIYV HSP70 homologs ... See full document
16
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