Disciplina y otras variables

A published known single dominant gene designated Cre1 for resistance to a closely related species H. avenae (Ogbonnaya et al. 2001) was assessed in Turkey for its resistance in wheat, and was found to be effective. As a result the IWWIP (International Winter Wheat Improvement Program, Turkey, CIMMYT and ICARDA) breeding program has incorporated this source of resistance from the Australian spring bread wheat cultivar Silverstar into highly adapted winter wheat backgrounds. The Cre1 marker was optimised using the technique and optimised using STS-PCR method (Akar et al. 2009). This method was applied in a number of segregating winter wheat populations from F2-F4 containing Silverstar. Preliminary results indicate marker-assisted selection is functioning effectively with a recovery of Cre1 positive lines up to 88% depending on the cross. These marker positive lines are now being integrated into the breeding program. This tool has the potential to be used effectively on a larger scale to incorporate CCN resistance into wheat as has been achieved routinely in other breeding programs such as Australia. Furthermore, a number of Turkish germplasm have been checked for the presence of Cre1 gene, however to date none of germplasm have tested positive (Akar et al. 2009).

ACKNOWLEDGMENTS

The Turkish Ministry of Agriculture and ICARDA-CIMMYT Wheat Improvement Program is acknowledged for their financial and leadership support. Grains Research and Development Corporation (Australia), USAID (USA) and the Scientific and Technical Research Council (Turkey) are thanked for financial support. The collaboration of many national agricultural research programs (China, India, Iran and Tunisia) and several research institutions in Australia, USA, France, UK and Belgium are also gratefully acknowledged.

REFERENCES

Abidou H, El-Ahmed A, Nicol JM, Bolat N, Rivoal R, Yahyaoui A (2005) Occurrence and distribution of species of the Heterodera avenae group in Syria and Turkey. Nematologia Mediterránea 33, 197-203.

Akar T, Çalışkan M, Nicol JM, Uranbey S, Şahin E, Yazar S, William M, Braun HJ (2009) Molecular characterization of cereal cyst nematode diagnostic markers Cre1 and Cre3 in some winter wheat germplasm and their potential use against

Heterodera filipjevi. Field Crops Research (in press). doi:10.1016/j.fcr.2009.08.001

Balakhnina VP (1989) Resistance Heterodera avenae of varieties of Triticum durum Desf. and Triticum aestivum L. to the oat cyst nematode, In ‘Gelmintologiya segonya: problemy perspectivy. Tezisy dokladov nauchnoi konferentsii, Moskova, 4-6 April 1989, Moscow, USSR.’ pp. 36-37.

Bishnoi SP, Bajaj H (2002) Response of resistant barley cultivars to the Indian populations of Heterodera avenae complex. Indian Journal of Nematology 32, 125-128.

Cook R, Noel GR (2002) Cyst nematodes: Globodera and Heterodera species. In ‘Plant resistance to parasitic nematodes.’ (Eds JL Starr, R Cook, J Bridge) pp. 71-106. (CABI Publishing: Wallingford, UK)

Elekçioğlu İH, Avcı M, Nicol JM, Meyveci K, Bolat N, Yorgancılar A, Şahin E, Kaplan A (2004) The use of crop rotation as a means to control the cyst and lesion nematodes under rainfed wheat production systems. In ‘Proceedings of the first Turkish plant protection congress, 8-11 September 2004, Samsun, Turkey.’ pp. 85. [abstract]

Große E, Kohlmüller S (2004) Untersuchungen zur Verbreitung von Getreidezystennematoden nach einer neuen Differenzialmethode. Mitteilungen aus der Biologischen Bundesanstalt für Land- und Forstwirt- schaft Berlin- Dahlem 396, 563-564.

Holgado R, Andersson S, Rowe JA, Magnusson C, (2004) First record of Heterodera filipjevi in Norway, Nematologia Mediterránea 32, 205-211.

Nicol JM, Bolat N, Bagcı A et al. (2007) The international strategy for the incorporation of resistance in bread wheat against the soil borne pathogens (dryland root rot, cyst and lesion nematodes) using conventional and molecular tools. In ‘Wheat production in stressed environments. Proceedings of the seventh international wheat conference, 27 November-2December 2005, Mar del Plata, Argentina.’ (Eds HT Buck, JE Nisi, N Salomon) pp. 125-137. (Springer-Verlag GmbH: Heidelberg, Germany)

Nicol JM, Bolat N, Şahin E, Tülek A, Yıldırım AF, Yorgancılar A, Kaplan A, Braun HJ (2006) The cereal cyst nematode is causing economic damage on rainfed wheat production systems of Turkey. Phytopathology 96, S169. [abstract]

Nicol JM, Bolat N, Yıldırım AF, Yorgancılar A, Kılınç AT, Elekçioğlu İH, Şahin E, Erginbaş-Orakcı G, Braun HJ (2009) Identification of genetic resistance to cereal cyst nematode (Heterodera filipjevi) for international bread wheat improvement. In ‘Cereal cyst nematodes: status, research and outlook.’ (Eds IT Riley, JM Nicol, AA Dababat) pp. 160-165. (CIMMYT: Ankara, Turkey).

Nicol JM, Rivoal R (2008) Global knowledge and its application for the integrated control and management of nematodes on wheat. In ‘Integrated management and biocontrol of vegetable and grain crops nematodes.’ (Eds A Ciancio, KG Mukerji) pp. 243-287. (Springer Academic Publishing: The Netherlands)

Ogbonnaya FC, Subrahmanyam NC, Moullet O, de Majnik J, Eagles HA, Brown J. S, Eastwood RF, Kollmorgen J, Appels R and Lagudah ES (2001) Diagnostic DNA markersfor cereal cyst nematode resistance in bread wheat. Australian Journal of Agricultural Research 52, 1367-1374.

Rumpenhorst HJ, Elekçioğlu İH, Sturhan D, Öztürk G, Enneli S, (1996) The cereal cyst nematode Heterodera filipjevi (Madzhidov) in Turkey. Nematologia Mediterránea 24, 135-138.

Şahin E, Nicol JM, Elekçioğlu İH, Rivoal R (2009a) Hatching of Heterodera filipjevi in controlled and natural temperature conditions in Turkey. Nematology (in press).

Şahin E, Nicol JM, Elekçioğlu İH, Yorgancılar O, Yıldırım AF, Tülek A, Hekimhan H, Yorgancılar A, Kılınç AT, Bolat N, Erginbaş-Orakcı G (2009b) Frequency and diversity of cereal nematodes on the Central Anatolian Plateau of Turkey. In ‘Cereal cyst nematodes: status, research and outlook.’ (Eds IT Riley, JM Nicol, AA Dababat) pp. 100-105. (CIMMYT: Ankara, Turkey)

Şahin E, Nicol JM, Yorgancılar A, Elekçioğlu İH, Tülek A, Yıldırım AF, Bolat N (2008) Seasonal variation of field populations of Heterodera filipjevi, Pratylenchus thornei and P. neglectus on winter wheat in Turkey. Nematologia Mediterránea 36, 51-56.

Smiley RW, Yan GP, Handoo ZA (2008) First record of the cyst nematode Heterodera filipjevi on wheat in Oregon. Plant Disease 92, 1136.

Subbotin SA, Sturhan D, Rumpenhorst HJ, Moens M (2003) Molecular and morphological characterisation of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology 5, 515-538.

Tanha Maafi Z, Subbotin SA, Moens M (2003) Molecular identification of cyst- forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS - rDNA sequences. Nematology 5, 99-111.