UN ENSAYO DE EDUCACIÓN INDIVIDUALIZADA

The following standard and special practices, safety equipment, and facilities apply to agents assigned to BSL-2.

11.3.1 STANDARDMICROBIOLOGICALPRACTICES FORBSL-2

The standard practices for handling BSL-2 are the same as for BSL-1 standard microbiological practices.

FIGURE 11.1 Hand washing is always an appropriate procedure to control the spread of potential harmful organisms.

11.3.2 SPECIALPRACTICES FORBSL-2

Because of the risk involved in handling more hazardous biological organisms than BSL-1, special procedures are to be employed as follows:

. Access to the laboratory is limited or restricted by the principal supervisor when work with infectious agents is in progress. In general, persons at increased risk of contracting infection or for whom infection may be unusually hazardous are not allowed in the work area or animal rooms.

Persons who are immunocompromised or immunosuppressed may be at unusual risk of acquiring infections.

. The principal supervisor establishes policies and procedures whereby only persons who have been advised of the potential hazard and meet specific requirements (e.g., immunization) enter the work area or animal rooms.

. When an infectious agent requires special provisions (e.g., immunization) for entering a work area where it is in use, a hazard warning sign incorp-orating the universal biohazard symbol is posted on the access door to the laboratory work area. The hazard warning sign identifies the infectious agent, lists the name and telephone number of the principal supervisor or other responsible person, and indicates the special requirements for entering the area.

. Personnel are to receive appropriate immunizations for the agents handled or potentially present in the work area.

. When appropriate, baseline serum samples for work area and other at-risk personnel are collected and stored. Additional specimens may be collected periodically.

. A biosafety manual shall be developed for the specific work area. Personnel are advised of special hazards and are required to read and follow instruc-tions on practices and procedures.

. Personnel are to receive appropriate training on the potential hazards associated with the work involved, the necessary precautions to prevent exposures, and the exposure evaluation procedures. Personnel must also receive annual retraining and receive additional training when procedures or policies change.

. A high degree of precaution must always be taken with any contaminated sharp items, including needles and syringes, slides, pipettes, capillary tubes, and scalpels. Needles and syringes should be used in the work area only when there is no alternative, such as when parenteral injection, phlebotomy, or aspiration offluids from animals and diaphragm bottles are conducted.

Plastic ware should be substituted for glassware whenever possible.

. Only needle-locking syringes or disposable syringe-needle units (i.e., the needle is integral to the syringe) are used for injection or aspiration of infectious materials. Used disposable needles must not be bent, sheared, broken, recapped, removed from disposable syringes, or otherwise manipu-lated by hand before disposal; rather they must be carefully placed in conveniently located puncture-resistant containers used for sharps disposal.

Nondisposable sharps must be placed in a hard-walled container for trans-port to a processing area for decontamination, preferably by autoclaving.

. Syringes that resheathe the needle, needleless systems, and other safe devices should be used when appropriate.

. Broken glassware must not be handled directly by hand but must be removed by mechanical means such as a brush and dustpan, tongs, or forceps. Containers of contaminated needles, sharp equipment, and broken glass are decontaminated before disposal.

. Cultures, tissues, and specimens of bodyfluids are placed in a container that prevents leakage during collection, handling, processing, storage, transport, or shipping.

. Equipment and work surfaces should be decontaminated with an appropri-ate disinfectant on a routine basis as well as after work with infectious material is finished and, especially, after overt spills, splashes, or other contamination by infectious materials. Contaminated equipment must be decontaminated before it is sent for repair or maintenance or packaged for transport.

. Spills or accidents that result in overt exposures to infectious materials are immediately reported to the principal or responsible supervisor. Medical evaluation, surveillance, and treatment are provided as appropriate at no cost to employees, and written records are maintained.

. Animals not involved in the work performed are not permitted in the work area.

11.3.3 SAFETY EQUIPMENT FORBSL-2

Properly maintained biological safety cabinets, preferably Class II, or other appro-priate personal protective equipment (PPE) or physical containment devices are used in the following cases:

. Procedures with a potential for creating infectious aerosols or splashes are conducted. These may include centrifuging, grinding, blending, vigorous shaking or mixing, sonic disruption, opening containers of infectious materials in which internal pressure may differ from ambient pressure, inoculating animals intranasally, and harvesting infected tissues from ani-mals or eggs.

. High concentrations or large volumes of infectious agents are used. Such materials may be centrifuged in the open work area if sealed rotor heads or centrifuge safety cups are used and if these rotors or safety cups are opened only in a biological safety cabinet.

. Face protection (goggles, mask, face shield, or other splatter guards) is used for anticipated splashes or sprays of infectious or other hazardous materials to the face when the microorganisms must be manipulated outside the biological safety cabinet.

. Protective laboratory coats, gowns, smocks, or uniforms designated for lab use are worn in the work area. This protective clothing is removed and left

in the work area before personnel leave for other areas (e.g., cafeteria, library, or offices) in the facility. All protective clothing is either disposed of in the work area or sent to the laundry service (only after being decon-taminated). They are never taken home.

. Personnel are to wear gloves when handling infected animals and when hands may come in contact with infectious materials or contaminated surfaces or equipment. Wearing two pairs of gloves may be appropriate;

if a spill or splatter occurs, the hand will be protected after the contaminated glove is removed. Gloves are disposed of when contaminated, removed when work with infectious materials is completed, and not worn outside the work area. Disposable gloves are not washed or reused.

11.3.4 F^ACILITIES(S^ECONDARYB^ARRIERS)^FORBSL-2

Facilities for BSL-2 organisms must have special design and construction features such as the following:

. Facilities that house restricted agents must be provided with lockable doors.

. Each work area must contain a sink for handwashing.

. Work area is designed so that it can be easily cleaned. Rugs are not appropriate in areas where biological organisms are present.

. Bench tops are to be impervious to water and resistant to acids, alkalis, organic solvents, and moderate heat.

. Furniture is to be sturdy, and spaces between benches, cabinets, and equipment accessible for cleaning.

. If the work area has windows that open, they must befitted with fly screens.

. Eyewash facility is to be readily available.

. Biological safety cabinets shall be installed in such a manner that fluctu-ations of the room supply and exhaust air do not cause them to operate outside their parameters for containment. Biological safety cabinets shall be located away from doors, from windows that can be opened, from heavily traveled work areas, and from other potentially disruptive equipment so as to maintain the biological safety cabinets’ airflow parameters for con-tainment.

. Illumination shall be adequate for all activities, avoiding reflections and glare, which could impede vision.