Estudios in vivo

Pearson correlation coefficient; PSY: phytoene synthase; QC: quality control; qPCR: quantitative PCR; RNA-seq: RNA sequencing; STS: stilbene synthase; TPS: terpene synthase; UFGT: UDP- glucose:flavonoid 3-O-glucosyltransferase; UHPLC-MS/MS: ultra-high performance liquid chromatography-tandem mass spectrometer; VOCs: volatile organic compounds; Vvi: Vitis vinifera; ZDS: ζ-Carotene desaturase; ΨStem: stem water potential.

Acknowledgement

This study was funded by the Italian Ministry of Agricultural and Forestry Policies (VIGNETO), the International Ph.D. Programme in the Genomics and Molecular Physiology of Fruit Plants (GMPF) of the Fondazione Edmund Mach International Research School Trentino (FEM- FIRS>T), the COST Action FA1106 Quality Fruit, the University of British Columbia (10R18459), and Natural Sciences and Engineering Research Council of Canada (10R23082).

56

References

Alonso R, Berli FJ, Bottini R, Piccoli P (2015) Acclimation mechanisms elicited by sprayed abscisic acid, solar UV-B and water deficit in leaf tissues of field-grown grapevines. Plant Physiol Biochem 91: 56–60

Anders S, Pyl PT, Huber W (2015) HTSeq—a Python framework to work with high-throughput sequencing data. Bioinformatics 31: 166–169

Battilana J, Emanuelli F, Gambino G, Gribaudo I, Gasperi F, Boss PK, Grando MS (2011) Functional effect of grapevine 1-deoxy-D-xylulose 5-phosphate synthase substitution K284N on Muscat flavour formation. J Exp Bot 62: 5497–5508

Carbonell-Bejerano P, Diago MP, Martínez-Abaigar J, Martínez-Zapater JM, Tardáguila J, Núñez-Olivera E (2014) Solar ultraviolet radiation is necessary to enhance grapevine fruit ripening transcriptional and phenolic responses. BMC Plant Biol 14: 183

Castellarin SD, Matthews MA, Di Gaspero G, Gambetta GA (2007a) Water deficits accelerate ripening and induce changes in gene expression regulating flavonoid biosynthesis in grape berries. Planta 227: 101–112

Castellarin SD, Pfeiffer A, Sivilotti P, Degan M, Peterlunger E, Di Gaspero G (2007b) Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit. Plant Cell Environ 30: 1381–1399

Cela J, Chang C, Munné-Bosch S (2011) Accumulation of γ- rather than α-tocopherol alters ethylene signaling gene expression in the vte4 mutant of Arabidopsis thaliana. Plant Cell Physiol 52: 1389–1400

Chapman DM, Roby G, Ebeler SE, Guinard JX, Matthews MA (2005) Sensory attributes of Cabernet Sauvignon wines made from vines with different water status. Aust J Grape Wine Res 11: 339–347

Chaves MM, Zarrouk O, Francisco R, Costa JM, Santos T, Regalado AP, Rodrigues ML, Lopes CM (2010) Grapevine under deficit irrigation: hints from physiological and molecular data. Ann Bot 105: 661–676

Costantini L, Malacarne G, Lorenzi S, Troggio M, Mattivi F, Moser C, Grando MS (2015) New candidate genes for the fine regulation of the colour of grapes. J Exp Bot 66: 4427–4440

Degu A, Hochberg U, Sikron N, Venturini L, Buson G, Ghan R, Plaschkes I, Batushansky A, Chalifa-Caspi V, Mattivi F, et al (2014) Metabolite and transcript profiling of berry skin during fruit development elucidates differential regulation between Cabernet Sauvignon and Shiraz cultivars at branching points in the polyphenol pathway. BMC Plant Biol 14: 188

Degu A, Morcia C, Tumino G, Hochberg U, Toubiana D, Mattivi F, Schneider A, Bosca P, Cattivelli L, Terzi V, et al (2015) Metabolite profiling elucidates communalities and differences in the polyphenol biosynthetic pathways of red and white Muscat genotypes. Plant Physiol Biochem 86: 24–33

Del Fabbro C, Scalabrin S, Morgante M, Giorgi FM (2013) An extensive evaluation of read trimming effects on Illumina NGS data analysis. PLoS ONE 8: e85024

Deluc LG, Quilici DR, Decendit A, Grimplet J, Wheatley MD, Schlauch KA, Mérillon JM, Cushman JC, Cramer GR (2009) Water deficit alters differentially metabolic pathways affecting important flavor and quality traits in grape berries of Cabernet Sauvignon and Chardonnay. BMC Genomics 10: 212

Demmig B, Winter K, Krüger A, Czygan FC (1988) Zeaxanthin and the heat dissipation of excess light energy in Nerium oleander exposed to a combination of high light and water stress. Plant Physiol 87: 17–24

Falginella L, Di Gaspero G, Castellarin SD (2012) Expression of flavonoid genes in the red grape berry of “Alicante Bouschet” varies with the histological distribution of anthocyanins and their chemical composition. Planta 236: 1037–1051

Fedrizzi B, Carlin S, Franceschi P, Vrhovsek U, Wehrens R, Viola R, Mattivi F (2012) D-optimal design of an untargeted HS-SPME-GC-TOF metabolite profiling method. Analyst 137: 3725–3731

Ferrandino A, Lovisolo C (2014) Abiotic stress effects on grapevine (Vitis vinifera L.): Focus on abscisic acid-mediated consequences on secondary metabolism and berry quality. Environ Exp Bot 103: 138–147

Gil M, Pontin M, Berli F, Bottini R, Piccoli P (2012) Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation. Phytochemistry 77: 89–98

57

Gómez-Plaza E, Mestre-Ortuño L, Ruiz-García Y, Fernández-Fernández JI, López-Roca JM (2012) Effect of benzothiadiazole and methyl jasmonate on the volatile compound composition of Monastrell grapes and wines. Am J Enol Vitic ajev.2012.12011

Griesser M, Weingart G, Schoedl-Hummel K, Neumann N, Becker M, Varmuza K, Liebner F, Schuhmacher R, Forneck A (2015) Severe drought stress is affecting selected primary metabolites, polyphenols, and volatile metabolites in grapevine leaves (Vitis vinifera cv. Pinot noir). Plant Physiol Biochem 88: 17–26

Grimplet J, Hemert JV, Carbonell-Bejerano P, Díaz-Riquelme J, Dickerson J, Fennell A, Pezzotti M, Martínez- Zapater JM (2012) Comparative analysis of grapevine whole-genome gene predictions, functional annotation, categorization and integration of the predicted gene sequences. BMC Res Notes 5: 213

Hannah L, Roehrdanz PR, Ikegami M, Shepard AV, Shaw MR, Tabor G, Zhi L, Marquet PA, Hijmans RJ (2013) Climate change, wine, and conservation. Proc Natl Acad Sci 110: 6907–6912

Hartmann T (2007) From waste products to ecochemicals: Fifty years research of plant secondary metabolism. Phytochemistry 68: 2831–2846

Herrera JC, Bucchetti B, Sabbatini P, Comuzzo P, Zulini L, Vecchione A, Peterlunger E, Castellarin SD (2015) Effect of water deficit and severe shoot trimming on the composition of Vitis vinifera L. Merlot grapes and wines. Aust J Grape Wine Res 21: 254–265

Higo K, Ugawa Y, Iwamoto M, Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database. Nucleic Acids Res 27: 297–300

Hochberg U, Degu A, Toubiana D, Gendler T, Nikoloski Z, Rachmilevitch S, Fait A (2013) Metabolite profiling and network analysis reveal coordinated changes in grapevine water stress response. BMC Plant Biol 13: 184 Hochberg U, Degu A, Cramer GR, Rachmilevitch S, Fait A (2015) Cultivar specific metabolic changes in grapevines

berry skins in relation to deficit irrigation and hydraulic behavior. Plant Physiol Biochem 88: 42–52

Höll J, Vannozzi A, Czemmel S, D’Onofrio C, Walker AR, Rausch T, Lucchin M, Boss PK, Dry IB, Bogs J (2013) The R2R3-MYB transcription factors MYB14 and MYB15 regulate stilbene biosynthesis in Vitis vinifera. Plant Cell 25: 4135–4149

Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C, et al (2007) The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature 449: 463–467

Klee HJ, Giovannoni JJ (2011) Genetics and control of tomato fruit ripening and quality attributes. Annu Rev Genet 45: 41–59

Kliebenstein DJ (2004) Secondary metabolites and plant/environment interactions: A view through Arabidopsis thaliana tinged glasses. Plant Cell Environ 27: 675–684

Love MI, Huber W, Anders S (2014) Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15: 550

Lund ST, Bohlmann J (2006) The molecular basis for wine grape quality-a volatile subject. Science 311: 804–805 Ma C, Wang X (2012) Application of the GINI correlation coefficient to infer regulatory relationships in transcriptome

analysis. Plant Physiol 160: 192–203

Ma DM, Wang Z, Wang L, Alejos-Gonzales F, Sun MA, Xie DY (2015) A genome-wide scenario of terpene pathways in self-pollinated Artemisia annua. Mol Plant 8: 1580–1598

Ma S, Shah S, Bohnert HJ, Snyder M, Dinesh-Kumar SP (2013) Incorporating motif analysis into gene co-expression networks reveals novel modular expression pattern and new signaling pathways. PLoS Genet 9: e1003840 Maere S, Heymans K, Kuiper M (2005) BiNGO: A Cytoscape plugin to assess overrepresentation of gene ontology

categories in biological networks. Bioinformatics 21: 3448–3449

Malacarne G, Costantini L, Coller E, Battilana J, Velasco R, Vrhovsek U, Grando MS, Moser C (2015) Regulation of flavonol content and composition in (Syrah×Pinot Noir) mature grapes: integration of transcriptional profiling and metabolic quantitative trait locus analyses. J Exp Bot 66: 4441–4453

Martin DM, Aubourg S, Schouwey MB, Daviet L, Schalk M, Toub O, Lund ST, Bohlmann J (2010) Functional annotation, genome organization and phylogeny of the grapevine (Vitis vinifera) terpene synthase gene family based on genome assembly, flcdna cloning, and enzyme assays. BMC Plant Biol 10: 226

58

In document REGENERACIÓN ÓSEA VERTICAL A BASE DE CEMENTOS DE BRUSHITA (página 113-121)