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6.2. Diseño de la redistribución de planta

6.2.4. Evaluación de las propuestas de redistribución

]'lute 1 5 .

Plate 1 6 ,

'l'he har.d SlJr::cy 2 pp<Jratus ·.1i th ooo:� 2.nd nro t e c tive baffl e s u s e d t o :1::ply Lu:.(;'icici�s t o th:- : _ r-:�' t:reated plo t s .

Petri ciishes of c,ro�.J.nd. c <::.1) sule sho•

..

:in�· [TO\It!l o f

( 1 )

llelninthosporium,

( 2)

Alt er::1aria,

( 3 )

Clados porium,

(4)

l11oi s t contro l ,

(

5

)

Dry contro l ,

108.

6.2.3. �ascussio�

It is quite clear frot'l th-J data giver.

in

T�ble

41 that

both t:.e .: 1 tarr.aria E and the ::el;::inthos.•or:.um .2,2 :'ur1,;i dr�ticai.ly reduceC.

capsule mo�hine to less that one t

e

nth

of the

level

in the

dry, ��invculated

contr

o

lR. .Uso the isolation of

Altern'-"!"h

.:!.£ wd .§E. from the seed conf:..r:ned tr.e visual evidance of

t

he

microscopic observation (?h•te

14)

that fungi do_per.etrate and disrupt the ce�lular structure �,c enter the interior of th� capsule .

'l'�£. fact that Clados-co::-ium did not reduce morphine concentr2.tion significr,ntly belO\v tr.c:.t of t

h

e dry unir10culated control rna:;

be

a re!'l ection of the ex:;>erirr.enta

l

::;etl!od us

ed.

had a sloH

ra.te o!.' lc.tc::::-1 spread on

the

surface of the ground

ca

psule ar.d sven after 24 daJ·s diu not co::�pletel.f cove::.- the suriaoe. 'fhe

high

I:".

ois

t

ure

level of the capsule :�e�i·.;m ca;-t

l:ave

iP-1--ti. bited its s-,,re?.d r.r:C. thus li::ite

d

t!":e

po

s:::i

b

il

i

ty

of

r�vr;,hi r:a bre<kC.cwr.. On this c>s)?ect ?o:!.lsti!d

(1966)

re.:orted th;:t aer

a

tion <J<l!? a vi tal fac �e>r in

the

c,--:::.-o,;th

of Cl

zd

os

oori '.lr.1 herbarum

.

In addition

to tr.!

g ,

tv�s the dominant f1.m,;us c

ol

o

n

izir.s the surface of ccpsule

s

ir:

th

e

field tric>.l

at �ortl.side (

secti

o

n

2 . 4 . )

ar:d one of the

t.;o

rep

o

rt

ed

by lr..iczulska

( 1967)

as bein.1

associated

·,:1 th

t:.or�,hine losses

i::l :'olish ex; cr­

iments .

A

po

int of so.:1e

ilr._;Jor':;er:ce in this ex_,erin:ent

was the fact -�;::::t t

he moist, uninoculated

cor:trol 1�e.s si&-nii'icantl:r lo;.1ar in mor;:hir.c th�..n

t·'!e

dry

uninocul;.ted

ca?sule J:ii"terial (.Table 4 1 ) .

This

may imyly sorr.

e

f�r::� o: hydrolytic breakcown of corphine and

can ce

co. r

p

ared "i th th� obse�a.1:icn c:

Kopp ( 1957)

th:t harvested ca,sules stored i:l a

!::oist c>.t.rosphere s!-.o·.>ed

significant red

u

ction i

n

morphine

c

ont

e

nt

.

6.

3. T'ne Asso

c

iot

i

o

n beb1een

:'Ul�··Cll Co.lor.i

s

atio

n

-:-r.cl :

-

.or-ohxr.e Conce!"­ tration of

Intact

caosu!es.

6.

3 . 1 .

?nd I ethods

leYels \�as studied

in

a

2 x 2

factorial at Forthside Vegetable :lef:earch

3tation. T!le p'iots

•,;ere

drilled

3rd October 1971

'Nith

t-:o

= 2ero , a11d ?!1

200

kg/ha �� as

an.:noniuru

suJ.pho.te. Concentrated supcrphc·sph.:.te �1as ap;:lied

to give PO � zero and

P1

=

200

kJ/ha

P.

All treatrr:ents had contact

P

applied ·

.

.;i th the seed

to

,;i ve 2<: k�/h::!.

P.

A 1'asnl<'-''1ian cultivar was d:.-.:lled

in

200

;m rows cnti tl:e plot size 'lias e . :; :nelres

lone-

and

1 , 6

rnetr<.:s v:ice.

The plots were laid out in a randornised block ·.oli th

four

replications. At

harvest or. t:::e

28th

Februa17

1972

the cou.b\ned ter..nir:al �nd lateral ca!-•£Ules

Here di vid�d

irao

thre

e

sue-plot catetS"ories cie;lending on the perce:1ta:;·e

of

the total :::urf..:ce ar:?o. wh

i

ch •·tas colonised

by

funbi. The thr�e

catee;ories

•11ere judc1ei

by eye

as folloHs :- Sl

i

esht = Q-10;�, I<:edium =

1 1-30;�.

�ev�re "'

30;�.

6. ), 2.

l�esul ts t

,s t:1ere �1ere

nc intcr�ctior.s

betw�en fertiliser treat:..ent ��d !rfecti0n cr.tesorie3 tte oo=phir.e

conce�tratic�s

of

C?vsules

are set

out

ir.

:Cable 43 as the :.1eans of tne

four

fertiliser treatment for each infection category.

T?.ble The e::ect of level of

!r:fection

on the a.e�n �or�hine contnnt nf intact

c

acs:.�'!es

Infection :ate5cry

Jotorphine (%-)

Slight

1.)4

l'·:edi•.un

1 . 1 9

::>evere

1 . 07

:..s.D.

p <. 0.05

0.07

P <. 0.01

0.09

Table 44 records 1;he pcrce�tage of the -total

num'oer

of cc.psules f:er plot which were

in

the severe infection catesory.

1 10 .

'!'able

44.

The effect of fe

rt

j

lizer

t

r

e

at

�:en

t

on the nr·ouortion of tot'll

nu:nher of C<l:'l�ules 'Cer ':llot in the: severe i!'1f�ct

ion

Po

P1

Eeru1

1-�o

8.27

5.30

6.79

N1

12.88

21.55

17.21

!·!e

a

n

10.57

1 ) . 42

• r,. s.n. \·iitn!.n Tab:e p (

0.05

4.95

p < o. 01

c

7 . 1 2

l>:arginal

means

p <..0.05

:;. 51

p <.

0,01

=

5.04

6.;.:;.

Dis

c

ussion