Otras recomendaciones para aumentar la participación en la huerta

1

. . . ... :· .; : . ,, .. - ·. · . ·. '. � . - . . ... , . - .. .... ...

.. . .

..

2

3

+

that rnu c o prot e i ns had :pa.s s e d t h roue;h the c o lumn

uno bst n:tct e d . rrhe abs en c e of rennet ac�j i v i t y

demonst rg,t e rl that all th e re m1et was

a'thwrbed

on t o t h e

i o n ex change re sin .

4 5

Renn e t Remwt was e lut e d from the _{e: oh.JJnn} wi th

3600

l o f s t ar t in .s; buffe r made

1 -H

with

sodiwn ch l o ri de . The e lu t i on pRt t a rn was moni t ore d fro m th e t ime the e J

..

u t. i on buffer was app l i e d. t o t;b e c o lumn by rn ea[n).ring the e luent abs o rbance at 200 nm anc1. the flo·.v ra-t e ave rage d

8?0

1/h

during e lut i on .

The pat t e rn o f e l ut i on of renne t from the i o n exchan ce c o hv-,n

( Fie;ure 6 )

ind i c a·tj e s that for the fi l,f�t

1 . 4

hourf3 th <� s t ar t i ne; bttff e r , c on t a i ning so·.., e "'UC O ­

prot eins , vvas c1i spJ.A.c e d fro)n t h e c o lU'11ll by the e lut i o n buffe r . At 1 .

5

h ours t h e abso rbanc e a t 2 8 0 D"l beg;::�_n t o i n c r e as e indi c a t i ng that renn.et was pre s e nt i n the e 1u.ent . 1l1he e luent was c o l l e c t l':! ti fer

4

h ours at wh i ch t i m e the

e luent act iv i t y h ad dro ppe d t o le s s tb an

1 .

0

RTJj:�,L

In all

3 200

1

of

e luent

were c o l l e c t e d rvh i ch

contained

2 2 . 0 RU

/

r;l l e;i

ving

an increase in c o n c en.t r-at i on o f

1 .

4 5 t; itile s

·the

o :r·i gina1 a c � i

v i

t :{ . The eluent was fur·t; h e r c o nc.: en­ t rat e d imiYl e di a t r. ly bJ ult raf i l·t rat i on and s f; s.bi l i s e d by ad just ment t o

1 90 e/1

o f sodium ch loride and

7. 5 e/1

o f sodium benzO[-'t.t e . At th i s s t age t h e re were

231

1 of rennet

at

298

RU

/

m l wh ich was

97. 5%

o f t h e rennet act ivi ty that was appl i e d t o the i on exchange c o lv.Jrln .

Puri fi e d renne t . The rennet o bt aine d aft e r

purifi c at i on by i on ex change chromat o e;ra.phy was spark l ine

c l ear and has remained

so for 1 2

mont h s s i n c e preparat i on .

Th e degre e of pur i f i c at i on obtained by c om m e rc i al i on

ex chane;e chror12,t o graphy was de:n onst rat e d , as shown in

Figure

7 ,

b�r th e laborat o�J i on ex change chromat o graphy on v'Jhat m an DE 5 2 c e l lu lo s e o f t raclit ional

(

Fi gure

7 A )

and purifie d

(

Fi gv.re

7

B)

rennet s . Th e m i lk c oagulat i ng act i v i t y was e lut e d i n both c a s e s a

.. t

c onduc t i vi t i e s o f

1 1 5

5

4

2: = c::>

3

CD ('..J LJ_J L.) =

₂

<:( m er: c::> t.n m <(

0

0

2

3

T I M E

( H O U R S )

4

4 6

5

and 2 1 5 m S Hnd were rennin and p e p s i n respe ct ive ly

( O ' Le ary & Fox , 1 974 ) .

The

� ain d i fference between the

t wo chro"l a t o craph s was the s i z e of the f i rst; peak 'Nh i ch i n both c a s e s was e lut e d at the v o i d vo lu0 e of the

c o lu: � n a rld c o·n_t a i n e d the rTI)_c o prot r:: ins . In th e

t racli t i onal re nnet th i f:: first pen.k \'iHS :11UCh l8.rge r than the rennin pe ak but in the lJl.lY i :li e rl re nne f; the rennin peak was seve ral t im e s laree r than th e first peak . Thus re l a t ive t o the s i z e of t h e rennin peak th e puri f i e d rennet c ont a i n e d much l e s s o th e r mat erial th11.n �l1 e

t radi t i onal rennet . The rennet puri f i e d by i on ex change chrory1at ograr1h�r has be en at l e as t a s st c.l..ble as th e

t radi t i onal produ c t in that renne t ac t i vity l o s s has

('\ been l e s s than 1 � per m onth for 1 2 months at 4 - C .

In document La agricultura urbana como herramienta para el bienestar de las personas mayores del Centro de Protección Social Bosque Popular de Bogotá (página 104-108)