Reconoce y aplica los ritmos y

The continuity in the development of the system as well as of the data processing software would be the next steps in this project.

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The first step needed to get better results should be changing the spectrometer. The one used until now have some important flaws that are hardly solved. Ocean optics has a wide offer on spectrometers that can be used with the already existent LabVIEW program.

Find a method to improve the arrangement of the optics, in order to avoid drawbacks such as the one on the calibration procedure.

Change the position of the lenses to perform a magnification of the sample, in order to reduce the actual area covered by the pinhole, in order to increase resolution. For this task, the lens should be place nearer the sample and further from the pinhole.

Perform some scanning of a non-self-illuminated surface, using an external source of illumination.

Perform, first in vitro, and afterwards in vitro tests that show the efficiency of the technique.

A possible future approach could be the arrangement of a motor below the sample to study that works together with the galvo mirrors and spectrometer, allowing us to obtain 3-dimensional images of our sample in the hyperspectral range.

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Project Costs

The budget of the project was calculated taking into account three types of resources: Hyperspectral system components, technical equipment and human resources.

Hyperspectral system components Quantity Costs/Units Total Cost

GVS012 - 2D Large Beam (10 mm) Diameter Galvo System, Silver-Coated Mirrors. Thorlabs inc,

Delaware, USA. 1 2,538.10 € 2,538.10 €

GPS011 - 1D or 2D Galvo System Linear Power

Supply. Thorlabs inc, Delaware, USA. 1 405.00 € 405.00 €

GCM012 - 30 mm Cage Adapter for 10 mm Galvo

System. Thorlabs inc, Delaware, USA. 1 162.00 € 162.00 €

AC254-040-A-ML - f=40 mm, Ø1" Achromatic Doublet, SM1-Threaded Mount, ARC: 400-700 nm.

Thorlabs inc, Delaware, USA. 1 86.31 € 86.31 €

AC254-030-A-ML - f=30 mm, Ø1" Achromatic Doublet, SM1-Threaded Mount, ARC: 400-700 nm.

Thorlabs inc, Delaware, USA. 1 91.80 € 91.80 €

LJ1695RM-A - f = 50 mm, Ø1", N-BK7 Mounted Plano-Convex Round Cyl Lens, ARC 350-700. Thorlabs

inc, Delaware, USA. 1 89.10 € 89.10 €

SM1D12 - SM1 Lever-Actuated Iris Diaphragm (Ø0.8 -

Ø12 mm). Thorlabs inc, Delaware, USA. 1 50.13 € 50.13 €

CP02/M - SM1-Threaded 30 mm Cage Plate, 0.35" Thick, 2 Retaining Rings, M4 Tap. Thorlabs inc,

Delaware, USA. 2 14.40 € 28.80 €

CXY1Q - 30 mm Cage System, XY Translating Mount for Ø1" Optics with Quick Release Plate. Thorlabs inc,

Delaware, USA. 1 202.50 € 202.50 €

USB-600914-Bit, 48 kS/s Low-Cost Multifunction

DAQ. National Instruments Corporation, Texas, USA. 1 395.00 € 395.00 €

DH-2000 Deuterium-Halogen Light Source. Ocean

Optics Inc, Florida, USA. 3,200.00 € 3,200.00 €

Spectrometer: Spark-VIS. Ocean Optics Inc, Florida,

USA. 1 415 € 415 €

Total= 7,663.74 € Table 3. HSI system components costs.

Technical equipment Quantity Costs/Units Total Cost

OmniDriver, Cross-Platform Full-Featured Device

Driver. Ocean Optics Inc, Florida, USA. 1 500.00 € 500.00 €

LabVIEW Software (free student version).

National Instruments Corporation, Texas, USA. 1 600.00 € 600.00 €

Matlab Softwar. The Mathworks Inc,

Masschusetts, USA 1 500.00 € 500.00 €

Total= 1,600.00 € Table 4. Technical equipment costs.

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Human resources costs comprise the salaries of the team members working on the project. In this case, the student, the tutor and the Co-tutor worked full. I consider an average salary for the student of 25€/hour and an average salary for the tutor and co-tutor as engineer of 40€/hour.

Human resources Months Cost/month Total

Student 8 750 € 6,000 € Tutor 8 1,200 € 9,600 € Co-tutor 8 1,200 € 9,600 € Total= 25,200 € Table 5. Human resources costs

Concept Total cost

Hyperspectral system components 7,663.74 €

Technical equipment 1,600.00 €

Human resources 25,200 €

Total= 34,463.74 € Table 6. Total costs.

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ANNEX

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Figure 52. Terminal window of galvo_mirror_main LabVIEW program

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Figure 54. Terminal window of galvo_mirrors_scan LabVIEW program

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Figure 56. Terminal window of galvo_mirrors_scan LabVIEW program

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