Jag1 ;Nfatc1- with females Jag1 . Lethality phase of Jagged1;Nfatc1-Cre embryos. Genotypes obtained after breeding males Supplemental Table S1: Lethality phases 1

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Lethality phase of Jagged1;Nfatc1-Cre embryos. Genotypes obtained after breeding males Jag1^flox/+;Nfatc1-^Cre/+ with females

Jag1

#total

Jag1^flox/flox;Nfatc1-Cre Jag1^flox/flox;Nfatc1^+/+ Jag1^flox/+; Nfatc1-Cre Jag1^flox/+;Nfatc1^+/+

E9.5 59(10) 14 (23,73%) 11 (18,64%) 10 (16,95%) 24 (40,68%)

E10.5 28(3) 4 (14,29%) 5 (17,86%) 10 (35,71%) 9 (32,14%)

E11.5 77(21) 13 (16,88%) 22 (28,57%) 17 (22,08%) 25 (32,471%)

E12.5 134(36) 21 (15,67%) 40 (29,85%) 41 (30,60%) 52 (38,81%)

E13.5 258(65) 12 (4,65%) 86 (33,33%) 79 (30,62%) 81 (31,40%)

E14.5 90(25) 0 (0%) 28 (31,11%) 25 (27,77%) 37 (41,11%)

E15.5 22(9) 0 (0%) 8 (36,36%) 6 (27,27%) 8 (36,36%)

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Dll4

#total Dll4^flox/flox;Nfatc^+/+ Dll4^flox/

+;Nfatc^+/+

Dll4^flox/flox; Nfatc1-Cre Dll4^flox/+; Nfatc1-Cre

E9.5 292 73 (25.00%) 70 (23,97%) 86 (29,45%) 63 (21,58%)

E10.5 42 9 (21,43%) 7 (16,67%) 12 (28,57%) 14 (33,33%)

E11.5 43 6 (13,95%) 9 (20,93%) 14 (32,56%) 14 (32,56%)

E12.5 233 34 (14,59%) 65 ( 27,90%) 64 (27,47%) 70 (30.04%)

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Dll4

#total

Dll4^flox/flox;Cdh5-Cre

Dll4^flox/flox; Cdh5^+/+

Dll4^flox/+; Cdh5-Cre

Dll4^flox/+; Cdh5^+/+

E14.5 4 1 (25.00%) 0 (0%) 1 (25%) 2 (25%)

E15.5 321 47 (14.64%) 93 (28.97%) 86 (26.79%) 95 (29.60%)

E16.5 53 6 (11.32%) 20 (37.74%) 13 (24.53%) 14 (26.42%)

males ManicFringe^Gof/+;Tie2-^Cre/+ with femalesManicFringe^Gof/Gof

#total

ManicFringe^Gof/Gof;

Tie2-Cre ManicFringe^Gof/Gof; Tie2^+/+

ManicFringe^Gof/+;

Tie2-Cre ManicFringe^+/+; Tie2^+/+

E13.5 46 13 (25.8%) 11 (23.91%) 9 (19.57%) 13 (28.26%)

E14-E15.5 225 48 (21.33%) 60 (26.67%) 63 (28.00%) 54 (24.00%)

E16.5 442 85 (19.23%) 110 (24.89%) 113 (25.57%) 134 (30.32%)

P1 44 4 (9.09%) 20 (45.45%) 7 (15.91%) 13 (29.55%)

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Efnb2

#total

Efnb2^flox/flox;Nfatc1-Cre EfnB2^flox/flox;

Nfatc1^+/+ Efnb2^flox/+;Nfatc1-Cre Efnb2^flox/+;

Nfatc1^+/+

E9.5 14 (2) 3 (21.4%) 4 (18.7%) 3 (21.4%) 4 (28.6%)

E11.5 8 (1) 2 (25.0%) 3 (37.5%) 1 (12.5%) 2 (25.0%)

E12.5 11 (2) 2 (18.2%) 2 (11.0%) 5 (45.5%) 2 (18.2%)

E13.5 67 (9) 12 (17.9%) 16 (23.9%) 13 (19.4%) 26 (38.8%)

E14.5 62 (9) 16 (25.8%) 18 (29.0%) 11 (17.7%) 17 (27.4%)

E15.5 245 (31) 57(23.3%) 60 (24.5%) 62 (25.3%) 66 (26.9%)

E16.5 379 (53) 86 (22.7%) 101 (26.6%) 99 (26.1%) 93 (24.5%)

E17.5

E18.5 178 (23) 33 (18.5%) 55 (30.9%) 40 (22.5%) 50 (28.1%)

P1 142 (20) 23 (16.2%) 35 (24.6%) 42 (29.6%) 42 (29.6%)

P21 119 0 (0%) 35 42 42