[PDF] Top 20 diseño y contruccion de un quemador
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Metabolomic basis of laboratory evolution of butanol tolerance in photosynthetic Synechocystis sp. PCC 6803
... LC-MS metabolomic analysis was also ap- plied to characterize changes in the cyanobacterial primary metabolism under diverse environmental conditions or in defined ...of Synechocystis (Additional file 1: ... See full document
158
Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution
... of Synechocystis sp. PCC 6803 were obtained via the ALE method using medium containing isobutanol, which induces strong ...strated tolerance against a wide variety of alcohols, such as ... See full document
143
Integrated OMICS guided engineering of biofuel butanol-tolerance in photosynthetic Synechocystissp. PCC 6803
... enhanced tolerance to n-butanol in ...synergistic tolerance mechanism, genome-wide based analytical approaches are necessary ...of Synechocystis sp. PCC 6803 (hereafter ... See full document
16
Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803
... in Synechocystis as discussed before by Lindberg et ...that photosynthetic carbon is primarily converted into sugar (80–85 %), whereas syn- thesis of fatty acids (~10 %) and terpenoids (3–5 %) lag far ... See full document
25
Shao, Lin (2012): Characterization of the TPR protein family and a putative photosynthetic protein from Synechocystis PCC 6803. Dissertation, LMU München: Fakultät für Biologie
... in Synechocystis 6803, several TPR proteins have been found to be involved in this process, e,g, PratA which interacts directly with D1 and participates in D1 processing (Klinkert et ...from ... See full document
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Synechocystis KaiC3 Displays Temperature- and KaiB-Dependent ATPase Activity and Is Important for Growth in Darkness
... the Synechocystis wild type, the kaiAB1C1 deletion mutant (ΔkaiAB1C1), and the kaiC3 deletion mutant (ΔkaiC3) was tested under mixotrophic conditions in continuous light and under heterotrophic dark ...the ... See full document
31
Re-direction of carbon flux to key precursor malonyl-CoA via artificial small RNAs in photosynthetic Synechocystis sp. PCC 6803
... doxorubicin [18, 34], and thus, the enhancing cellular pool of malonyl-CoA could potentially benefit the pro- duction for malonyl-CoA-derived products. The pre- vious studies have suggested fatty acid biosynthesis as the ... See full document
32
The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism
... the Synechocystis acetate transporter expres- sion strain (AT) was evaluated in comparison to the con- trol strain (CS) harboring a corresponding empty plasmid under different intensities of constant light, in the ... See full document
102
Conserved Residues in Ycf54 are required for Protochlorophyllide Formation in Synechocystis sp. PCC 6803.
... HNCI/[eh76"cpf"HNCI/C;I0"Kpfggf."rtgxkqwu"rwnnfqyp"gzrgtkogpvu"*3;+"ujqygf"vjcv"qpn{"HNCI/ [eh76"nqecvgf"ykvjkp"vjg"kpuqnwdng"htcevkqp"ycu"ecrcdng"qh"kpvgtcevkpi"ykvj"E{eK"cpf"cpcn{ugu"q[r] ... See full document
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A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803
... limitation, so called marker-less strategies have been de- veloped, which allow for the removal of the integrated marker. The first marker-less method was established in Gram-negative bacteria using the nptI-sacB double ... See full document
13
Characterisation of the slr1212 genomic region of the freshwater cyanobacterium Synechocystis sp PCC 6803
... SIr1212 possesses homology to i the binding domain of ethylene receptors of higher plants, ii PAS/P AC domains, potentially involved in ligand binding and protein dimerisation, iii GAF d[r] ... See full document
168
Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803
... and metabolomic analysis and their relevance to 3-HP production, attempt was made to utilize the informa- tion for further modifying 3-HP-producing SM ...bestow tolerance and often improve production in ... See full document
20
Cloning and expression of two genes (NHAS2 AND NHAS4) isolated from SYNECHOCYSTIS SP PCC 6803 in yeast
... Synechocystis sp. PCC 6803 was the first cyanobacteria to have its genome sequenced in 1996 (Nakao et ...strain. Synechocystis sp. PCC 6803 has revealed a ... See full document
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Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803
... in Synechocystis 6803, including pro- moters, RBS, and terminators using a standardized genetic setting for ...utilizing Synechocystis 6803 as an efficient autotrophic green factory for bio- ... See full document
16
Identification and Characterization of Enzymes Involved in Post-translational Modifications of Phycobiliproteins in the Cyanobacterium Synechocystis sp. PCC 6803
... All PCR amplification reactions were performed with 40 pmol of each primer, 10 ng of Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002 chromosomal DNA, and 25 µl of PCR ... See full document
20
Expanding the toolbox for Synechocystis sp PCC 6803 : validation of replicative vectors and characterization of a novel set of promoters
... considered the ‘green’ Escherichia coli, is the best studied cyanobacterium but to be used as an efficient and robust photoau- totrophic chassis it requires a customized and well-characterized toolbox. In this context, ... See full document
10
A study of the impact of mazie SBE I on the [alpha] polyglucan produced in Synechocystis sp strain PCC 6803
... Protein was extracted as described in Chapter 2. A 7.5 % SDS-PAGE resolving gel (15 cm x 10 cm x 0.15 cm) was used to electrophoretically separate 25 µg of total soluble proteins from WT, M3 and SM3, and 1 µg of total ... See full document
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The alternative sigma factor SigF is a key player in the control of secretion mechanisms in Synechocystis sp. PCC 6803.
... in Synechocystis sp. PCC 6803 physiology, with a major impact on growth and secretion mechanisms, such as the production of extracellular polysaccharides, vesiculation and protein ... See full document
403
Proteomic analysis reveals resistance mechanism against biofuel hexane in Synechocystis sp. PCC 6803
... iTRAQ - LC-MS/MS technologies to reveal the responses of Synechocystis to hexane, a representative of alkane. Using a cutoff of 1.5-fold change and a p-value less than 0.05, a total of 164 up-regulated and 77 ... See full document
82
Identification and bioinformatic analysis of the membrane proteins of synechocystis sp. PCC 6803
... photosynthetic thylakoid membrane. Seventy-two, 29, and 76 proteins have been identified from each purified membrane by several consecutive studies [3-5,8], respec- tively. Because we did not separate the ... See full document
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