[PDF] Top 20 La entrevista no estructurada en investigación cualitativa: una experiencia de campo
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Implementation of a CRISPR-Based System for Gene Regulation in Candida albicans
... in stationary phase but reached approximately 10 ⫻ to 13 ⫻ during the log phase of growth (Fig. 3C), demonstrating that activation was increased when different activator modules were recruited (Gal4 plus VP64). Studies ... See full document
183
A CRISPR Interference Platform for Efficient Genetic Repression in Candida albicans
... CRISPRi system for C. albicans. Initially, to develop a CRISPRi system for genetic repression in ...C. albicans, we generated a nuclease-dead version of Cas9 (dCas9), optimized for use in ... See full document
136
Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species
... plasmid system for gene editing in ...pCP-tRNA system, except that different regulatory elements and a different autonomously replicating sequence are ...SAT1 gene (nourseothricin resistance) ... See full document
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Marker Recycling in Candida albicans through CRISPR-Cas9-Induced Marker Excision
... upon CRISPR-Cas9 sys- ...C. albicans for proof of principle. C. albicans is extremely important clinically (7) and presents challenging genetics because it is naturally diploid and lacks a complete ... See full document
113
Ssn6 Defines a New Level of Regulation of White Opaque Switching in Candida albicans and Is Required For the Stochasticity of the Switch
... expression of about 20% of the protein coding genes in the C. albicans FIG 4 The white and opaque cell regulatory networks as determined by ChIP-chip analysis. The core regulatory networks in white (a) and opaque ... See full document
73
Tetracycline-Inducible Gene Expression and Gene Deletion in Candida albicans
... a system that enables an inducible expression of target genes in ...C. albicans cells by a small-molecule inducer, doxycycline, independently of the growth ...regulatable gene expression ... See full document
36
Developmental Regulation of an Adhesin Gene during Cellular Morphogenesis in the Fungal Pathogen Candida albicans
... the regulation of a second hypha-specific gene, ...C. albicans responds to an ex- tremely disparate range of environmental conditions by form- ing hyphae ...C. albicans may be affected by ... See full document
10
Multicenter Evaluation of Candida QuickFISH BC for Identification of Candida Species Directly from Blood Culture Bottles
... the Candida QuickFISH BC assay include the detection of only three yeast species (although this encompasses the most commonly encountered Candida species), the relatively high cost of the assay, and the ... See full document
82
Synthesis and biological evaluation of some 4 hydroxy 3 [3 (substituted phenyl) prop 2 enoyl] 2h chromen 2 ones
... Aspergillusfumigatus, Candida albicans, Candida albicans ATCC 10231, Candida krusei G03, Candida glabrata H05 were employed for testing antifungal activity using well ... See full document
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Lower filamentation rates of Candida dubliniensis contribute to its lower virulence in comparison with Candida albicans
... Keywords: Candida albicans, Candida dubliniensis, hyphae, morphogenesis, infection 17 models, virulence 18 19.. Candida albicans is routinely reported as the most common cause of superfi[r] ... See full document
125
A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus
... CRISPRi system, we measured the timing of CtrA and GcrA depletion in a mixed, asynchronous population of cells using sgRNAs specific to each transcript targeting the nontemplate strand ...CRISPRi system ... See full document
98
Risk factors for fatal candidemia caused by Candida albicans and non albicans Candida species
... by Candida species have increased ...years, Candida spe- cies associated with candidemia have shifted from Cand- ida albicans to non-albicans Candida species (NAC) ... See full document
5
Multicenter Evaluation of the New VITEK 2 Advanced Colorimetric Yeast Identification Card
... C. albicans to non-C. albicans species ...to Candida diagnostics, they exhibit variable sen- sitivities, can be time- and labor-intensive, and may not be applicable for routine clinical use ... See full document
262
Rapid Identification of Pathogenic Fungi Directly from Cultures by Using Multiplex PCR
... C. albicans, ...of Candida dubliniensis, Candida guilli- ermondii, Candida kefyr, Candida krusei, Candida lusitaniae, Candida pichia, ... See full document
10
Rapid Identification of Candida Species with Species Specific DNA Probes
... Specificity of digoxigenin-labeled probes. Eighteen Candida species probes (Table 1) were designed and tested in the PCR- EIA against the DNAs from the fungi listed in Table 2. The absorbance value obtained for ... See full document
18
Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry for Fast and Reliable Identification of Clinical Yeast Isolates
... of Candida (n ⴝ 250), Cryptococcus, Saccharomyces, Trichosporon, Geotrichum, Pichia, and Blastoschizomyces ...32C system (bioMe ´rieux, Nu ¨rtingen, ...diagnostic system identified 244 isolates in ... See full document
157
MAP Kinase Regulation of the Candida albicans Pheromone Pathway
... C. albicans establishes that the MAPK duplication preceded the WGD, occurring coinci- dentally with the appearance of an Ste5 equivalent ...C. albicans mating pathway (reference 12 and this ... See full document
9
Pseudohyphal Regulation by the Transcription Factor Rfg1p in Candida albicans
... HSL1 gene, which encodes a septin-associated mitotic ki- nase, produces a strain which when grown under yeast con- ditions forms pseudohyphae at low cell density and grows as yeast cells at high ... See full document
143
Expression of the Candida albicans Morphogenesis Regulator Gene CZF1 and Its Regulation by Efg1p and Czf1p
... growth phase. CZF1 was expressed most strongly in post-ex- ponential-phase cells grown in glucose-containing medium at 37°C. This regulation suggests that CZF1 would be expressed by C. albicans cells ... See full document
240
Infectious diseases detection by microarray: An overview of clinical relevant infections
... relevant system was designed by Gryadunov et ...rpoB gene responsible for rifampicin resistance as well as 11 muta- tions in the katG gene, five mutations in the promoter ... See full document
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