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Biotechnological Uses of Spider Silk
... of spider silk that can be created, depending on species and environment: Major Ampullate Silk, Flagelliform Silk, Aggregate Silk, Minor Ampullate Silk, Pyriform Silk, ... See full document
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Study of Spider Silk Fibers by Raman Microscopy
... Raman spectroscopy is a technique used to study the normal modes of oscilla- tion of the chemical structures that compose materials; vibrational and rotation- al modes are found within the frequencies. The technique ... See full document
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Evidence for antimicrobial activity associated with common house spider silk
... recent biotechnological applications of spider silk includes the use of recombinant spider silk particles as drug delivery vehicles ...[18]. Spider silk has also been ... See full document
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Hofer, Markus (2013): Development of spider silk protein particles for pharmaceutical applications. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... obtained spider silk suspensions are shown in Figure ...unloaded spider silk particles appear at pH 7 as dark spots upon strong green fluorescence caused by the unbound FITC-dextran in ... See full document
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Stretching of supercontracted fibers: a link between spinning and the variability of spider silk
... of spider fibers, as a central feature of the spinning ...of silk production, when the silk exits the spigot but is likely to remain hydrated for a short period of time, would lead to the observed ... See full document
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Prey type, vibrations and handling interactively influence spider silk expression
... MaSp1 has more abundant poly-A and GGX motifs, so alterations in the relative ratios of MaSp1 and MaSp2 may be detected via alanine and glycine concentrations, higher concentrations of either amino acid representing ... See full document
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Structure and Strength: Anisotropic Polyvinyl Alcohol Hydrogels and Spider Mite Silk Fibres
... Typical force versus displacement curves exhibit non-linear behaviour and a range of initial slopes as shown in Fig. 5.5. Though the force versus displacement curves are non-linear, agreement between the data and the ... See full document
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Evolution of supercontraction in spider silk: structure–function relationship from tarantulas to orb weavers
... of silk properties during fiber ...duct, silk is initially in a supercontracted state and is thus as compliant as ...the silk thread during spinning controls molecular orientation and hence, the ... See full document
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Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
... of spider silk protein composite nanofibrous scaffolds, so as to get bionic extracellular matrix three-dimensional porous scaffolds with even fiber diameter (300–500 nm) and smooth ... See full document
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<p>Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16)</p>
... charged spider silk fi lms and these were shown to retain good bactericidal ef fi cacy and overcome the issue of low particle stability in culture ...the spider silk protein ... See full document
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Bioengineering the spider silk sequence to modify its affinity for drugs
... Results: The bioengineered silk variant, EMS2, was constructed, produced, and purified. The EMS2 silk retained the self-assembly property and formed spheres. The spheres made of EMS2 and MS2 silks were not ... See full document
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Quantification of the physiochemical constraints on the export of spider silk proteins by Salmonella type III secretion
... In this manuscript, we have used a set of spider silk polypeptides to characterize the physiochemical proper- ties that limit the use of the SPI-1 T3SS for the export of recombinant proteins. The most ... See full document
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Quasistatic and continuous dynamic characterization of the mechanical properties of silk from the cobweb of the black widow spider Latrodectus hesperus
... the silk used to construct the gumfooted capture threads of cobwebs has been uncertain and hypotheses have included major ampullate, minor ampullate, and flagelliform glands (Benjamin and Zschokke, 2002; ... See full document
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Silken toolkits: biomechanics of silk fibers spun by the orb web spider Argiope argentata (Fabricius 1775)
... Spider silk is an inspiration for biomimetic super fibers because of the high tensile strength and unmatched toughness of major ampullate draglines (Kaplan et ...1999). Silk also provides ecologists ... See full document
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... jumping spider, no house spider or cobweb spider or wolf spider, not even the large and hairy tarantula, resembles the three deadly ...a spider and don’t see the telltale colors or ... See full document
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Giant wood spider Nephila pilipes alters silk protein in response to prey variation
... Effect of diet on dragline amino acid compositions After N. pilipes fed with mealworms built three webs, dragline collected from 20 individuals designated to fed with different prey type did not show significant ... See full document
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How super is supercontraction? Persistent versus cyclic responses to humidity in spider dragline silk
... dragline silk from five specimens of the golden silk orbweaver Nephila clavipes (Linnaeus 1776) from FL, USA and Costa ...dragline silk, we collected fibers by forcible silking and glued them across ... See full document
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Schwab, Martin (2009): Degradation of lipid based drug delivery systems and characterization of semi-synthetic spider silk proteins for the application in pharmaceutical technology. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... that silk fibroin hydrogels can serve as a rate-controlling barrier and may be used as a drug carrier system for the controlled delivery of drugs like buprenorphine ...[138]. Silk hydrogels prepared from ... See full document
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Antibiotic spider silk: site specific functionalisation of recombinant spider silk using ‘click’ chemistry
... the number of methionine codons it’s gene contains. [23,24] Cu (I) is known to be cytotoxic, is used to catalyse the ‘click’ reaction. Therefore to conserve biocompatibility, copper must not be present in the final ... See full document
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The influence of anaesthesia on the tensile properties of spider silk
... the spider (Garrido et ...a spider with a body weight of 10·mN ...the spider lacks the ability to sustain such low forces for longer than a few ... See full document
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