[PDF] Top 20 ¿Ha sido estable el crecimiento en América Latina?
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Tetracycline-Inducible Gene Expression and Gene Deletion in Candida albicans
... -M2 gene contained in plasmid pUHrT62-1 (44) showed that in addition to the amino acid exchanges that created rtTA-M2 a total of 19 CTG codons, which would be mistranslated as serine instead of leucine in ...C. ... See full document
36
Candida albicans Gene Deletion with a Transient CRISPR-Cas9 System
... CRISPR-associated gene 9 (CRISPR-Cas9) systems are used for a wide array of genome-editing applications in organisms ranging from fungi to plants and ani- ...pathogen Candida albicans; the system ... See full document
19
Strains and Strategies for Large-Scale Gene Deletion Studies of the Diploid Human Fungal Pathogen Candida albicans
... prevailing gene disruption methods, which are typi- cally carried out with long PCR primers with 60 to 80 nucleo- tides of ...C. albicans alleles of a given gene are often heterolo- gous in sequence ... See full document
5
Implementation of a CRISPR-Based System for Gene Regulation in Candida albicans
... Candida albicans is a clinically relevant diploid pathogenic fungus that is commonly found as a harmless commensal of humans but which is able to cause severe diseases among immunocompromised ...C. ... See full document
183
Deletion of a Yci1 Domain Protein of Candida albicans Allows Homothallic Mating in MTL Heterozygous Cells
... for gene expression under the glucose incubation con- ditions, and we compared these microarray data with those of the MTLa/a wild-type opaque/white comparison from the literature (31, ...the ... See full document
63
N-Acetylglucosamine (GlcNAc)-Inducible Gene GIG2 Is a Novel Component of GlcNAc Metabolism in Candida albicans
... catabolic gene expression irre- spective of GlcNAc ...regulate gene expression in the overall process of GlcNAc ...from expression analysis of an hxk1 mutant compared with the wild type ... See full document
139
Neddylation and CAND1 Independently Stimulate SCF Ubiquitin Ligase Activity in Candida albicans
... neddylation-dissociated). Expression of the Candida albicans CAND1 gene homolog CaTIP120 in Saccharomyces cerevisiae is toxic only in the presence of CaCdc53, consistent with a specific ... See full document
6
Roles of TUP1 in Switching, Phase Maintenance, and Phase-Specific Gene Expression in Candida albicans
... suppress gene expres- sion, and we assume that in C. albicans its function is ...suppress expression of one or more genes involved in the switch event(s) through the recruitment of deacetylase ... See full document
9
Transcript profiling reveals rewiring of iron assimilation gene expression in Candida albicans and C dubliniensis
... reduced growth of the nrg1 Δ mutant at pH 4.5 with bovine haemoglobin (0.1 µM and 1 µM) as the sole iron source relative to wild-type (Fig. 3b). Similarly, growth was restored to wild-type levels at pH 6.5 (Fig. 3b). ... See full document
6
MFα1, the Gene Encoding the α Mating Pheromone of Candida albicans
... that deletion of both alleles of this gene led to sterility in MTL␣ but not MTLa strains, and return of the wild-type MF␣1 gene in the mf␣1 null mutant restored its mating ...the gene we have ... See full document
72
Unique Aspects of Gene Expression during Candida albicans Mating and Possible G1 Dependency
... 4. Candida albicans forms long conjugation tubes that superficially resemble hyphae, while Saccharomyces cerevisiae forms comparatively short evaginations upon pheromone ...C. albicans P37005 [a/a]) ... See full document
211
Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns
... C. albicans biofilm formation is initiated when planktonic cells adhere to a surface, presumably through cell wall-located adhesion molecules, and begin to aggregate into a micro- ...ALS gene family, ... See full document
12
Probiotic lactobacillus and estrogen effects on vaginal epithelial gene expression responses to Candida albicans
... Human NF-κB Signaling Pathway, PAHS-077A Human Inflammatory Response and Autoimmunity, PAHS-014A Human Signal Transduction Pathway Finder; together, they measured mRNA levels of 145 genes associated with innate immune ... See full document
7
Expression of the Candida albicans Morphogenesis Regulator Gene CZF1 and Its Regulation by Efg1p and Czf1p
... showing expression of CZF1 under various growth ...CZF1 expression normalized to ACT1 levels were ...the expression levels in lanes 4 and 5 cannot be compared quantitatively to those in lanes 1 to ... See full document
240
Deletion of the Dynein Heavy-Chain Gene DYN1 Leads to Aberrant Nuclear Positioning and Defective Hyphal Development in Candida albicans
... C. albicans, is lethal in U. maydis (23). C. albicans is one of the most important human fungal pathogens, and its ability to change from yeast to hyphal stages has been shown to contribute significantly to ... See full document
336
Real-Time Evolution of a Subtelomeric Gene Family in Candida albicans
... In Candida albicans, the telomere-associated (TLO) genes are the only widespread subtelomeric gene family (Van Het Hoog et ...most Candida species to 14 paralogs in C. albicans (Butler ... See full document
18
Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
... To achieve simultaneous multiple knock-outs in O. polymorpha, three genes OpURA3, OpHIS3 and OpLEU2 were selected as the target loci. The UHA and DHA (~ 1 kb) fragments of each target gene were joined by Splicing ... See full document
122
Repeated use of GAL1 for gene disruption in Candida albicans.
... We have explored the feasibility of using a method described by ALANI, GAO and KLECKNER (1987). In this procedure, the selectable marker is flanked by direct repeats o[r] ... See full document
12
Isogenic strain construction and gene mapping in Candida albicans.
... EcoRI digested genomic DNA form selected strains is shown in the lower panel. The position and size in kilobases of electrophoretic standards are indi- cated to the right. One a[r] ... See full document
113
Androgen receptor expression in human prostate cancer cell lines
... COS-1 cells were transiently transfected with the four AR expression vectors (pcDNA-AR, pSVARo, pCMVhAR, and pTRE-AR). Total RNA, isolated 48 hours after transfection, was reverse transcribed into cDNA which was ... See full document
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