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Human vaccination against Plasmodium vivax Duffy-binding protein induces strain-transcending antibodies
... Vaccine-induced antibodies inhibit PvDBP_RII-DARC binding in ...inhibit binding of recombinant vaccine-homologous PvDBP_RII (SalI) to its receptor (in this case the recombinant N-terminal region of ... See full document
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In vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte adapted P knowlesi A1 H 1 strain
... The antibodies also recognized apical end organelle of merozoites and fragmentation of native protein by west- ern ...that protein and antibody produc- tions in this study worked well and different ... See full document
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Genetic diversity, natural selection and haplotype grouping of Plasmodium vivax Duffy binding protein genes from eastern and western Myanmar borders
... the Plasmodium merozoite and the host red blood cell (RBC) is central to the invasion process of the ...P. vivax involves the Duffy-binding protein (PvDBP), a microneme protein, ... See full document
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Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein
... display antibodies offer a way to produce high-affinity single-chain variable fragment (scFv) derivatives of human an- tibodies of “natural host” origin ...produce human monoclonal antibodies ... See full document
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The Plasmodium vivax rhoptry neck protein 5 is expressed in the apical pole of Plasmodium vivax VCG 1 strain schizonts and binds to human reticulocytes
... by Plasmodium falciparum (and RON8 in Toxoplasma gondii), associated with a micronemal protein called apical membrane antigen 1 (AMA-1) ...monoclonal antibodies directed against PfAMA-1 and ... See full document
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Antibodies to Cryptic Epitopes in Distant Homologues Underpin a Mechanism of Heterologous Immunity between Plasmodium vivax PvDBP and Plasmodium falciparum VAR2CSA
... neutralizing antibodies. In Plasmodium falciparum, we show that a mechanism to circumvent this challenge is to elicit antibodies to cryptic epitopes that are not under immune ...that ... See full document
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Immunogenicity of a Synthetic Vaccine Based on Plasmodium vivax Duffy Binding Protein Region II
... nonprotective, strain-specific humoral immune ...a strain-specific immunodominant variant epitope normally present on DBPII ...anti-DBPII antibodies were elicited against conserved ... See full document
127
A Novel Erythrocyte Binding Protein of Plasmodium vivax Suggests an Alternate Invasion Pathway into Duffy Positive Reticulocytes
... anti-EBP2 antibodies, their potential to block EBP2-reticulocyte binding was ...of protein G-purified IgG from mouse serum raised against recombinant EBP2 prior to addition of human ... See full document
105
Design and Immunogenicity of a Novel Synthetic Antigen Based on the Ligand Domain of the Plasmodium vivax Duffy Binding Protein
... acquired human antibodies to identify dominant B-cell epitopes within DBPII that correlated with functional inhibition of the ligand domain ...cyte binding but do flank residues critical for ... See full document
154
Identification of an Immunogenic Broadly Inhibitory Surface Epitope of the Plasmodium vivax Duffy Binding Protein Ligand Domain
... The Plasmodium vivax Duffy binding protein region II (DBPII) is a vital li- gand for the parasite’s invasion of reticulocytes, thereby making this molecule an at- tractive vaccine ... See full document
122
Human antibodies that slow erythrocyte invasion potentiate malaria-neutralizing antibodies
... variant protein (Figure 3E), a finding that also repeated when using purified IgG from rabbits immunized with PfRH5FL (data not ...IgG against PfRH5Nt was previously reported in the serum of these ... See full document
193
Molecular epidemiology of Plasmodium vivax and Plasmodium falciparum malaria among Duffy positive and Duffy negative populations in Ethiopia
... the Duffy blood group antigen on the surface of their red blood cells because of the conferred resistance to certain malaria parasites ...The Duffy antigen receptor for chemokines ( DARC ), also known as Fy ... See full document
5
The effect of immunization schedule with the malaria vaccine candidate RTS,S/AS01E on protective efficacy and anti circumsporozoite protein antibody avidity in African infants
... fusion protein of HBsAg and a carboxy-terminal frag- ment of Plasmodium falciparum circumsporozoite pro- tein (CSP), co-expressed in yeast and formulated with a proprietary adjuvant ...G antibodies ... See full document
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PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
... Reticulocyte binding was tested in triplicate by flow cytom- etry and using the total cells from cord blood sample (Fya - Fyb + ...recombinant protein (PvGAMA-CR1, PvGAMA-VR1, PvGAMA-CR2 and PvGAMA-VR2) for ... See full document
5
Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia
... The Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) is an important ...this protein in many countries around the world, resulting in a truncated, but expressed ... See full document
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Erythrocyte binding assays reveal higher binding of Plasmodium knowlesi Duffy binding protein to human Fya+/b+ erythrocytes than to Fya+/b erythrocytes
... the binding level was quantita- tively determined by counting the number of rosettes in the erythrocyte binding assay, and the difference in the mean number of rosettes obtained in the assays for Fy a+/b+ ... See full document
9
Natural selection and genetic diversity of domain I of Plasmodium falciparum apical membrane antigen 1 on Bioko Island
... The World Malaria Report 2018 draws on data from 87 countries and areas with ongoing malaria transmission. These reports show that after an unprecedented period of success in global malaria control, progress has stalled ... See full document
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Schistosoma japonicum: The design and experimental evaluation of a multivalent DNA vaccine
... Schistosomiasis is a parasitic disease with a chronic debilitating character, occurring in tropical and subtropical environments. Although a lot of effort has been made to control its impact on human beings, it ... See full document
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var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1 2
... 38. Gardner MJ, Hall N, Fung E, White O, Berriman M, Hyman RW, Carlton JM, Pain A, Nelson KE, Bowman S, Paulsen IT, James K, Eisen JA, Rutherford K, Salzberg SL, Craig A, Kyes S, Chan MS, Nene V, Shallom SJ, Suh B, ... See full document
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Genetic variability and natural selection at the ligand domain of the Duffy binding protein in brazilian Plasmodium vivax populations
... P. vivax . Mero- zoite invasion of erythrocytes by P. vivax relies on an interaction between a ligand on the parasite and the Duffy antigen/receptor for chemokines (DARC) on the surface of ... See full document
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