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The Capsule of Cryptococcus neoformans Modulates Phagosomal pH through Its Acid-Base Properties
... biotin (2 mM) was added to the cell samples and incubated for 30 min at room temperature. Cells were then washed three times with PBS with 100 mM glycine to remove excess biotin reagent and by-product. After ... See full document
112
Capsule Growth in Cryptococcus neoformans Is Coordinated with Cell Cycle Progression
... hypothesis, capsule growth should stop or dimin- ish during budding, so we used time-lapse microscopy to visualize capsule ...The capsule, however, is not visible under regular conditions given ... See full document
90
Nonlytic Exocytosis of Cryptococcus neoformans from Macrophages Occurs In Vivo and Is Influenced by Phagosomal pH
... fungus Cryptococcus neoformans with macrophages is the phenomenon of nonlytic exocytosis, also referred to as “vomocytosis” or phagosome extrusion/expulsion, which involves the escape of fungal cells from ... See full document
65
Radial Mass Density, Charge, and Epitope Distribution in the Cryptococcus neoformans Capsule
... the capsule structure, dif- ferences in physical and antigenic properties were demon- ...and capsule volume of dif- ferent cryptococcal strains with various capsule sizes ...exposed ... See full document
171
Cryptococcosis Serotypes Impact Outcome and Provide Evidence of Cryptococcus neoformans Speciation
... ABSTRACT Cryptococcus neoformans is a human opportunistic fungal pathogen causing severe disseminated meningoencephali- tis, mostly in patients with cellular immune ...C. neoformans serotypes A and D ... See full document
99
Invasion of the Central Nervous System by Cryptococcus neoformans Requires a Secreted Fungal Metalloprotease
... C. neoformans’ cell surface and secreted proteins in CNS invasion (17, ...C. neoformans to cause brain ...hyaluronic acid (a product of the CPS1 gene) and the CD44 host receptor has been amply ... See full document
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Pbx Proteins in Cryptococcus neoformans Cell Wall Remodeling and Capsule Assembly
... Protein purification and localization. Protein purification was per- formed at 4°C using a previously described method (50) with some mod- ifications. Cells were grown overnight in 50 ml of YPD to late log phase, ... See full document
9
Ordered Kinetochore Assembly in the Human Pathogenic Basidiomycetous Yeast Cryptococcus neoformans
... yeast Cryptococcus neoformans, a human ...C. neoformans, the kinetochore assembles in an ordered manner prior to mitosis in concert with a partial opening of the nuclear ...C. neoformans is ... See full document
14
Role of an Expanded Inositol Transporter Repertoire in Cryptococcus neoformans Sexual Reproduction and Virulence
... ABSTRACT Cryptococcus neoformans and Cryptococcus gattii are globally distributed human fungal pathogens and the leading causes of fungal ...C. neoformans can utilize myo-inositol as a sole ... See full document
219
Chloroquine induces human mononuclear phagocytes to inhibit and kill Cryptococcus neoformans by a mechanism independent of iron deprivation
... to Cryptococcus neoformans are common in AIDS ...the pH of phagolysosomes, on the anticryptococcal activity of mononuclear ...C. neoformans multiplied within monocyte-derived macrophages (MDM) ... See full document
83
Activity of tannins from Stryphnodendron adstringens on Cryptococcus neoformans: effects on growth, capsule size and pigmentation
... molecules through non-specific forces such as hydrogen bonding to H-bond-accepting groups and hydrophobic interactions arising from the aromatic rings in their gallic acids, as well as by covalent-bond formation ... See full document
22
Microevolution of Serial Clinical Isolates of Cryptococcus neoformans var grubii and C gattii
... In this study, we tried to link phenotypic changes to genetic changes to reveal the contribution of microevolution in building pathogenicity of a microorganism within the human body. Clearly, we successfully identified ... See full document
84
Lipid Flippase Subunit Cdc50 Mediates Drug Resistance and Virulence in Cryptococcus neoformans
... ABSTRACT Cryptococcus neoformans is a human fungal pathogen and a major cause of fungal meningitis in immunocompro- mised ...C. neoformans but exert a fungistatic effect, necessitating long treatment ... See full document
66
Unraveling Melanin Biosynthesis and Signaling Networks in Cryptococcus neoformans
... Gene disruption and complementation. The gene deletion mutants used in this study are listed in Table S1. Each gene disruption cassette contained nourseothricin, G418, or hygromycin B selection marker (NAT, NEO, or HYG, ... See full document
99
Radiological Studies Reveal Radial Differences in the Architecture of the Polysaccharide Capsule of Cryptococcus neoformans
... the capsule is presumably assembled ...the capsule, it is also possible that the density and composition of the capsule are continuous, such that changes occur as a function of radial ... See full document
12
The Cryptococcus neoformans Transcriptome at the Site of Human Meningitis
... C. neoformans is metabolically active in the human ...survival properties are identified using in vivo transcrip- tion profiling, which validates this ...Fifth, through the combination of RNA-seq and ... See full document
90
Mixed Infections and In Vivo Evolution in the Human Fungal Pathogen Cryptococcus neoformans
... C. neoformans populations in the environment and suggested that their function may be to generate genetic diversity de novo during the sexual cycle ...C. neoformans causing cryptococcosis— care should be ... See full document
130
Identification of Aph1, a Phosphate Regulated, Secreted, and Vacuolar Acid Phosphatase in Cryptococcus neoformans
... Acid phosphatase Aph1 is a component of the secretome of strain H99. We identified 105 proteins in the H99 secretome by using mass spectrometry (see Table S1 in the supplemental mate- rial). This is a higher ... See full document
86
The Buoyancy of Cryptococcus neoformans Is Affected by Capsule Size
... C. neoformans cell ...C. neoformans to compare the densities of melanized and nonmelanized cultures started from the same Sab ...of capsule by gamma ...the capsule radii of melanized and ... See full document
90
Capsule Structural Heterogeneity and Antigenic Variation in Cryptococcus neoformans
... C. neoformans, we examined the expression of PS epitopes in serotype D and serotype C strains during different growth phases as revealed by MAb ...the base- line level, as seen on agar ...the capsule ... See full document
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