[PDF] Top 20 Polinomios Ortogonales. Propiedades y Aplicaciones
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Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
... the CRISPR/Cas9 system in two plasmids To establish a genome editing system in ...C. glutamicum based on CRISPR/Cas9, we designed and constructed a two-plasmid ... See full document
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A RecET-assisted CRISPR–Cas9 genome editing in Corynebacterium glutamicum
... functional Cas9-sgRNA complex is lethal to some bacteria lacking NHEJ pathway like eukaryotes [14, 39, ...of CRISPR–Cas9 system with either dsDNA with different HA lengths or ssDNA as ... See full document
10
Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum
... C. glutamicum more efficiently and unleash its potential in industrial biotechnology, facile yet robust genome editing tools are urgently ...The CRISPR/Cas9 system that utilized NGG as ... See full document
103
Development of a CRISPR-Cas9 System for Efficient Genome Editing of Candida lusitaniae
... the Cas9 and sgRNA scaffolds (see primers in Data Set ...by using 20-bp protospacers immediately followed by a PAM sequence of the structure NGG (or preceded by CCN if located on the reverse ...genome ... See full document
116
RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain
... the CRISPR interference for C. glutamicum To construct the two-plasmid system (pCoryne-dCas9 and pCoryne-sgRNA) of the CRISPRi (Table 1 and ...vector system [11] was used for expression of the ... See full document
14
Upgrading of efficient and scalable CRISPR–Cas-mediated technology for genetic engineering in thermophilic fungus Myceliophthora thermophila
... Cas12a-based CRISPR system for efficient multiplex genome editing, using a single-array approach in ...These CRISPR–Cas12a cassettes worked well for simultaneous multiple ... See full document
164
Targeted Editing of the StPDS Gene using the CRISPR/Cas9 system in Tetraploid Potato
... potato using traditional cross-breeding methods (Muthoni et ...are efficient, problems may emerge in the long run since the endogenous genes are still expressed, resulting in the potential instability of ... See full document
14
Optimizing CRISPR Cas9 Genome Editing System: A Review
... recombinant Cas9 protein was complexed with SgRNA or dual RNA consisting of crRNA and tracrRNA has been directly delivered into cells via electroporation and showed efficient genome editing in human ... See full document
5
Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum
... the editing efficiencies of our system for gene deletion in ...C. glutamicum, the upp gene was chosen as the target gene, since its deletion leads to a 5-fluoro- uracil ... See full document
263
CRISPR Cas9 Mediated Genome Editing in Leishmania donovani
... prokaryotic CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9, an RNA-guided endonu- clease, has been shown to mediate efficient genome editing in a wide variety of ...the ... See full document
11
Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Brassica napus L.) using CRISPR-Cas9 system
... towards CRISPR- Cas9-mediated cleavage in Brassica species, it is far from gene modification to comprehensive application in crop ...with CRISPR-Cas9- mediated mutations in ...that ... See full document
6
Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
... of CRISPR–Cas9 medi- ated genome editing system in markerless gene dele- tion, two genes OpLEU2 encoding the 3-isopropylmalate dehydrogenase and OpURA3 encoding the orotidine- 5 ′ ... See full document
122
An improved Tet-on system in microRNA overexpression and CRISPR/Cas9-mediated gene editing
... and efficient approach for gen- etic perturbation [37, ...and Cas9 expression cassettes, a cell line stably express- ing inducible Cas9 soon became ...perform CRISPR/Cas9 ex- periments ... See full document
7
In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing
... The CRISPR/Cas9 system facilitates site-specific cleavage of the chromosome from various ...the CRISPR/Cas9 system has been developed as a universal genome-editing tool ... See full document
72
Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 System
... Cas9-mediated nucleotide replacement. Targeted nucleotide substitution is widely used to characterize the functional roles of specific amino acids in a gene. To test whether a precise nucleotide FIG 4 ... See full document
124
Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila
... pyogenes system was simplified to two components: the Cas9 nuclease and a single chimeric guide RNA ...direct Cas9-mediated cleavage of both DNA strands within the target sequence (Jinek et ...the ... See full document
145
Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
... CRISPR/Cas9 system is an exciting breakthrough in DNA editing ...Repeat, CRISPR, is a bacte- rial acquired immune system to combat phage infections that has been intelligently ... See full document
13
A Multiplex CRISPR/Cas9 System for Use as an Anti-BmNPV Therapeutic
... one-vector CRISPR/Cas9 system for BmNPV genome excision BmNPV contains 141 genes most of which have not been studied [1, ...different gene knockouts on the replication of BmNPV, we constructed ... See full document
14
Editing the genome of Aphanomyces invadans using CRISPR/Cas9
... Although a large amount of data on the gross and microscopic clinical lesions of EUS and the involvement of different pathogens during the course of the disease already exist [9, 16–18], there still is a paucity of ... See full document
12
Keeping CRISPR/Cas on-Target
... Alternatively, method like Genome-wide Unbiased Indentification of DSBs evaluated by Sequencing (GUIDE- seq)is recently developed. GUIDE-seq and high-throughput genome-wide translocation sequencing (HTGTS) have proved to ... See full document
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