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Deleting multiple lytic genes enhances biomass yield and production of recombinant proteins by Bacillus subtilis
... delete multiple genes involved in cell lysis to improve biomass and the production of recombinant ...individually deleting skfA, sdpC, xpf [34], or lytC decreased cell lysis and ... See full document
18
Production of Chlamydia pneumoniae Proteins in Bacillus subtilis and Their Use in Characterizing Immune Responses in the Experimental Infection Model
... its proteins are difficult and ...chlamydial proteins in a heterologous host, Bacillus subtilis, a gram-positive nonpathogenic ...The genes of Chlamydia pneumoniae major outer membrane ... See full document
105
A new potential secretion pathway for recombinant proteins in Bacillus subtilis
... secrete recombinant proteins, many aspects of this pathway require further ...cytoplasmic proteins remains. Second, although the three reporter proteins can be transported to the extracellular ... See full document
111
Production and purification of alkaline protease from bacillus subtilis mr12 isolated from slaughter house soil
... From microscopic appearance and the biochemical tests, the isolate MR12 was identified as Bacillus sp. and further confirmation was done by the 16S rDNA sequence analysis. Upon the amplification of 16S rDNA ... See full document
126
Investigation on Microorganisms and their Degradation Efficiency in Paper and Pulp Mill Effluent
... move the dark color from the effluents. Of late industry follows either chemical oxidation/precipitation methods or biological methods for color removal. Chemical oxi- dation/precipitation methods are tedious, provide an ... See full document
16
Optimization and biodegradation of feather waste
... indole production, hydrogen sulphide production, nitrate reduction, methyl red, Voges- Proskauer, oxidative/fermentative test and utilization of carbon ... See full document
18
Cloning the hbs gene from Bacillus subtilis and expression of the HBsu protein in Escherichia coli
... The hbs gene has been cloned and expressed by many investigators and properties of recombinant protein investigated. Micka et al., (16) have cloned, sequenced and characterized the B.subtilis gene encoding the ... See full document
39
OPTIMIZATION STUDIES ON ALPHA AMYLASE PRODUCTION BY BACILLUS LICHENIFORMIS DS3 AND BACILLUS SUBTILIS DS7 USING SUBMERGED FERMENTATION
... enzyme production by submerged ...enzyme production was observed in tryptone containing the ...amylase production was maximum when yeast extract was used as a nitrogen source in Bacillus ... See full document
12
Identification and optimization of PrsA in Bacillus subtilis for improved yield of amylase
... B. subtilis increased the activity of AmyE, AmyS and AmyQ in the media in accordance with previous studies [19, 20], but had no significant effect on the other amylases tested in this ...PrsA enhances ... See full document
6
Characterization of proteins induced by mitomycin C treatment of Bacillus subtilis.
... The complexity of the mature PBSX particle, revealed both by electron microscopy and by its large number of structural proteins, suggests that additional proteins were synthesized during[r] ... See full document
12
The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease
... CP recombinant spores evoked plenty of specific sIgA in in- testinal mucosa and bile ...B. subtilis spores in orally administered mice, especially in the ... See full document
6
Comparative Analysis Of The Development Of Swarming Communities Of Bacillus Subtilis In Case Of Pta And ComXP Mutant Strains
... 929 ( comXP and pta). If we theoretically added the amount produced via comXP with that produced via pta, we notice that about 90% of surfactin production would be missing and only about 10% will be secreted by ... See full document
9
Control of Bacillus subtilis Replication Initiation during Physiological Transitions and Perturbations
... B. subtilis normally initiates when the cell contains complete, homologous chromosomes where the copy number represents a power of ...B. subtilis replication initiation control, which ties DnaA activity to ... See full document
82
High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system
... Plasmid pHY300PLK [26] was used to determine transfor- mation efficiency and plasmid pHY-WZX [27] was used as an expression vector in B. licheniformis. E. coli XL1 was used as a host cell for functional identification of ... See full document
180
Characterization and overexpression of a novel keratinase from Bacillus polyfermenticus B4 in recombinant Bacillus subtilis
... insoluble proteins with a robust structure, are a major component of epidermal tissue and appendages such as hair, feathers, nails, and ...lyse proteins with robust cross‑linked structures, such as keratin, ... See full document
17
77. Comparative study of alpha amylase producing mutated and unmutated Ba-cillus strains by taking cost effective measures
... Selection of an appropriate strain is impera- tive for enhanced production of alpha amyl- ase. In current research work soil samples were obtained from diverse starchy fields of Lahore. Screening of potent strains ... See full document
22
Antimicrobial Activity of Extracellular Proteins Secreted by Bacillus Subtilis ATCC21332 after being Induced with Cymbopogon Flexuosus Essential Oil and Cultured in Different Growth Conditions
... However, the extracellular proteins synthesized by B. subtilis ATCC21332 prior to treatment with 0.01 MIC of C. flexuosus essential oil and cultured in different pH media or supplied with variable nutrients ... See full document
499
Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors
... In an attempt to obtain a B. subtilis strain completely unre- sponsive to A. thaliana exudates, we evaluated the attraction of the mcpB mcpC double mutant. As shown in Fig. 3C, the mcpB mcpC mutant displayed about ... See full document
67
A COMPARATIVE STUDY ON THE PHB PRODUCTION BY BACILLUS SPECIES UNDER SOLID STATE FERMENTATION BY AGRICULTURAL WASTES
... PHB yield, make consortium of Bacillus species and to reduce the cost of production media along with suitable PHB induction media ...the production of PHB serving triple purpose of reducing ... See full document
16
EFFECT OF ETHYL PENTANOATE ON ANTI- QUORUM ACTIVITY OF EXTRACELLULAR PROTEIN SECRETED BY BACILLUS SUBTILIS ATCC11774
... subsequent production stage, in which the stationary phase where the primary nutrients source is depleted, the microbial secondary metabolites are ...the production of extracellular proteins and ... See full document
298
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