[PDF] Top 20 The system of patents in Colombia
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Ushering in CRISPR/Cas Mediated Genome Engineering for Crops
... the genome followed by repair by either non-homologous end joining (NHEJ) or homology-directed repair ...(CRISPR)/ CRISPR-associated protein (Cas) has been widely utilized for many plant ... See full document
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Generation of Acsl4 Gene Knockout Mouse Model by CRISPR/Cas9-Mediated Genome Engineering
... Acsl4 mediated by the CRISPR/ Cas9 system have not been reported in mice ...The CRISPR/Cas9 system has emerged as a new genome editing technology in ...the CRISPR/Cas9 ... See full document
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Upgrading of efficient and scalable CRISPR–Cas-mediated technology for genetic engineering in thermophilic fungus Myceliophthora thermophila
... the CRISPR components of the Cas12a and crRNA expression cassettes were able to function when introduced transiently and without direct ...the CRISPR– Cas12a cassettes were not integrated into the ... See full document
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Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering
... the CRISPR/Cas9 ...of CRISPR/Cas9 in medaka, however, has yet to be ...for genome digestion, an sgRNA targeted for donor plasmid digestion, and Cas9 ... See full document
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Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas
... whole genome sequencing and Sanger sequencing of potential off-target sites, it was also demonstrated that the sys- tem is highly specific in plants as no significant off-target activity was ... See full document
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Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas
... on CRISPR-directed nuclease activity offer the potential for maximally convenient engineering of target specificity to effect changes to the ...the CRISPR RNA (crRNA) and the trans-activating crRNA ... See full document
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CRISPR–Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1
... efficient genome editing tools leading to competent metabolic engineering strate- gies need to be ...developed. Genome editing by Clus- tered Regularly Interspaced Short Palindromic Repeats and the ... See full document
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CRISPR Cas9 MEDIATED GENOME EDITING IN ESCHERICHIA COLI
... the CRISPR-Cas9 genetic engineering technology. CRISPR-associated protein-9 (Cas9) is part of this immune system that seeks out, cuts, and degrades viral ...the CRISPR-Cas9 system, a tool has ... See full document
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CRISPR Cas9 Mediated Genome Editing in Leishmania donovani
... prokaryotic CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9, an RNA-guided endonu- clease, has been shown to mediate efficient genome editing in a wide variety of ...the ... See full document
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Advances in Engineering the Fly Genome with the CRISPR-Cas System
... fly genome is significantly changing the course of research in ...Cas9-mediated genome engineering and active-genetic strategies are likely to have a transformative impact on genetics as we ... See full document
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Applications of CRISPR-Cas9 mediated genome engineering
... in genome engineering based on the CRISPR-Cas9 system enables fast functional characterization of putative disease genes in various mouse models via somatic genome editing ...A CRISPR ... See full document
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CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae
... Methods that enable fast, sequential or combinato- rial integrations are valuable for metabolic engineering. Several powerful approaches, either plasmid- or PCR- based, have been demonstrated for genome ... See full document
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Phylogenetic Distribution of CRISPR Cas Systems in Antibiotic Resistant Pseudomonas aeruginosa
... interesting CRISPR-Cas-genomic island interactions observed in our data set (see Table S4 in the supplemental material), we looked closely at a widely distributed spacer targeting the pilV2 gene of the ... See full document
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Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system
... The DSB introduced by Cas9 nuclease guided by an sgRNA can be repaired via either the cell’ s NHEJ or HDR mecha- nisms. NHEJ can be harnessed to generate single and mul- tiple gene knock-outs as well as large chromosomal ... See full document
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CRISPR Cas Mediated Phage Resistance Enhances Horizontal Gene Transfer by Transduction
... transduce CRISPR-Cas immunity ...infection, CRISPR adaptation events could eventually lead to the extinction of transducing phages (44) and eliminate the potential to generate transducing particles ... See full document
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CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
... co- CRISPR approach is quicker initially because the repair template is a PCR product and no cloning is required, but isolating knock-ins takes more work because PCR screening of 50–100 animals is ... See full document
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Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing
... the CRISPR array. Whether other type VI CRISPR-Cas enzymes have the same acti- vation process of collateral cleavage and the same role of pre-crRNA processing in cleavage activity as Cas13a, is ... See full document
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CRISPR-Cas-Mediated Gene Silencing Reveals RacR To Be a Negative Regulator of YdaS and YdaT Toxins in Escherichia coli K-12
... Differential contribution of YdaS and YdaT to cell toxicity and morphological defects. In order to assess which one of the two (YdaS or YdaT) acts as a toxin or whether one of these two proteins serves as a cotoxin to ... See full document
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dCas9: A Versatile Tool for Epigenome Editing
... its size. The coding sequence of the widely used SpyCas9 is around 4.2 kb, which is compatible with the ~4.5 kb maximum accommodated in common viral vectors used for delivery in mammalian cells. However, such a long ... See full document
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CRISPR-Cas Targeting and Escape in Lactobacillus gasseri.
... that CRISPR was involved in heritable cell immunity through inferences based on the amino acid composition and domain functions of the Cas proteins (Makarova, Grishin, Shabalina, Wolf, & Koonin, ...that ... See full document
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