CONTRASTACIÓN DE LA HIPÓTESIS

*Assistant Professor, Department of Microbiology,

S.T.E.T Women’s College, Sundarakkottai, Mannargudi,

Tamil Nadu, INDIA.

Email id: [email protected] **Student

Department of Microbiology,

S.T.E.T Women’s College, Sundarakkottai, Mannargudi, Tamil Nadu, INDIA. Email id: [email protected]

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The application of tissue culture techniques to the regulation and commercial propagation of

hybrid roses is more recent developed. Rose is one of the most popular flowering ornamentals in

the world. Presently, it is a favorite ornamental for landscapes, as well as the most important

commercial cut flower. The present study was planned to in vitro propagation of Rosa damascena Mill. The present study was planned to establish the efficient protocol on in vitro micro propagation of Rosa damascena Mill, and manipulating growth regulating and culture conditions. The study was demonstrated the feasibility of propagating rose in vitro. On the other hand considerable reduction in the rate of contamination (10%) with 85% survival of explants was obtained by treating the explants with sodium hypochlorite (5%) for 10 minutes after rapid rinsing with ethanol (70%) for 30 s and mercuric chloride (0.1%) for 6 minutes. The active plants were selected and subjected to shoot initiation in MS medium supplemented with growth regulators BAP, IAA and 2, 4-D. The multiple shoot induction of Rosa damascena Mill was obtained from culturing a single nodal explants on MS medium supplemented with 4.0 mg/l BA and 0.1 mg/l NAA, giving 8.27 shoots per explants in 4 weeks which could then be repeatedly sub cultured the individual newly formed shoot on the same medium. At the end of 8 weeks, a multiplication rate of 59.8cm shoot from each initial explant was obtained. Rooting was induced by sub culturing the individual new shoot on MS medium supplemented with 0.5 mg/l NAA in 4

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weeks, giving 2.3 roots per shoot. After 12 weeks of growth, 90% of the plantlets obtained were established under outdoor conditions. Thus, the procedure could be used for the commercial propagation of Rosa damascena Mill. The results of our study showed that auxin IBA, IAA and NAA were the most important hormones for the form time roots and shoots.

KEYWORDS:In Vitro, Proliferation, Initiation, Regeneration, Micro Propagation, BAP, NAA. ______________________________________________________________________________ REFERENCES

1. Altman, A., Micropropagation of plants: principles and practices. In: Spier R. (Ed.)

Encyclopedia of Cell Technology. John Wiled and Sons Inc. New York, 2000; P: 916-

929.

2. Dhawan, V., and Bhojwani, S.S., Micropropagation in crop plants. Glimpses in Plant Res.,

1986; 7: 1-75.

3. Elliott, R.F., Axenic culture of meristem tips of Rosa multiflora planta, 1970; 95: 183-185. 4. Skirvin R.M., and Chu M.C., Young H.J., Rose. In: Ammirato P.V., Evans D.R., Sharp

W.R., Bajaj Y.P.S. (eds), Handbook of Plant Cell Culture, vol. 5; New York, 1990; 716-743. 5. Khan, I.A. and Shaw J.J., Biotechnology in Agriculture. Punjab. Agric. Res. Coordination

Board Faisalabad, Pakistan, 1988; P: 2.

6. Debergh, P.C., and Read P.E., Micropropagation. In: Micropropagation: Technology and

Application. (Ed.): P.C. Debergh and R.H. Zimmerman. Kluwer Acad. Publishers, London,

1991; P: 1-13.

7. Horn W.A.H., Micropropagation of rose. In: Bajaj Y.P.S (ed)., Agriculture and Forestry,

Berlin, Springer-Verlag, 1992; 4: 320-324.

8. Pati P.K., Rath S.P., Sharma M., Sood A., Ahuja P.S., In vitro propagation of rose: a review.

Biotechnology Advances, 2006; 24: 94-114.

9. Yan M., Byrne D.H., Jing C., Propagation of rose species in vitro. In vitro Cellular and Developmental Biology Plant, 1996; 32: 103-108.

10.Rout G.R., Samantaray S., Mottley J., Das P., Biotechnology of the rose: a review of recent progress. Scientia Horticultural, 1999; 81: 201-228.

11.Hasegawa P.M., In vitro propagation of rose. Horticultural Science, 1979;14: 610-612. 12.Barve D.M., Iyer R.S., Kendurkar S., Mascarenhas A.F., An efficient method for Rapid

Propagation of some budded rose varieties. Indian Journal ofHorticulture, 1984; 41: 1-7. 13.Chu C.Y., Knight S.L., Smith M.A.L., Effect of liquid culture on the growth and

development of miniature rose (Rosa damascena Mill L.). Plant Cell, Tissue and Organ

culture, 1993; 32: 329-334.

14.Voyiatzi C.I., An assessment of the in vitro germination capacity of pollen grains of five Tea

hybrid rose cultivars. Euphytica, 1995; 83: 199-204.

15.Murashige T., Skoog A., A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 1962; 15: 473-497.

16.Maryam Jafarkhani Kermani, Gorban Ali Nematzadeh Mohammad Reza Bihamta in vitro

propagationof biotechnology, 2007; 5:2.

17.Van der Salm, T.P.M., Van der Toorn, C.J.G., Hanisch ten Cate, C.H., Dubois, L.A.M., Devries, D.P., Dons, H.J.M., Importance of the iron chelate formula for micropropagation of

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18.Ebrahim M.K.H., Ibrahim A.I., Influence of medium solidification and pH value on in vitro

propagation of maranta leuconeura CV Kerchoviana. Sci Hortic, 2000; 86:211-221.

19.Ghashghaie, J., Brenckmann F., Saagier B., Effects of agar concentration on water status and growth of rose plants cultured in vitro. Physiol Plant, 1991; 82: 73-78.

20.Rout G.R., Debata B.K., Das P., In vitro clonal multiplication of roses. Proceedings of

National Acadamy of Sciences India, 1990; 60: 311-318.

21.Pati, P.K., Sharma, M., Sood, A., Ahuja, P.S., Direct shoot regeneration from leaf explants of

Rosa damascena Mill. In vitro Cell Dev Bio Plant 2004; 40(2): 192-195.

22.Dubois L.A.M., De Vries D.P., Preliminary report on the direct regeneration of adventitious buds on leaf explants of in vitro grown glass house rose cultivars. Gartenbauwissenschaft, 1995; 60(6): 249-253.

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