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Transition3.y5.1.03.y7.1.03.y8.1.0LOD0.550.973.65IS Area1.0E+052.0E+042.5E+04

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(1)

Response curves for sp|O43490|PROM1_HUMAN Peptide: AFTDLNSINSVLGGGILDR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

0.150.20.250.30.35

3.y5.1.0 3.y7.1.0 3.y8.1.0 Spike level : 1

Response curves (zoom) sp|O43490|PROM1_HUMAN Peptide: AFTDLNSINSVLGGGILDR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

012345 3.y5.1.03.y7.1.0 3.y8.1.0 Spike level : 1

Data points for sp|O43490|PROM1_HUMAN Peptide: AFTDLNSINSVLGGGILDR

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.150.20.250.30.350.4

3.y5.1.0 3.y7.1.0 3.y8.1.0 Spike level : 1

Transition 3.y5.1.0 3.y7.1.0 3.y8.1.0

LOD 0.55 0.97 3.65

IS Area

1.0E+05

2.0E+04

2.5E+04

(2)

Response curves for sp|O43490|PROM1_HUMAN Peptide: VLPIEQSLSTLYQSVK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

02468101214

2.y7.1.0 3.y6.1.0 3.y7.1.0 Spike level : 1

Response curves (zoom) sp|O43490|PROM1_HUMAN Peptide: VLPIEQSLSTLYQSVK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

01020304050

2.y7.1.0 3.y6.1.0 3.y7.1.0 Spike level : 1

Data points for sp|O43490|PROM1_HUMAN Peptide: VLPIEQSLSTLYQSVK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.20.512510 2.y7.1.0

3.y6.1.0 3.y7.1.0 Spike level : 1

Transition 2.y7.1.0 3.y6.1.0 3.y7.1.0

LOD 4.19 4.20 266.02

IS Area

3.6E+03

5.6E+03

4.5E+03

(3)

Response curves for sp|O43490|PROM1_HUMAN Peptide: YILAQYNTTK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

0500100015002000

2.y4.1.0 2.y7.1.0 2.y9.1.0 Spike level : 1

Response curves (zoom) sp|O43490|PROM1_HUMAN Peptide: YILAQYNTTK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

05101520

2.y4.1.0 2.y7.1.0 2.y9.1.0 Spike level : 1

Data points for sp|O43490|PROM1_HUMAN Peptide: YILAQYNTTK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.11101001000 2.y4.1.02.y7.1.0

2.y9.1.0 Spike level : 1

Transition 2.y4.1.0 2.y7.1.0 2.y9.1.0

LOD 10.02 22.59 1.90

IS Area

7.1E+03

5.6E+04

2.6E+04

(4)

Response curves for sp|P04216|THY1_HUMAN Peptide: HENTSSSPIQYEFSLTR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

051015202530 3.y4.1.0 3.y5.1.0 3.y6.1.0 Spike level : 1

Response curves (zoom) sp|P04216|THY1_HUMAN Peptide: HENTSSSPIQYEFSLTR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

0123456 3.y4.1.0

3.y5.1.0 3.y6.1.0 Spike level : 1

Data points for sp|P04216|THY1_HUMAN Peptide: HENTSSSPIQYEFSLTR

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.10.20.51251020 3.y4.1.03.y5.1.0

3.y6.1.0 Spike level : 1

Transition 3.y4.1.0 3.y5.1.0 3.y6.1.0

LOD 37.68 2.42 17.08

IS Area

4.4E+03

5.4E+04

3.3E+03

(5)

Response curves for sp|P04216|THY1_HUMAN Peptide: VLYLSAFTSK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

0123456

2.y6.1.0 2.y7.1.0 2.y8.1.0 Spike level : 1

Response curves (zoom) sp|P04216|THY1_HUMAN Peptide: VLYLSAFTSK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.10.20.3

2.y6.1.0 2.y7.1.0 2.y8.1.0 Spike level : 1

Data points for sp|P04216|THY1_HUMAN Peptide: VLYLSAFTSK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.00050.0050.050.515

2.y6.1.0 2.y7.1.0 2.y8.1.0 Spike level : 1

Transition 2.y6.1.0 2.y7.1.0 2.y8.1.0

LOD 0.12 0.09 0.01

IS Area

1.8E+05

1.6E+05

7.4E+05

(6)

Response curves for sp|P04216|THY1_HUMAN Peptide: VTSLTAC[+57]LVDQSLR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

00.020.040.06

2.y8.1.0 2.y9.1.0 2.y10.1.0 Spike level : 1

Response curves (zoom) sp|P04216|THY1_HUMAN Peptide: VTSLTAC[+57]LVDQSLR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.050.10.150.20.25 2.y8.1.0

2.y9.1.0 2.y10.1.0 Spike level : 1

Data points for sp|P04216|THY1_HUMAN Peptide: VTSLTAC[+57]LVDQSLR

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.0010.0020.0050.010.020.05

2.y8.1.0 2.y9.1.0 2.y10.1.0 Spike level : 1

Transition 2.y10.1.0 2.y8.1.0 2.y9.1.0

LOD 0.06 0.06 0.15

IS Area

1.2E+05

1.1E+05

9.4E+04

(7)

Response curves for sp|P16070|CD44_HUMAN Peptide: ALSIGFETC[+57]R

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

00.10.20.30.4

2.y5.1.0 2.y6.1.0 2.y8.1.0 Spike level : 1

Response curves (zoom) sp|P16070|CD44_HUMAN Peptide: ALSIGFETC[+57]R

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.020.040.060.080.1

2.y5.1.0 2.y6.1.0 2.y8.1.0 Spike level : 1

Data points for sp|P16070|CD44_HUMAN Peptide: ALSIGFETC[+57]R

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.00020.0010.0050.020.050.20.5

2.y5.1.0 2.y6.1.0 2.y8.1.0 Spike level : 1

Transition 2.y5.1.0 2.y6.1.0 2.y8.1.0

LOD 0.07 0.01 0.01

IS Area

3.9E+05

2.0E+06

2.0E+06

(8)

Response curves for sp|P16070|CD44_HUMAN Peptide: FAGVFHVEK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

01020304050 2.y5.1.0

2.y7.1.0 2.y8.1.0 Spike level : 1

Response curves (zoom) sp|P16070|CD44_HUMAN Peptide: FAGVFHVEK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.20.40.6

2.y5.1.0 2.y7.1.0 2.y8.1.0 Spike level : 1

Data points for sp|P16070|CD44_HUMAN Peptide: FAGVFHVEK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.010.050.5151050

2.y5.1.0 2.y7.1.0 2.y8.1.0 Spike level : 1

Transition 2.y5.1.0 2.y7.1.0 2.y8.1.0

LOD 0.90 0.35 0.95

IS Area

6.4E+04

9.7E+04

2.5E+04

(9)

Response curves for sp|P16070|CD44_HUMAN Peptide: TGSFGVTAVTVGDSNSNVNR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

0123

3.y5.1.0 3.y6.1.0 3.y7.1.0 Spike level : 1

Response curves (zoom) sp|P16070|CD44_HUMAN Peptide: TGSFGVTAVTVGDSNSNVNR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

01234

3.y5.1.0 3.y6.1.0 3.y7.1.0 Spike level : 1

Data points for sp|P16070|CD44_HUMAN Peptide: TGSFGVTAVTVGDSNSNVNR

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.050.10.20.512

3.y5.1.0 3.y6.1.0 3.y7.1.0 Spike level : 1

Transition 3.y5.1.0 3.y6.1.0 3.y7.1.0

LOD 1.77 1.76 3.38

IS Area

2.3E+04

1.1E+04

5.1E+04

(10)

Response curves for sp|P35226|BMI1_HUMAN Peptide: DGLTNAGELESDSGSDK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

020406080100

2.b6.1.0 2.y5.1.0 2.y7.1.0 Spike level : 1

Response curves (zoom) sp|P35226|BMI1_HUMAN Peptide: DGLTNAGELESDSGSDK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.511.522.53

2.b6.1.0 2.y5.1.0 2.y7.1.0 Spike level : 1

Data points for sp|P35226|BMI1_HUMAN Peptide: DGLTNAGELESDSGSDK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.050.5151050 2.b6.1.02.y5.1.0

2.y7.1.0 Spike level : 1

Transition 2.b6.1.0 2.y5.1.0 2.y7.1.0

LOD 3.23 1.44 4.13

IS Area

3.4E+03

7.8E+03

3.5E+03

(11)

Response curves for sp|P35226|BMI1_HUMAN Peptide: LVPGLFK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

0100200300

2.y4.1.0 2.y5.1.0 2.y6.1.0 Spike level : 1

Response curves (zoom) sp|P35226|BMI1_HUMAN Peptide: LVPGLFK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.511.52

2.y4.1.0 2.y5.1.0 2.y6.1.0 Spike level : 1

Data points for sp|P35226|BMI1_HUMAN Peptide: LVPGLFK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.10.5151050500

2.y4.1.0 2.y5.1.0 2.y6.1.0 Spike level : 1

Transition 2.y4.1.0 2.y5.1.0 2.y6.1.0

LOD 2.19 0.20 4.61

IS Area

2.1E+04

3.1E+05

1.3E+04

(12)

Response curves for sp|P48431|SOX2_HUMAN Peptide: APC[+57]QAGDLR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

020406080100

2.y5.1.0 2.y6.1.0 2.y7.1.0 Spike level : 1

Response curves (zoom) sp|P48431|SOX2_HUMAN Peptide: APC[+57]QAGDLR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.511.522.53

2.y5.1.0 2.y6.1.0 2.y7.1.0 Spike level : 1

Data points for sp|P48431|SOX2_HUMAN Peptide: APC[+57]QAGDLR

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.20.5125102050100

2.y5.1.0 2.y6.1.0 2.y7.1.0 Spike level : 1

Transition 2.y5.1.0 2.y6.1.0 2.y7.1.0

LOD 4.84 11.66 5.54

IS Area

2.1E+03

8.0E+02

9.0E+02

(13)

Response curves for sp|P48431|SOX2_HUMAN Peptide: LLSETEK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

010203040506070

2.y3.1.0 2.y5.1.0 2.y6.1.0 Spike level : 1

Response curves (zoom) sp|P48431|SOX2_HUMAN Peptide: LLSETEK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.511.522.53

2.y3.1.0 2.y5.1.0 2.y6.1.0 Spike level : 1

Data points for sp|P48431|SOX2_HUMAN Peptide: LLSETEK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.050.20.5125102050 2.y3.1.0

2.y5.1.0 2.y6.1.0 Spike level : 1

Transition 2.y3.1.0 2.y5.1.0 2.y6.1.0

LOD 3.15 0.62 0.60

IS Area

2.4E+03

1.2E+04

1.7E+04

(14)

Response curves for sp|P48431|SOX2_HUMAN Peptide: SEASSSPPVVTSSSHSR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

0102030405060

3.y7.1.0 3.y11.2.0 3.y15.2.0 Spike level : 1

Response curves (zoom) sp|P48431|SOX2_HUMAN Peptide: SEASSSPPVVTSSSHSR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.511.522.53

3.y7.1.0 3.y11.2.0 3.y15.2.0 Spike level : 1

Data points for sp|P48431|SOX2_HUMAN Peptide: SEASSSPPVVTSSSHSR

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.020.050.20.5125102050

3.y7.1.0 3.y11.2.0 3.y15.2.0 Spike level : 1

Transition 3.y11.2.0 3.y15.2.0 3.y7.1.0

LOD 0.10 0.09 10.31

IS Area

3.6E+04

5.2E+04

1.1E+04

(15)

Response curves for sp|P48681|NEST_HUMAN Peptide: AHADDELAALR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

020406080 2.y9.1.0

3.b5.1.0 3.y4.1.0 Spike level : 1

Response curves (zoom) sp|P48681|NEST_HUMAN Peptide: AHADDELAALR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.20.40.60.81 2.y9.1.03.b5.1.0

3.y4.1.0 Spike level : 1

Data points for sp|P48681|NEST_HUMAN Peptide: AHADDELAALR

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.050.5151050 2.y9.1.0 3.b5.1.0 3.y4.1.0 Spike level : 1

Transition 2.y9.1.0 3.b5.1.0 3.y4.1.0

LOD 0.09 0.19 0.04

IS Area

3.2E+05

1.8E+05

5.6E+05

(16)

Response curves for sp|P48681|NEST_HUMAN Peptide: DVEVVRPLEK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

050100150200250300350

2.y7.1.0 2.y8.1.0 3.y8.2.0 Spike level : 1

Response curves (zoom) sp|P48681|NEST_HUMAN Peptide: DVEVVRPLEK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.511.52 2.y7.1.0

2.y8.1.0 3.y8.2.0 Spike level : 1

Data points for sp|P48681|NEST_HUMAN Peptide: DVEVVRPLEK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.10.5151050

2.y7.1.0 2.y8.1.0 3.y8.2.0 Spike level : 1

Transition 2.y7.1.0 2.y8.1.0 3.y8.2.0

LOD 0.61 0.65 0.13

IS Area

2.4E+04

2.8E+04

2.0E+05

(17)

Response curves for sp|P48681|NEST_HUMAN Peptide: VGLNAQAAC[+57]APR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

0123456 2.y6.1.0 2.y7.1.0 2.y8.1.0 Spike level : 1

Response curves (zoom) sp|P48681|NEST_HUMAN Peptide: VGLNAQAAC[+57]APR

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.020.040.060.08

2.y6.1.0 2.y7.1.0 2.y8.1.0 Spike level : 1

Data points for sp|P48681|NEST_HUMAN Peptide: VGLNAQAAC[+57]APR

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.0050.020.050.10.20.5125

2.y6.1.0 2.y7.1.0 2.y8.1.0 Spike level : 1

Transition 2.y6.1.0 2.y7.1.0 2.y8.1.0

LOD 0.02 0.03 0.06

IS Area

5.5E+05

5.3E+05

3.7E+05

(18)

Response curves for sp|Q01860|PO5F1_HUMAN Peptide: FEALQLSFK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

0510152025 2.y6.1.02.y7.1.0

2.y8.1.0 Spike level : 1

Response curves (zoom) sp|Q01860|PO5F1_HUMAN Peptide: FEALQLSFK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

00.20.40.60.8 2.y6.1.02.y7.1.0

2.y8.1.0 Spike level : 1

Data points for sp|Q01860|PO5F1_HUMAN Peptide: FEALQLSFK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.10.20.51251020 2.y6.1.0

2.y7.1.0 2.y8.1.0 Spike level : 1

Transition 2.y6.1.0 2.y7.1.0 2.y8.1.0

LOD 1.36 0.38 2.08

IS Area

8.0E+03

3.1E+04

6.3E+03

(19)

Response curves for sp|Q01860|PO5F1_HUMAN Peptide: LEQNPEESQDIK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

020004000600080001000012000 2.b4.1.0

2.y5.1.0 2.y8.1.0 Spike level : 1

Response curves (zoom) sp|Q01860|PO5F1_HUMAN Peptide: LEQNPEESQDIK

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

01020304050

2.b4.1.0 2.y5.1.0 2.y8.1.0 Spike level : 1

Data points for sp|Q01860|PO5F1_HUMAN Peptide: LEQNPEESQDIK

Peak Area Ratio

0.1 0.5 1 5 10 50 100

510505005000

2.b4.1.0 2.y5.1.0 2.y8.1.0 Spike level : 1

Transition 2.b4.1.0 2.y5.1.0 2.y8.1.0

LOD 52.35 80.52 63.49

IS Area

2.1E+03

2.4E+03

2.1E+03

(20)

Response curves for sp|Q01860|PO5F1_HUMAN Peptide: VFSQTTIC[+57]R

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 50 100 150 200

020040060080010001200

2.y3.1.0 2.y5.1.0 2.y7.1.0 Spike level : 1

Response curves (zoom) sp|Q01860|PO5F1_HUMAN Peptide: VFSQTTIC[+57]R

Theoretical Concentration (fmol/µg)

Peak Area Ratio

0 0.2 0.4 0.6 0.8 1

0246810

2.y3.1.0 2.y5.1.0 2.y7.1.0 Spike level : 1

Data points for sp|Q01860|PO5F1_HUMAN Peptide: VFSQTTIC[+57]R

Peak Area Ratio

0.1 0.5 1 5 10 50 100

0.5151050500

2.y3.1.0 2.y5.1.0 2.y7.1.0 Spike level : 1

Transition 2.y3.1.0 2.y5.1.0 2.y7.1.0

LOD 2.78 3.58 20.59

IS Area

7.8E+03

1.1E+04

9.1E+03

Referencias

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