[PDF] Top 20 La investigación biográfica, estrategia de educación para la salud
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A molecular scheme for improved characterization of human embryonic stem cell lines
... all human genes, it is possible to measure the expression of undifferentiated markers and any con- ceivable differentiation marker in a single ...these molecular methods is that the cells are assayed as an ... See full document
15
The Molecular Karyotype of 25 Clinical-Grade Human Embryonic Stem Cell Lines
... ES cell lines were cultured in feeder-free conditions prior to the isolation of genomic DNA ...hESC lines (Table 1) was assayed for single nucleotide polymorphisms (SNPs) using the Illumina ... See full document
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Identification and single-cell functional characterization of an endodermally biased pluripotent substate in human embryonic stem cells
... entiated stem cells (Figure ...of stem cell ...for stem cell ...unsorted stem cell line (Figure 4A), and expressed similar levels of core stem cell ... See full document
103
Human Embryonic Stem Cell Lines Model Experimental Human Cytomegalovirus Latency
... latency reactivation in vivo (15). In this article, we show that when either clinical (FIX) or laboratory-adapted (AD169) strains of HCMV enter ESCs, tegument-delivered pp71 localizes to the cy- toplasm, Daxx remains ... See full document
149
Improved hematopoietic differentiation of human pluripotent stem cells via estrogen receptor signaling pathway
... ng/ml stem cell factor (SCF), 10 ng/ml interleukin-3 (IL-3), 10 ng/ml IL-6, 50 ng/ ml granulocyte colony stimulating factor (G-CSF), and 300 ng/ml ... See full document
7
Human development, heredity and evolution
... to human embryos combined with single-cell RNA sequencing and in vitro culture techniques is opening the door to human developmental ...germ cell and brain development. A precise understanding ... See full document
15
Cytotrophoblast stem cell lines derived from human embryonic stem cells and their capacity to mimic invasive implantation events
... effective embryonic–maternal interaction is crucial for successful human ...cytotrophoblast stem (CTBS) cell lines from human embryonic stem cells ...CTBS ... See full document
113
Genomic instability of human embryonic stem cell lines using different passaging culture methods
... Teratoma-forming potential and tumor content by histological analysis were explored in the H1 and H9 cell lines. This teratoma assay enabled clear-cut evaluation of the impact of both dissociation protocols ... See full document
12
Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes
... Real-Time Cell Analysis (RTCA) Cardio system, which utilizes impedance tech- nology to quantify CM-beating properties, has been previously reported as an emerging method to quantify cardiac contractility ... See full document
12
Embryonic germ cells from mice and rats exhibit properties consistent with a generic pluripotent ground state
... EG cell formation appears to recapitulate this conversion in vitro but without genetic ...somatic cell reprogramming (Takahashi and Yamanaka, ...and molecular transitions that mediate derestriction ... See full document
25
Defining stemness of human embryonic stem cells: a systems biology approach
... of embryonic and foetal stem cells although safety and efficiency of these transplantations are not known [100, ...with stem cell treatments: a patient with lupus nephritis (a disease of the ... See full document
5
Genetic modification increases the survival and the neuroregenerative properties of transplanted neural stem cells
... Neural stem cells (NSCs) or precursor cells are usually transplanted at the site of injury to restore function in damaged brain ...of cell death are characteristic for predifferentiated transplanted cells: ... See full document
8
Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES 7
... dently-derived human embryonic stem cell (hESC) lines remains challenging due to considerable diversity in culture methods practiced between ...mouse embryonic fibroblast (MEF) ... See full document
56
Biomimetics in dentistry, review and recent update
... regenerative Endodontic methods for regeneration of lost tooth or its structures can only be achieved with such procedures. Thus, these methods have the potential for regenerating both pulp & dentin offering an ... See full document
9
Human Embryonic Stem Cell (hESC) and Human Embryo Research
... The use of excess IVF embryos creates other ethical issues. Couples un- dergoing IVF may be both emotionally and fi nancially vulnerable. Care must be taken to ensure that the informed consent process for research ... See full document
12
Human Embryonic Stem Cell (hESC) and Human Embryo Research
... a cell line. Because of the unique self- renewing capacity of stem cells, the lines can often be maintained in- de fi ...a human embryo, federal research grants involving hESCs entail the ex- ... See full document
9
Gene therapy for colorectal cancer using adenovirus-mediated full-length antibody, cetuximab
... empty and Hu5-empty were used as controls. HEK293 cell culture supernatants were harvested after 24 h. Supernatants and Erbitux were separated on 10% SDS- PAGE under reducing or non-reducing conditions. The DiFi ... See full document
458
High-efficiency derivation of human embryonic stem cell lines using a culture system with minimized trophoblast cell proliferation
... capacity, human embryonic stem cells (hESCs) have great potential for studying developmental biology, disease modeling, and developing cell replacement ...inner cell mass (ICM) cells ... See full document
347
Strain difference in establishment of mouse embryonic stem (ES) cell lines
... Int J D BioI 38 385 390 (1994) 385 Strain difference in establishment of mouse embryonic stem (ES) cell lines EIHACHIRO KAWASE", HIROFUMI SUEMORI' ', NOBUKO TAKAHASHI, KEIKO OKAZAKI', KOICHIRO HASHIMO[.] ... See full document
40
Vitamin D3 stimulates embryonic stem cells but inhibits migration and growth of ovarian cancer and teratocarcinoma cell lines
... Human carcinoma cells were treated in RPMI/0.5 % BSA medium containing either 1,25-dihydroxyvitamin D3 (10 −10 M, 10 −9 M, or 10 −8 M) or vehicle only. Cells subjected to culture medium with 10 % DMSO were used as ... See full document
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