[PDF] Top 20 Leopoldo Zea, El Pensamiento Latinoamericano
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Protein secretion in Lactococcus lactis: an efficient way to increase the overall heterologous protein production
... low secretion yields or absence of secretion was observed in ...L. lactis. In all cases, fusion to a SP stabilizes heterolo- gous protein production even though they are not effi- ... See full document
10
A propeptide toolbox for secretion optimization of Flavobacterium meningosepticum endopeptidase in Lactococcus lactis
... for heterologous protein ...an efficient recombinant protein expression toolbox is required, especially for recombinant protein ...enhancing protein secretion consist ... See full document
302
Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis
... rHtrA protein was found to be stable in the culture supernatant of the otherwise WT host strain (Fig- ures 2, 3 and 4a, lane 1 and Figure 4b, lane ...many heterologous and/or recombinant proteins are ... See full document
106
Crystal Structure of the Receptor-Binding Protein Head Domain from Lactococcus lactis Phage bIL170
... Lactococcus lactis, a gram-positive bacterium widely used by the dairy industry, is subject to lytic phage infections. In the first step of infection, phages recognize the host saccharidic receptor using ... See full document
5
Molecular Verification of Two Potent Bacteria Isolated from Darfiyeh Cheese: Lactococcus lactis Subsp Lactis and Lactobacillus plantarum
... (LAB): Lactococcus lactis subsp. lactis and Lactobacillus plantarum will be ...as Lactococcus lactis subsp. lactis and Lactobacillus plantarum by implementing specie-specific ... See full document
217
Nisin inducible production of listeriolysin O in Lactococcus lactis NZ9000
... total protein quantitation To obtain rapid and reproducible purification of LLO, we used the Ni-NTA technology to capture and purify the six- His-tagged protein by affinity chromatography under native ... See full document
109
Cell surface engineering of Bacillus subtilis improves production yields of heterologously expressed α-amylases
... the secretion process was also investigated. The secretion efficiency of α-amylases with different pIs was tested in the vari- ous mutant strains and the enzymatic activity assay in combination with Western ... See full document
185
Construct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine
... Accurate molecular mass of full-length R0.6C was meas- ured by LC–ESI–MS. 30 pmol of protein were loaded on a C4 pre-column (Acquity UPLC Protein BEH C4 Van- guard, 1.7, 2.1 × 5 mm, Waters, USA) and eluted ... See full document
13
Comprehensive analysis of the HEPN superfamily: identification of novel roles in intra-genomic conflicts, defense, pathogenesis and RNA processing
... these nucleases are deployed as part of a multi-pronged defense strategy against the enterobacteriophage T4 (and most likely against other, related phages). Although PrrC and RloC are both anticodon nucleases (ACNases), ... See full document
7
Secretion of biologically active pancreatitis-associated protein I (PAP) by genetically modified dairy Lactococcus lactis NZ9000 in the prevention of intestinal mucositis
... the secretion of antimicrobial ...L. lactis NZ9000 strain expressing or not PAP was able to reduce eosinophil ...L. lactis NCC2287, was used for the treatment of Eosinophilic esophagitis in ... See full document
107
Lactococcus lactis provides an efficient platform for production of disulfide-rich recombinant proteins from Plasmodium falciparum
... L. lactis for expression and purification of a panel of 31 recombinant proteins from 25 distinct anti- gens of ...surface protein complexes play impor- tant roles in erythrocyte invasion and several of them ... See full document
7
In vivo imaging of Lactococcus lactis, Lactobacillus plantarum and Escherichia coli expressing infrared fluorescent protein in mice
... L. lactis and ...L. lactis resulted in considerable standard ...bacterial secretion was in agreement with other approaches that were used (ex vivo imaging of intestines, fluorescence ... See full document
20
Molecular Insights on the Recognition of a Lactococcus lactis Cell Wall Pellicle by the Phage 1358 Receptor Binding Protein
... bacterium Lactococcus lactis is used for the production of cheeses and other fermented dairy ...L. lactis cells by virulent lactococcal tailed phages is one of the major risks of fermentation ... See full document
66
Autolytic and Proteolytic Properties of Strains of Lactococcus lactis Isolated from Different Vegetables, Raw Milk Cheeses and Commercial Starter Cultures
... L. lactis had different behavior depending on the lysis solution ...a way that some con- ditions enhanced the autolysis of certain strains while disfavored the autolysis of other strains (Figure ...L. ... See full document
82
Another Brick in the Wall: a Rhamnan Polysaccharide Trapped inside Peptidoglycan of Lactococcus lactis
... Rhamnan biosynthesis is encoded in the same chromosomal gene cluster as the PSP. Comparison of the gene cluster encoding CWPS synthesis in L. lactis strains MG1363 and 3107 revealed a conserved 5=-located region ... See full document
103
Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor
... rNuc produced and secreted by strain 918 at the highest level (after induction of cells grown in GM17v to an OD 600 of 2 by 500 μM EDTA) was purified from the cul- ture medium using a simple, previously-described single ... See full document
148
Heterologous production of human papillomavirus type 16 L1 protein by a lactic acid bacterium
... L. lactis-derived VLPs and L1 protein were recovered as follows: 10 ml of ...induced-L. lactis cultures were pelleted and resuspended in 3 ml of either PBS pH ...L1 protein were applied on ... See full document
12
Recombinant Lactococcus lactis NZ9000 secretes a bioactive kisspeptin that inhibits proliferation and migration of human colon carcinoma HT-29 cells
... functional protein delivery vectors has been widely investigated [6]. Lactococcus lactis, a LAB model species, has been demonstrated to be a promising candi- date for the delivery of functional ... See full document
67
SdrF, a Staphylococcus aureus surface protein, binds type I collagen
... L. lactis binding to type I Cn via its B ...ofL. lactis cells harboring recombinant plasmids from panel ...L. lactis NZ9000 harboring cloning vector ...ofL. lactis strains frompanel B to type ... See full document
122
Expanding the recombinant protein quality in Lactococcus lactis
... destroy protein function [5]. Since protein production processes have to meet not only good cost-effectiveness ratios but also high product quality [6-8], the use of cell factories other than ... See full document
118
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