[PDF] Top 20 Necesidad de las humanidades, su lugar en la universidad
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Detection of E. coli O157:H7 in Meat Using Polymerase Chain Reaction Method and Culture Method
... amplified using one pair of specific primers (Table ...DNA polymerase (Takara, Japan), and distilled deionized water up to a volume of 25 ...PCR reaction, thermal cycling was carried out in AB 2720 ... See full document
9
An Alternative Method to Concentrate E. coli O157:H7 from Meat Homogenates to Facilitate Detection by Cultural and Molecular Methods
... with E. coli O157:H7 (~10 4 CFU/ml), reported approximately 55% recovery of cells for subsequent PCR ...of E. coli O157:H7 with considerable variability in recovery ... See full document
19
Development and Performance Evaluation of an Antibody-Based Technology for Detection of E. coli O157 in Meat Samples and Its Potential Evolution Using Antibody Engineering
... cells using their fimbriae or pili, as the rest of the pathogenic ...E. coli do ...serotype, E. coli O157:H7 was discovered in 1982 during an outbreak related to ... See full document
300
Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk
... enterohaemorrhagic E. coli secreted proteins A, B and D (EspA, EspB, and EspD) which are important in signal transduc- tion of mammalian host cells as well as attaching and effacing lesion formation ... See full document
147
Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis
... of E. coli O157: H7 outbreaks understanding ...identify E. coli, and shiga toxin producing E. coli using molecular methods as culture based ... See full document
18
Rectoanal Mucosal Swab Culture Is More Sensitive Than Fecal Culture and Distinguishes Escherichia coli O157:H7 Colonized Cattle and Those Transiently Shedding the Same Organism
... Escherichia coli O157:H7 was first identified as a hu- man pathogen (27, 33), investigations have demonstrated that human disease outbreaks are often linked to a bovine food source or bovine ... See full document
18
Shiga Toxin Producing Escherichia coli in Children: Diagnosis and Clinical Manifestations of O157:H7 and Non O157:H7 Infection
... for O157 STEC is frequently performed by stool culture with sorbitol-MacConkey agar ...Most O157 STEC strains do not rapidly ferment sorbitol, so sorbitol-neg- ative colonies on SMAC at 24 h are ... See full document
36
5′ Nuclease PCR Assay To DetectYersinia pestis
... the polymerase processes the newly synthesized leading strand, it encounters the bound probe and cleaves individual nucleotides from the 59 end (eventually, the probe is ...the polymerase, its fluores- ... See full document
21
Detection and Identification of Mycobacterium Species Isolates by DNA Microarray
... As an alternative to 16S rRNA analysis, Yamamoto and Harayama (44, 45, 46) designed a set of PCR primers that allowed both the amplification of the gyrB gene, which encodes the subunit B protein of DNA gyrase ... See full document
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Serotype O157:H7 Escherichia coli from bovine and meat sources
... coli serotype 0157:H7 possessed a large-molecular-size plasmid 53 to 64 MDa but only 5 of them possessed the ability to adhere to HEp-2 and Intestine 407 cells indicates that in vitro ex[r] ... See full document
8
Escherichia coli O157:H7 Biofilm Formation and Susceptibility to Disinfectants In Vitro and in Footbaths.
... of E. coli O157:H7 may be more resistant to disinfectants than others, leading to the possibility of sudden outbreaks (Wang et ...Additionally, E. coli O157:H7 ... See full document
89
Antimicrobial effects of curcumin against L. monocytogenes S. aureus S. Typhimurium and E. coli O157:H7 pathogens in minced meat
... minced meat and control were analysed 0–7 days ...and E. coli O157:H7 and ...aureus, E. coli O157:H7 and ...and E. coli O157:H7 ... See full document
179
Effect of Electron Beam Irradiation on Survival of Escherichia coli O157:H7 and Salmonella enterica serovar Thyphimurium in Minced Camel Meat during Refrigerated Storage
... irradiation method is safer and more affordable (Farkas and Mohácsi-Farkas, 2011; Kanatt et ...effective method to destroy spoilage and pathogenic microorgan- isms, without affecting nutritional and ... See full document
10
Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and hemolytic uremic syndrome
... Detection of Shiga toxin-producing Shigella dysenteriae type 1 and Escherichia coli by using polymerase chain reaction with incorporation of digoxigenin-11dUTP.. Nucleotide sequence anal[r] ... See full document
52
Carbapenem and Fluoroquinolone Resistance in Multidrug Resistant Pseudomonas aeruginosa Isolates from Al-Zahra Hospital, Isfahan, Iran
... extracted using a DNA extraction kit (Sinaclon) according to the manufacturer’s instructions and were used as template for PCR ...by using the primers described in table ... See full document
274
Novel planar interdigital sensors for the detection of bacterial endotoxins : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philososphy in Electronics Engineering at Massey University, Palmerston North, New Zealand
... The prepared NaCl solutions have different molarity between 0.01 M and 0.1 M. All sensors were coated with thin Incralac (< 5 μm) and cured overnight at room temperature. Incralac has been widely used as a coating ... See full document
84
Use of a reamplification protocol improves sensitivity of detection of Mycobacterium tuberculosis in clinical samples by amplification of DNA
... We have compared the sensitivity and specificity of quantitative mycobacterial culture against results obtained by using the polymerase chain reaction for the detection of DNA from organ[r] ... See full document
57
Polymerase Chain Reaction Detection and Restriction Enzyme Typing of Human Papillomavirus in Cervical Carcinoma
... Polymerase Chain Reaction Detection and Restriction Enzyme Typing of Human Papillomavirus in Cervical Carcinoma ORIGINAL ARTICLE Polymerase Chain Reaction Detection and Restriction Enzyme Typing of Hu[.] ... See full document
74
Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... direct detection limit in which resulting statistical differences were being identified, but based on the current statistical result analysis of Salmonella in fish can be faster if sample DNA is extracted by ... See full document
85
New Insights into the Formation of Viable but Nonculturable Escherichia coli O157:H7 Induced by High Pressure CO2
... buffer B for 20 min, and 60% to 100% buffer B for 2 min. The system was then maintained with 100% buffer B for 1 min before equilibration with 5% buffer B for 10 min prior to the next injection. Elution was monitored by ... See full document
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