[PDF] Top 20 Nivel de satisfacción de los usuarios del servicio de agua potable de la ciudad de Cajamarca, 2015.
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Antibodies for Venezuelan Equine Encephalitis Virus Protect Embryoid Bodies from Chikungunya Virus
... Chikungunya virus (CHIKV) is an alphavirus vectored by Aedes mosquitos. In humans, CHIKV can cause a febrile illness punctuated by severe polyarthralgia. Historically, CHIKV infection was thought to be ... See full document
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Vaccination of Macaques against Pathogenic Simian Immunodeficiency Virus with Venezuelan Equine Encephalitis Virus Replicon Particles
... derived from SFV, another alphavirus, have been tested in two macaque ...anti-gp160 antibodies detectable by ELISA but showed no neutralizing antibodies or T-cell prolif- erative ...protected ... See full document
5
Pseudoinfectious Venezuelan Equine Encephalitis Virus: a New Means of Alphavirus Attenuation
... attenuated virus mutants incapable of inducing disease or the use of inactivated ...attenuated virus vaccines offer many advantages over the use of inactivated and subunit ...neutralizing antibodies ... See full document
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Structure of Isolated Nucleocapsids from Venezuelan Equine Encephalitis Virus and Implications for Assembly and Disassembly of Enveloped Virus
... results from the noniso- tropic expansion of the isolated nucleocapsid relative to the mature-virus nucleocapsid and the redistribution of density that occurs throughout the inner surface of the ... See full document
11
Identification of Cross-Reactive Norovirus CD4+ T Cell Epitopes
... Stimulation with overlapping peptide libraries facilitates identification of T cell epitopes. Immunization of mice with VRPs containing norovirus ORF2 sequences results in an an- tinorovirus immune response characterized ... See full document
1
Expression and Self-Assembly of Norwalk Virus Capsid Protein from Venezuelan Equine Encephalitis Virus Replicons
... . Forty amplification cycles were performed, with one cycle consisting of 30 s at 94°C, annealing at 55°C for 1 min, and primer extension at 68°C for 2 min. While the NV1 sequence was identical to the published sequence ... See full document
9
Replicon Particles of Venezuelan Equine Encephalitis Virus as a Reductionist Murine Model for Encephalitis
... Flow cytometry analysis revealed extensive infiltration of blood-borne leukocytes and proliferation of brain-resident mi- croglia cells in the infected brain (Fig. 6 and 7), and accumu- lation of these cells around ... See full document
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Expression of the Two Major Envelope Proteins of Equine Arteritis Virus as a Heterodimer Is Necessary for Induction of Neutralizing Antibodies in Mice Immunized with Recombinant Venezuelan Equine Encephalitis Virus Replicon Particles
... protein labeling mix; NEN Life Science Products, Inc.) for 2 h. At 12 h postin- fection, cells were washed once in ice-cold PBS and solubilized in 500 l of solubilization buffer as described above. The cytoplasmic ... See full document
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Testosterone correlates with Venezuelan equine encephalitis virus infection in macaques
... to Venezuelan equine encephalitis virus (VEEV) in macaques which were used as part of a larger study conducted by the Department of Defense to better characterize pathological responses to ... See full document
8
Temperature-sensitive Steps in the Biosynthesis of Venezuelan Equine Encephalitis Virus
... It appeared that pre- I, i virus multiplication was reversibly inhibited incubation at 37 C by-passed the early tempera- at 44 C; ii no IRNA synthesis occurred; and ture-sensitive step a[r] ... See full document
Searching for the proverbial needle in a haystack: advances in mosquito-borne arbovirus surveillance
... Sugar-based surveillance has several advantages over traditional methods. When mosquito populations are el- evated, sorting becomes time consuming, and a high number of pools can overwhelm laboratory capacity. When ... See full document
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Doxorubicin and paclitaxel enhance the antitumor efficacy of vaccines directed against HER 2/neuin a murine mammary carcinoma model
... Fixation, permeabilization, and staining with FITC-labeled anti-IFN-γ, with peridinin chlorophyll protein-conjugated anti-CD3 and with phycoerythrin-conjugated anti-CD8 monoclonal antibodies (BD Pharmingen ... See full document
7
Role of Dendritic Cell Targeting in Venezuelan Equine Encephalitis Virus Pathogenesis
... At later time points, virus spreads to DC that are strongly positive for DEC 205 in the paracortex of the draining lymph node. DEC 205 is typically associated with interdigitating DC, a subset of DC which is ... See full document
7
Glycosaminoglycan Binding Properties of Natural Venezuelan Equine Encephalitis Virus Isolates
... derived from cDNA clones, in- dicate that GAG binding plays little or no role in the epizootic ...comes from the epizootic subtype IC strain 3908, which was isolated during a major VEE epi- demic/epizootic ... See full document
5
Locking and Blocking the Viral Landscape of an Alphavirus with Neutralizing Antibodies
... human Venezuelan equine encephalitis virus (VEEV), an alphavirus, in complex with two strongly neutralizing antibody Fab fragments (F5 and 3B4C-4) have been determined using a combination of ... See full document
The Crystal Structures of Chikungunya and Venezuelan Equine Encephalitis Virus nsP3 Macro Domains Define a Conserved Adenosine Binding Pocket
... ADP-ribose 1 ⴖ -phosphate phosphatase activity. ADP-ribose 1 ⬙ -phosphate phosphatase was the first activity detected for the yeast Poa1p macro domain based on a genome-wide search of this specific activity (28). In ... See full document
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A putative receptor for Venezuelan equine encephalitis virus from mosquito cells.
... input virus, showing that these cells bind virus much more efficiently than do C6/36 cells under the conditions ...VEE virus to C6/36 and BHK cells was dose dependent but not saturable ...of ... See full document
Cellular and Humoral Immune Responses to Alphavirus Replicon Vaccines Expressing Cytomegalovirus pp65, IE1, and gB Proteins
... VEE virus is especially attractive because they express heterologous proteins to high levels (30), target expression to dendritic cells (22), and are capable of inducing both humoral and cellular immune responses ... See full document
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Ultra sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito borne viruses and parasites
... pathogens from the epidemic areas to new ones poses a serious threat to the monitoring, prevention, and control of disease ...RNA, virus-like particles, and plasmids were established as positive or limit of ... See full document
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Protein Phosphatase 1α Interacts with Venezuelan Equine Encephalitis Virus Capsid Protein and Regulates Viral Replication through Modulation of Capsid Phosphorylation
... Loss of PP1 inhibits VEEV replication. PP1 siRNA knockdown was performed to determine if PP1 is important for VEEV replication. U87MG astrocytoma cells were transfected with an siRNA pool targeting 4 sites in PP1 ␣ , PP1 ... See full document
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