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An in-vitro transcription assay for development of Rotavirus VP7
... of VP7 is from 49 to 1029 nt and this extra nucleotide is out of the translation ...ter transcription efficiency was found when one extra nucleotide (A, T, C, and G) was placed before HDV ribozyme sequence ... See full document
64
Assembly of Highly Infectious Rotavirus Particles Recoated with Recombinant Outer Capsid Proteins
... the VP7 layer (58), immunoelectron microscopy localizing VP4 in the cytoplasm between viroplasms and the ER membrane (46), colocalization of VP4 with NSP4 by immunofluorescence mi- croscopy and biochemical assays ... See full document
10
Polyadenylation of Influenza Virus mRNA Transcribed In Vitro from Model Virion RNA Templates: Requirement for 5′ Conserved Sequences
... the development of two independent assays which demonstrate for the first time that exogenous model RNA templates based on influenza virus virion RNA (vRNA) are transcribed in vitro to produce ... See full document
Carbohydrates Facilitate Correct Disulfide Bond Formation and Folding of Rotavirus VP7
... of VP7 with manip- ulated ...of rotavirus-infected cells to the reducing agent DTT inhibits formation of correct intradisulfide bonds on VP7 and that prolonged exposure permanently misfolds ... See full document
Expression of the rotavirus SA11 protein VP7 in the simple eukaryote Dictyostelium discoideum.
... present VP7 in a conformation similar to that seen on the mature ...SA11 VP7 is recognized by only one neutralizing antibody, whereas coinfection of cells with herpes simplex virus type 1 expressing SA11 ... See full document
106
ATP Is Required for Correct Folding and Disulfide Bond Formation of Rotavirus VP7
... the rotavirus glycoproteins is the VP7 outer capsid protein, which is a luminal and ER-associated polypeptide with only N-linked high-mannose oligosaccharide residues ...The VP7 protein of group A ... See full document
7
VP7 and VP4 Genotyping of Human Group A Rotavirus in Buenos Aires, Argentina
... of rotavirus P strains can be accomplished by identifi- cation of genetically different VP4 genes by reverse transcrip- tion-PCR (RT-PCR), as previously reported (9, 17, 33, 52, ...prevalent VP7 and VP4 ... See full document
101
Cloning of Rotavirus Outer Capsid Protein (VP7) Gene into the pGEM Vector
... $EVWUDFW ,QKXPDQVWKHJURXS$URWDYLUXVHVDUHDVVRFLDWHGZLWKHQGHPLFGLDUUKHDLQFKLOGUHQ XQGHUWKHDJHRIOHDGLQJWRDSSUR[LPDWHO\GHDWKVHYHU\\HDU,QWURGXFWLRQRIURWDYLUXV YDFFLQHV LQWR FKLOGKRRG LPPXQL]D[r] ... See full document
84
NS35 and not vp7 is the soluble rotavirus protein which binds to target cells.
... Having established that the rotavirus protein in cell lysates which adheres to susceptible cell surfaces is NS35, the gene 8 product, and not vp7, the gene 9 product, we next sought to d[r] ... See full document
70
Molecular analysis of outer capsid glycoprotein (VP7) genes from two isolates of human group C rotavirus with different genome electropherotypes
... The VP7 genes amplified from clinical isolates by RT-PCR were extracted with phenol-chloroform and precipitated with ...the VP7 genes from OK118 and OK450 with digoxigenin-11-dUTP (Boehringer, Mannheim, ... See full document
5
Antigenic and Genomic Diversity of Human Rotavirus VP4 in Two Consecutive Epidemic Seasons in Mexico
... In this study, the interaction of an HRV strain with more than one VP4 MAb was scored as positive when the second or third MAb recognized the virus with at least one-half the reactivity obtained with the MAb with the ... See full document
17
Two forms of VP7 are involved in assembly of SA11 rotavirus in endoplasmic reticulum.
... The cells were lysed in immunoprecipitation lysis buffer, and one half of each sample was immunoprecipitated with 72A4, an anti-virus-associated VP7 monoclonal antibody lanes 1 through 6[r] ... See full document
88
Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis.
... Comparison of the amino acid sequences of these two VP7 proteins with those already determined for other rotavirus strains reveals extensive sequence conservation between serotypes with [r] ... See full document
30
Development of a novel equine combination anthelmintic and a study of anthelmintic susceptibility using a larval development assay : a thesis presented in partial fulfilment of the requirements for the degree of Master of Philosophy in Veterinary Science at Massey University
... Gill et al., (1 995) reported in detail on their use of an agar matrix containing the anthelmintic. This is probably the same as that reported earlier by Lacey et al., (1 990). They cultured nematode eggs in a nutritive ... See full document
45
Characterization of a Novel P[25],G11 Human Group A Rotavirus
... of rotavirus isolates from pigs or other animals have been conducted thus ...G11 VP7 specificity in a human patient raises the question whether this strain was of animal ... See full document
74
Production of reassortant viruses containing human rotavirus VP4 and SA11 VP7 for measuring neutralizing antibody following natural infection.
... gene profile was achieved, i.e., no or very little detectable Wa or ST3 gene 9 or RV5 gene 8 and no detectable SA11 gene 4. This goal was achieved by neutral- ization both prior to and during infection or during ... See full document
8
In vitro transcription catalyzed by heat-treated human rotavirus.
... To test whether a similar situation exists in human rotaviruses, we examined the effect of ribonucleoside triphosphate analogs on viral transcription.. Replacement of ATP by its analog /[r] ... See full document
7
Distribution of serotypes of human rotavirus in different populations
... The recent development of monoclonal antibodies MAbs that specifically and selectively bind to the VP7 glycoprotein of each of the four most-common rotavirus serotypes serotypes 1 to 4 h[r] ... See full document
104
Genetic relatedness among human rotavirus genes coding for VP7, a major neutralization protein, and its application to serotype identification
... Relationships among human rotavirus genes coding for VP7 as determined by hybridization of genomic dsRNAs to reassortant rotavirus probes under stringent conditions' Strain VP7 hybrid ba[r] ... See full document
66
Comparisons of rotavirus VP7 typing monoclonal antibodies by competition binding assay
... MAb RV4:3, which has been used to recognize antigenic variants within VP7 type 1 strains, showed reciprocal competition with the four VP7 type 3 MAbs RV3:1, YO-lE2, 4F8, and 159 using a [r] ... See full document
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