[PDF] Top 20 Reutilización de la información generada en el sector público español
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Dynamic Localization of Trypanosoma brucei Mitochondrial DNA Polymerase ID
... essential mitochondrial DNA polymerase that localizes to the antipodal sites and to the kDNA ...or DNA repair. The multiplicity and spatial separation of the T. brucei mito- chondrial ... See full document
195
Mitochondrial DNA Ligases of Trypanosoma brucei
... The mitochondrial DNA of Trypanosoma brucei, termed kinetoplast DNA or kDNA, consists of thousands of minicircles and a small number of maxicircles catenated into a single network ... See full document
12
Contribution of Cytidine Deaminase to Thymidylate Biosynthesis in Trypanosoma brucei: Intracellular Localization and Properties of the Enzyme
... of Trypanosoma brucei CDA as an enzyme within the tetrameric class of the CDA family that efficiently deaminates cyt- idine, deoxycytidine, and the nucleoside analogue ...T. brucei proliferation when ... See full document
10
Mitochondrial and Nucleolar Localization of Cysteine Desulfurase Nfs and the Scaffold Protein Isu in Trypanosoma brucei
... of DNA polymerases (25) and other cytosolic and nuclear ...T. brucei, where they are essential and produce functional cofactors, even though the de- mand for Fe-S clusters within this organelle is ... See full document
31
Three Mitochondrial DNA Polymerases Are Essential for Kinetoplast DNA Replication and Survival of Bloodstream Form Trypanosoma brucei
... T. brucei, the technique is remarkably less successful in BF parasites (10 ⫺7 to 10 ⫺8 ) (6, 23, 29, ...three DNA polymerases, we found that parasites that survived RNAi (pro- liferated in clonogenic ... See full document
5
<p>Antileishmanial and antitrypanosomal activity of symmetrical dibenzyl-substituted α,β-unsaturated carbonyl-based compounds</p>
... of Trypanosoma brucei brucei wild type (WT) as well as ...b. brucei clonal line B48 and strong activity against Leishmania promastigotes, exceeding the ef fi cacy of the positive ... See full document
33
Invivo Anti Trypanosomal Activity of Aqueous Extractof Azadirachta indica Leaves on Trypanosoma brucei brucei Infected Mice
... of Trypanosoma brucei which cause different variants of ...T. brucei gambiense which causes slow onset chronic trypanosomiasis in ...T. brucei rhodesiense which cause fast onset acute ... See full document
17
Haematological indices in Trypanosoma brucei brucei (Federe isolate) infected Nigerian donkeys (Equus asinus) treated with homidium and isometamidium chloride of ciprofloxacin in broiler chickens after single intravenous and intraingluvial administration
... T. brucei brucei (Federe isolate) developed symptoms of trypanosomosis which included; anaemia, lymphocytosis and ...T. brucei brucei (Federe isolate) with haematological changes which ... See full document
12
Anti-trypanosomal evaluation of Ximenia americana root bark and chromatographic-mass spectrometric profile
... In an attempt to determine molecular weight to charge ratio (m/z) of various bands observed on TLC plate, the different peaks at a given retention time as shown in the TLC-ESI/MS chromatogram were considered (Fig. 3a). ... See full document
18
Trypanosoma brucei Tb927.2.6100 Is an Essential Protein Associated with Kinetoplast DNA
... T. brucei PF cells, several protein candidates were selected based on the following criteria: (i) identification of at least two tryptic peptides, (ii) absence of a known function (“hypothetical con- served” ... See full document
11
Nanomolar Inhibitors of Trypanosoma brucei RNA Triphosphatase
... The fact that the tertiary structures, active sites, and chemical mechanisms of the TTM-type RTPases are completely different from those of mammalian cysteine-phosphatase-type RTPases highlights TTM RTPases as promising ... See full document
10
Chalcone, Acyl Hydrazide, and Related Amides Kill Cultured Trypanosoma brucei brucei
... Li et al. (13) showed that chalcone (1,3- diphenyl-2-propen-one) derivatives inhibit the major cysteine proteinase of P. falciparum (falci- pain) and inhibit growth of both chloroquine- resistant and -sensitive P. ... See full document
13
ATP Synthase Is Responsible for Maintaining Mitochondrial Membrane Potential in Bloodstream Form Trypanosoma brucei
... other mitochondrial complex subunits in the procyclic stage have shown a similar decrease in the mitochondrial membrane potential ...T. brucei, as in other organisms, a proton-motive force has been ... See full document
10
Downregulation of Mitochondrial Porin Inhibits Cell Growth and Alters Respiratory Phenotype in Trypanosoma brucei
... (13, 43). The supernatant and pellet fractions were collected after centrifugation and analyzed by SDS-polyacrylamide gel electrophoresis (PAGE) and immuno- blotting. Protease digestion of isolated mitochondria was ... See full document
12
PCR Assay for Monitoring Trypanosoma cruzi Parasitemia in Childhood after Specific Chemotherapy
... Trypanosoma cruzi infection affects about 15 million people in the Americas. Thirty percent of these patients may develop myocardial disease early in life. Improved triatominal vector control is being achieved in ... See full document
137
A comparative evaluation of PCR based methods for species specific determination of African animal trypanosomes in Ugandan cattle
... often DNA is not spread homogenously over the matrix ...of DNA from the FTA card can improve the sensitivity of species-specific PCR reactions targeting trypanosomes ...of DNA from the FTA card to ... See full document
10
An Intragenic Guide RNA Location Suggests a Complex Mechanism for Mitochondrial Gene Expression in Trypanosoma brucei
... T. brucei indicate that not all maxicircle genes are transcribed as part of a polycistronic ...T. brucei maxicircle is much more compact and is thought to encode only two gRNAs (27) ...T. brucei ... See full document
5
Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei
... Compared to L. donovani, L. infantum and T. cruzi, all the strains of Trypanozoon were very closely related and clustered together in one sub-branch of the phylogenetic tree (Fig. 3A). The phylogenetic relationships ... See full document
15
Enigmatic Presence of Mitochondrial Complex I in Trypanosoma brucei Bloodstream Forms
... The ␣ subcomplexes from BFs and PFs comigrated on hrCNE, as did the  subcomplexes, suggesting that their subunit compo- sitions are quite similar in both stages. Indeed, mass spectrometric analysis of LIR-V5 pulldowns ... See full document
7
Characterization and Developmentally Regulated Localization of the Mitochondrial Carrier Protein Homologue MCP6 from Trypanosoma brucei
... The GenBank (gb), EMBL (emb) and Swissprotein (sp) accession numbers are as follows: HsapGDC, Homo sapiens sp_P16260; BtauGDC, Bos taurus sp_Q01888; ScerLEU5, Saccharomyces cerevisiae NP_011865; CeleGDC, Caeno- rhabditis ... See full document
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