[PDF] Top 20 Urban sprawl and housing interventions: the case of the city of Córdoba (Argentina)
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Generation of Cashmere Goats Carrying an EDAR Gene Mutant Using CRISPR-Cas9-Mediated Genome Editing
... common gene-editing techniques used involve the zinc finger nucleases, transcription activator-like effector nucleases, and the most recently developed clustered regularly interspaced short palindromic ... See full document
8
Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/Cas9-Mediated Gene Editing
... of mutant virus can be generated by CRISPR/Cas9 mutagenesis in as few as three pas- ...by Cas9 on the viral genome, indel mutations at a Cas9 cleavage site are generated by ... See full document
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Upgrading of efficient and scalable CRISPR–Cas-mediated technology for genetic engineering in thermophilic fungus Myceliophthora thermophila
... the CRISPR components of the Cas12a and crRNA expression cassettes were able to function when introduced transiently and without direct ...the CRISPR– Cas12a cassettes were not integrated into the ... See full document
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Generation of Acsl4 Gene Knockout Mouse Model by CRISPR/Cas9-Mediated Genome Engineering
... target gene in molecular biology. Genetic knockout of gene is the most reliable technique of study of loss-of-function of gene with high efficiency and specificity for in vivo analysis; however, the ... See full document
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A simple cloning free method to efficiently induce gene expression using CRISPR/Cas9
... of CRISPR/Cas technology presents promising opportunities to address these ...induce gene expression at an endogenous locus us- ing CRISPR/Cas9 ...nuclease-null Cas9 com- plex fused ... See full document
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Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9 Mediated Counterselection
... and CRISPR/Cas9-mediated counterselection in ...and Cas9. Targeting of Cas9 to a locus of interest is achieved by cloning a 20-bp DNA oligonucleotide (protospacer element) into the ... See full document
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Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
... Repeat, CRISPR, is a bacte- rial acquired immune system to combat phage infections that has been intelligently adapted for biotechnology purposes ...[11–13]. CRISPR/Cas9 from Streptococcus pyo- genes ... See full document
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Editing the genome of Aphanomyces invadans using CRISPR/Cas9
... Aphanomyces invadans growth and zoospore production Aphanomyces invadans strain 9701 was used in this study. It is routinely grown on glucose-peptone-yeast (GPY) agar and maintained on glucose-peptone (GP) agar at 25 °C ... See full document
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A Multiplex CRISPR/Cas9 System for Use as an Anti-BmNPV Therapeutic
... one-vector CRISPR/Cas9 system for BmNPV genome excision BmNPV contains 141 genes most of which have not been studied [1, ...different gene knockouts on the replication of BmNPV, we constructed ... See full document
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Rapid, Selection Free, High Efficiency Genome Editing in Protozoan Parasites Using CRISPR Cas9 Ribonucleoproteins
... endogenous gene tagging. The ability to efficiently knock out gene function by the insertion of stop codons and sequence tags delivered with single-stranded repair templates suggested that SaCas9/sgRNA ... See full document
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Advances in engineering CRISPR-Cas9 as a molecular Swiss Army knife
... base editing was found to have occurred at these sites and indel formation was significantly less than that induced by Cas9-mediated ...to Cas9 were extended to 32 and 9 aa, respectively, and ... See full document
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CRISPR/Cas9 mediated gene deletion of the ompA gene in symbiotic Cedecea neteri impairs biofilm formation and reduces gut colonization of Aedes aegypti mosquitoes
... Mono-association in Ae. aegypti mosquitoes were done using gnotobiotic infection procedure [7, 64], with slight modifications. Briefly, mosquito eggs were sterilized for 5 min in 70% etha- nol, 3 min in 3% ... See full document
260
CRISPR–Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics
... Genome editing allows for the precise manipulation of DNA sequences in a cell making this technology essential for understanding gene ...function. CRISPR/Cas9 is a targeted ... See full document
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Host Pathogen Wars: New Weapons from Biotechnology and Genomics
... These gene editing technologies have opened new doors to the study and mi- tigation of plant ...susceptibility gene Os 11 N 3 that interacts with Avr7 or PhXo3 effectors, disabling the compatible in- ... See full document
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Genome Editing of Babesia bovis Using the CRISPR/Cas9 System
... bovis genome, including several ex- panded gene families which are unique for Babesia ...the CRISPR/Cas9 system as a robust genetic engineering tool for ... See full document
306
CRISPR-Cas12a (Cpf1): a versatile tool in the plant genome editing tool box for agricultural advancement
... relaxase gene with Cas9 improved HDR mechanism in rice which facilitated closeness of repair template to DSBs in ...homology-directed gene targeting-HGT (Shaked et ...prime editing allows us ... See full document
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Epithelial mesenchymal transition leads to NK cell–mediated metastasis specific immunosurveillance in lung cancer
... role in maintaining metastatic latency (47). Supporting this, clinical studies in multiple solid tumors demonstrated an inverse correlation between the number of circulating or tumor-infiltrating NK cells and the ... See full document
8
Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 System
... the Cas9/sgRNA complex, we designed two sgRNAs to target the 3= end of the Pysera1 exon 2, generating plasmids pYC-sera1- sgRNA1 and ...specifically mediated by the Cas9/sgRNA ...2 ... See full document
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Keeping CRISPR/Cas on-Target
... assaying Cas9 binding include in cellulo ChIP (chromatin immunoprecipitation) and invitro SELEX (systematic evolution of ligands by exponential amplification) (shown in Figure ...next generation sequencing) ... See full document
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Vigour of CRISPR/Cas9 Gene Editing in Alzheimer’s Disease
... including gene editing ...the CRISPR-associated nuclease 9 (CRISPR/ Cas9) are the three gene editing tools procurable for ...the CRISPR/Cas9 because it is ... See full document
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