[PDF] Top 20 Cultural Management Challenges, in Latin America
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CoverView: a sequence quality evaluation tool for next generation sequencing data.
... present CoverView, a quality evaluation tool for NGS data that was designed to be user-friendly, fast, flexible and easy to integrate into NGS analysis ...pipelines. CoverView ... See full document
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Determining the quality and complexity of next generation sequencing data without a reference genome
... of sequencing reads, identi- fication of point mutations, whole genome assembly, searches for homologous regions and phylogenetic ana- lysis ...in sequencing data using a k- mer ...and quality ... See full document
83
Development and evaluation of a non ribosomal random PCR and next generation sequencing based assay for detection and sequencing of hand, foot and mouth disease pathogens
... genome sequence is sufficient for establishment of the diagnostic ...of next-generation sequencing based assay as a diagnostic tool was an objective in many recent reports ...addition, ... See full document
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Metagenomic next-generation sequencing as a diagnostic tool for toxoplasmic encephalitis
... Alignment tool [6], high-quality sequenc- ing data were aligned with Microbial Genome Databases, which is composed of 2700 whole genome sequences of viral taxa, 1494 bacterial genomes or scaffolds, ... See full document
83
Targeted next generation sequencing as a tool for precision medicine
... by sequencing cluster (n = ...base quality score (Phred scale) per read for all sequencing runs (n = ...within sequencing reads (n = ...targeted sequence for CES1 and CBR1 showing the ... See full document
5
Rapid detection of human herpes virus by next generation sequencing in a patient with encephalitis
... and sequencing DNA was extracted directly from the clinical samples using the TIANamp Micro DNA Kit (DP316, Tiangen Biotech, Beijing, ...DNA. Quality control was carried out using a bioanalyzer (Agilent ... See full document
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VariantDB: a flexible annotation and filtering portal for next generation sequencing data
... new data are available, all variants are re-annotated using the new ...maintain data traceability, all discarded annotations are archived and all changes to variant annotation are ...LiftOver tool, ... See full document
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Whole-genome sequencing and characterization of an antibiotic resistant Neisseria meningitidis B isolate from a military unit in Vietnam
... multi-locus sequence typing (MLST) scheme was first suggested by Maiden and colleagues in 1998 [11] utilizing seven house-keeping genes abcZ, adk, aroE, fumC, gdh, pdhC and pgm is now widely used to clas- sify ... See full document
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Genome-wide mutation analysis of Helicobacter pylori after inoculation to Mongolian gerbils
... genome sequence of TN2 wild type strain (TN2wt) and next generation sequencing data of retrieved strains from the animals after different lengths of ... See full document
64
Analysis of error profiles in deep next generation sequencing data
... This study was focused on error profiling but not vari- ant detection, although error suppression will ultimately improve variant detection. Variant detection can be for- mulated into three related but distinct study ... See full document
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Overcoming bias and systematic errors in next generation sequencing data
... the data correlates with a technical variable, such as processing date, location or ...DNA sequencing carried out as part of the 1000 Genomes Project ...this sequencing data, Leek et al. ... See full document
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pTuneos: prioritizing tumor neoantigens from next-generation sequencing data
... training data used in Neopep- see lack specificity as the peptides come from generic antigens rather than true noeantigens with experimental validation, and (3) the training data used in Neopepsee are ... See full document
5
Estimating Individual Admixture Proportions from Next Generation Sequencing Data
... However, having a low depth can bias the results of the admixture analysis as we have shown through simulations. In our simulations we have shown that basing the analysis on genotype likelihoods always outperforms the ... See full document
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Adaptive bandwidth kernel density estimation for next generation sequencing data
... Although our analysis shows that the choice of the ker- nel function – Gaussian, square, or triangle – has little influence on tuning or test-set probabilities, the choice does impact the computational resources needed ... See full document
212
Next-generation sequencing approach to hyperCKemia
... the EFNS criteria) were consecutively referred to the neu- rology, pediatric, or neuropediatric units of 7 Italian tertiary neuromuscular centers for clinical and diagnostic purposes. All 66 met the inclusion criteria ... See full document
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Next Generation Sequencing in the UMC Groningen
... enough data available for a reliable analysis. Historical data from before the introduction of the static schedule does not seem useful, since the situations are barely ... See full document
379
Next generation sequencing in thyroid cancer
... poor quality and quantity, can lead to false negative ...DNA quality of cytology specimen is better than that of FFPE tissue, cytology specimens would con- tain some amount of normal tissue ...Also, ... See full document
9
Quantifying Population Genetic Differentiation from Next-Generation Sequencing Data
... uneven sequencing cover- age among individuals (Figure ...of data is discarded from the analyses, potentially leading to loss of important features of the ... See full document
76
Unraveling genomic variation from next generation sequencing data
... DNA sequencing is carried ...a sequencing library consisting of such fragments of known insert size is created, while during a third step, these fragments are sequenced and finally mapped back to an already ... See full document
8
The Quality Sequencing Minimum (QSM): providing comprehensive, consistent, transparent next generation sequencing data quality assurance.
... the quality control of depth of coverage, base quality and mapping quality during the design, optimisation and utilisa- tion of the ...the Quality Sequencing Minimum ...minimum ... See full document
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